PMID- 29940130
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20220331
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 96
IP  - 6
DP  - 2018 Dec
TI  - MicroRNA-126 attenuates cell apoptosis by targeting TRAF7 in acute myeloid 
      leukemia cells.
PG  - 840-846
LID - 10.1139/bcb-2018-0017 [doi]
AB  - Acute myeloid leukemia (AML) has a 5-year survival rate of only about 30%-40% due 
      to the self-renewal and differentiation ability of leukemia stem-like cells 
      (LSCs). To address the potential for novel therapeutic targets in LSCs, we 
      investigated the roles of miRNA-126 and tumor necrosis factor receptor-associated 
      factor 7 (TRAF7) in AML. We used qRT-PCR and Western blot to investigate the 
      expression levels of miRNA-126 and TRAF7 in AML cell lines. Then, we uncovered 
      the effect of miRNA-126 on AML cell proliferation and apoptosis by MTT assay and 
      flow cytometric analysis, respectively. Furthermore, dual-luciferase assay and 
      Western blot were used to determine the target of miRNA-126 in AML and the 
      potential mechanism by which cell apoptosis is suppressed by miRNA-126. We found 
      that miRNA-126 was highly expressed in all of the AML cell lines, and that 
      inhibition of miRNA-126 significantly induced cell death through apoptosis. The 
      suppression of apoptosis in AML with high expression of miRNA-126 was caused by 
      down-regulating TRAF7, which blocked the c-FLIP pathway. The role of miRNA-126 in 
      AML makes it a potential therapeutic target to improve clinical outcomes for 
      patients with AML.
FAU - Ding, Qian
AU  - Ding Q
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
FAU - Wang, Qing
AU  - Wang Q
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
FAU - Ren, Yi
AU  - Ren Y
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
FAU - Zhu, Hong Qian
AU  - Zhu HQ
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
FAU - Huang, ZhuYun
AU  - Huang Z
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
AD  - Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, 
      P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (MIRN126 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (TRAF7 protein, human)
RN  - 0 (Tumor Necrosis Factor Receptor-Associated Peptides and Proteins)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Down-Regulation
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - MicroRNAs/*genetics
MH  - Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/*genetics
MH  - Up-Regulation
OTO - NOTNLM
OT  - TRAF7
OT  - acute myeloid leukemia cells
OT  - apoptose
OT  - apoptosis
OT  - cellules de leucemie myeloide aigue
OT  - miARN-126
OT  - miRNA-126
EDAT- 2018/06/26 06:00
MHDA- 2020/06/23 06:00
CRDT- 2018/06/26 06:00
PHST- 2018/06/26 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2018/06/26 06:00 [entrez]
AID - 10.1139/bcb-2018-0017 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2018 Dec;96(6):840-846. doi: 10.1139/bcb-2018-0017.