PMID- 29939985
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20191210
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 6
DP  - 2018
TI  - Inactivation of Pichia rhodanensis in relation to membrane and intracellular 
      compounds due to microchip pulsed electric field (MPEF) treatment.
PG  - e0198467
LID - 10.1371/journal.pone.0198467 [doi]
LID - e0198467
AB  - The effect of microchip pulsed electric field (MPEF) treatment on lethal and 
      sublethal injury of Pichia rhodanensis (P. rhodanensis) were employed under 
      100-500 V for 20-100 pulses and the underlying mechanism of MPEF treatment was 
      investigated as well. A 6.48 log10 reduction of P. rhodanensis was achieved at 
      500V for 80 pulse. The fluorescent staining with Propidium Iodide (PI) verified 
      that the rate of sublethal injury cells maximum up to 27.2% under 200 V. MPEF can 
      cause the damage of cell morphology and ultrastructure, meanwhile causing a 
      decrease in cellular enzymes, antioxidant enzyme activity and cell membrane 
      fluidity. The leakage of intracellular compounds (protein, nucleic acid, K+, 
      Mg2+) and Ca2+-ATPase gradually increased as the growth of voltage, especially 
      the proportion of protein in the supernatants increased from 2.0% to 26.4%. Flow 
      cytometry analysis showed that MPEF has significant effect on membrane potential, 
      but no obvious influence on non-specific esterase. MPEF can cause the changing of 
      the secondary structure of protein, at the same time, double helix structure of 
      DNA became loose and unwinding. These results provide a theoretical guidance for 
      the widespread using of MPEF technology in the application of a non-thermal 
      processing technique for food.
FAU - Zhu, Ning
AU  - Zhu N
AD  - Department of Food Science and Engineering, College of Biological Sciences and 
      Technology, Beijing Forestry University, Beijing, China.
FAU - Yu, Ning
AU  - Yu N
AD  - Department of Food Science and Engineering, College of Biological Sciences and 
      Technology, Beijing Forestry University, Beijing, China.
FAU - Zhu, Yue
AU  - Zhu Y
AD  - Department of Food Science and Engineering, College of Biological Sciences and 
      Technology, Beijing Forestry University, Beijing, China.
FAU - Wei, Yulong
AU  - Wei Y
AD  - Department of Food Science and Engineering, College of Biological Sciences and 
      Technology, Beijing Forestry University, Beijing, China.
FAU - Zhang, Haiping
AU  - Zhang H
AD  - Department of Food Science and Engineering, College of Biological Sciences and 
      Technology, Beijing Forestry University, Beijing, China.
FAU - Sun, Ai-Dong
AU  - Sun AD
AUID- ORCID: 0000-0001-8854-8887
AD  - Department of Food Science and Engineering, College of Biological Sciences and 
      Technology, Beijing Forestry University, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180625
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
SB  - IM
MH  - Calcium-Transporting ATPases
MH  - Cell Membrane/chemistry/*ultrastructure
MH  - DNA, Fungal/*chemistry
MH  - Electric Stimulation
MH  - Electricity
MH  - Flow Cytometry
MH  - Food Preservation/*instrumentation/methods
MH  - Fungal Proteins/*chemistry
MH  - Membrane Potentials
MH  - Microarray Analysis
MH  - Molecular Conformation
MH  - Pichia/physiology/*ultrastructure
MH  - Protein Structure, Secondary
PMC - PMC6016922
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/06/26 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/06/25
CRDT- 2018/06/26 06:00
PHST- 2017/11/06 00:00 [received]
PHST- 2018/05/18 00:00 [accepted]
PHST- 2018/06/26 06:00 [entrez]
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/06/25 00:00 [pmc-release]
AID - PONE-D-17-39480 [pii]
AID - 10.1371/journal.pone.0198467 [doi]
PST - epublish
SO  - PLoS One. 2018 Jun 25;13(6):e0198467. doi: 10.1371/journal.pone.0198467. 
      eCollection 2018.