PMID- 29939171
OWN - NLM
STAT- MEDLINE
DCOM- 20180822
LR  - 20190125
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 136
DP  - 2018 Jun 8
TI  - The TreadWheel: Interval Training Protocol for Gently Induced Exercise in 
      Drosophila melanogaster.
LID - 10.3791/57788 [doi]
LID - 57788
AB  - The incidence of complex metabolic diseases has increased as a result of a 
      widespread transition towards lifestyles of increased caloric intake and lowered 
      activity levels. These multifactorial diseases arise from a combination of 
      genetic, environmental, and behavioral factors. One such complex disease is 
      Metabolic Syndrome (MetS), which is a cluster of metabolic disorders, including 
      hypertension, hyperglycemia, and abdominal obesity. Exercise and dietary 
      intervention are the primary treatments recommended by doctors to mitigate 
      obesity and its subsequent metabolic diseases. Exercise intervention, in 
      particular aerobic interval training, stimulates favorable changes in the common 
      risk factors for Type 2 Diabetes Mellitus (T2DM), Cardiovascular Disease (CVD), 
      and other conditions. With the influx of evidence describing the therapeutic 
      effect exercise has on metabolic health, establishing a system that models 
      exercise in a controlled setting provides a valuable tool for assessing the 
      effects of exercise in an experimental context. Drosophila melanogaster is a 
      great tool for investigating the physiological and molecular changes that result 
      from exercise intervention. The flies have short lifespans and similar mechanisms 
      of metabolizing nutrients when compared to humans. To induce exercise in 
      Drosophila, we developed a machine called the TreadWheel, which utilizes the 
      fly's innate, negative geotaxis tendency to gently induce climbing. This enables 
      researchers to perform experiments on large cohorts of genetically diverse flies 
      to better understand the genotype-by-environment interactions underlying the 
      effects of exercise on metabolic health.
FAU - Lowman, Kelsey E
AU  - Lowman KE
AD  - Department of Biological Sciences, University of Alabama.
FAU - Wyatt, Brelahn J
AU  - Wyatt BJ
AD  - Department of Biological Sciences, University of Alabama.
FAU - Cunneely, Owen P
AU  - Cunneely OP
AD  - Department of Biological Sciences, University of Alabama.
FAU - Reed, Laura K
AU  - Reed LK
AD  - Department of Biological Sciences, University of Alabama; lreed1@ua.edu.
LA  - eng
GR  - R01 GM098856/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20180608
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Drosophila/genetics
MH  - Drosophila melanogaster/*pathogenicity
MH  - Humans
MH  - Metabolic Syndrome/*therapy
MH  - Obesity/*therapy
MH  - Physical Conditioning, Animal/*methods
PMC - PMC6101642
EDAT- 2018/06/26 06:00
MHDA- 2018/08/23 06:00
PMCR- 2018/06/08
CRDT- 2018/06/26 06:00
PHST- 2018/06/26 06:00 [entrez]
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2018/08/23 06:00 [medline]
PHST- 2018/06/08 00:00 [pmc-release]
AID - 57788 [pii]
AID - 10.3791/57788 [doi]
PST - epublish
SO  - J Vis Exp. 2018 Jun 8;(136):57788. doi: 10.3791/57788.