PMID- 29938906 OWN - NLM STAT- MEDLINE DCOM- 20190816 LR - 20190816 IS - 1615-9861 (Electronic) IS - 1615-9853 (Linking) VI - 18 IP - 15 DP - 2018 Aug TI - Colonic Mucosal Proteome Signature Reveals Reduced Energy Metabolism and Protein Synthesis but Activated Autophagy during Anorexia-Induced Malnutrition in Mice. PG - e1700395 LID - 10.1002/pmic.201700395 [doi] AB - Anorexia nervosa is an eating disorder often associated with intestinal disorders. To explore the underlying mechanisms of these disorders, the colonic proteome was evaluated during activity-based anorexia. Female C57Bl/6 mice were randomized into three groups: Control, Limited Food Access (LFA) and Activity-Based Anorexia (ABA). LFA and ABA mice had a progressive limited access to food but only ABA mice had access to an activity wheel. On colonic mucosal protein extracts, a 2D PAGE-based comparative proteomic analysis was then performed and differentially expressed proteins were identified by LC-ESI-MS/MS. Twenty-seven nonredundant proteins that were differentially expressed between Control, LFA, and ABA groups were identified. ABA mice exhibited alteration of several mitochondrial proteins involved in energy metabolism such as dihydrolipoyl dehydrogenase and 3-mercaptopyruvate sulfurtransferase. In addition, a downregulation of mammalian target of rapamycin (mTOR) pathway was observed leading, on the one hand, to the inhibition of protein synthesis, evaluated by puromycin incorporation and mediated by the increased phosphorylation of eukaryotic elongation factor 2, and on the other hand, to the activation of autophagy, assessed by the increase of the marker of autophagy, form LC3-phosphatidylethanolamine conjugate/Cytosolic form of Microtubule-associated protein 1A/1B light chain 3 (LC3II/LC3I) ratio. Colonic mucosal proteome is altered during ABA suggesting a downregulation of energy metabolism. A decrease of protein synthesis and an activation of autophagy were also observed mediated by mTOR pathway. CI - (c) 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. FAU - Nobis, Severine AU - Nobis S AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - Achamrah, Najate AU - Achamrah N AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Nutrition Department, Rouen University Hospital, 76000, Rouen, France. FAU - Goichon, Alexis AU - Goichon A AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - L'Huillier, Clement AU - L'Huillier C AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - Morin, Aline AU - Morin A AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - Guerin, Charlene AU - Guerin C AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - Chan, Philippe AU - Chan P AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Platform in proteomics PISSARO, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - do Rego, Jean Luc AU - do Rego JL AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Animal Behaviour Platform SCAC, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - do Rego, Jean Claude AU - do Rego JC AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Animal Behaviour Platform SCAC, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - Vaudry, David AU - Vaudry D AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Platform in proteomics PISSARO, UNIROUEN, Normandie University, 76000, Rouen, France. AD - INSERM Unit 1239, UNIROUEN, Normandie University, 76000, Rouen, France. FAU - Dechelotte, Pierre AU - Dechelotte P AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Nutrition Department, Rouen University Hospital, 76000, Rouen, France. FAU - Belmonte, Liliana AU - Belmonte L AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Nutrition Department, Rouen University Hospital, 76000, Rouen, France. FAU - Coeffier, Moise AU - Coeffier M AUID- ORCID: 0000-0002-7469-2682 AD - INSERM Unit 1073, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Institute for Research and Innovation in Biomedicine, UNIROUEN, Normandie University, 76000, Rouen, France. AD - Nutrition Department, Rouen University Hospital, 76000, Rouen, France. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180715 PL - Germany TA - Proteomics JT - Proteomics JID - 101092707 RN - 0 (Proteome) MH - Animals MH - Anorexia/*complications MH - *Autophagy MH - Colon/*metabolism MH - *Energy Metabolism MH - Female MH - Intestinal Mucosa/*metabolism MH - Malnutrition/etiology/metabolism/*pathology MH - Mice MH - Mice, Inbred C57BL MH - *Protein Biosynthesis MH - Proteome/*metabolism MH - Tandem Mass Spectrometry OTO - NOTNLM OT - activity-based anorexia OT - autophagy OT - colon OT - mammalian target of rapamycin OT - mitochondria OT - protein metabolism EDAT- 2018/06/26 06:00 MHDA- 2019/08/17 06:00 CRDT- 2018/06/26 06:00 PHST- 2017/10/25 00:00 [received] PHST- 2018/06/12 00:00 [revised] PHST- 2018/06/26 06:00 [pubmed] PHST- 2019/08/17 06:00 [medline] PHST- 2018/06/26 06:00 [entrez] AID - 10.1002/pmic.201700395 [doi] PST - ppublish SO - Proteomics. 2018 Aug;18(15):e1700395. doi: 10.1002/pmic.201700395. Epub 2018 Jul 15.