PMID- 29940854 OWN - NLM STAT- MEDLINE DCOM- 20190708 LR - 20190708 IS - 1471-2172 (Electronic) IS - 1471-2172 (Linking) VI - 19 IP - 1 DP - 2018 Jun 25 TI - Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection. PG - 19 LID - 10.1186/s12865-018-0258-8 [doi] LID - 19 AB - BACKGROUND: Control of Mycobacterium tuberculosis (Mtb) infection requires CD4(+) T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-cell immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial immune responses at early infection time points, but the kinetics of Ag presentation by these cells during these events are incompletely understood. RESULTS: In the present study, we evaluated in vivo dynamics of early Ag presentation by murine lymph-node (LN) DCs in response to Mycobacterium bovis bacillus Calmette-Guerin (BCG) Ag85A protein. Results showed that the early Ag-presenting activity of murine DCs induced by M. bovis BCG Ag85A protein in vivo was transient, appearing at 4 h and being barely detectable at 72 h. The transcription levels of CIITA, MHC II and the expression of MHC II molecule on the cell surface increased following BCG infection. Moreover, BCG was found to survive within the inguinal LN DC pool, representing a continuing source of mycobacterial Ag85A protein, with which LN DCs formed Ag85A peptide-MHCII complexes in vivo. CONCLUSIONS: Our results demonstrate that a decrease in Ag85A peptide production as a result of the inhibition of Ag processing to is largely responsible for the short duration of Ag presentation by LN DCs during BCG infection in vivo. FAU - Xu, Zhengzhong AU - Xu Z AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. FAU - Xia, Aihong AU - Xia A AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. FAU - Li, Xin AU - Li X AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. FAU - Zhu, Zhaocheng AU - Zhu Z AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. FAU - Shen, Yechi AU - Shen Y AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. FAU - Jin, Shanshan AU - Jin S AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. FAU - Lan, Tian AU - Lan T AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. FAU - Xie, Yuqing AU - Xie Y AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. FAU - Wu, Han AU - Wu H AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. FAU - Meng, Chuang AU - Meng C AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. FAU - Sun, Lin AU - Sun L AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. FAU - Yin, Yuelan AU - Yin Y AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. FAU - Chen, Xiang AU - Chen X AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, 225009, Jiangsu, China. chenxiang@yzu.edu.cn. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China. chenxiang@yzu.edu.cn. FAU - Jiao, Xinan AU - Jiao X AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou University, Yangzhou, China. jiao@yzu.edu.cn. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China. jiao@yzu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180625 PL - England TA - BMC Immunol JT - BMC immunology JID - 100966980 RN - 0 (Adjuvants, Immunologic) RN - 0 (Antigens, Bacterial) RN - 0 (BCG Vaccine) RN - 0 (Histocompatibility Antigens Class II) RN - 82115-62-6 (Interferon-gamma) RN - EC 2.3.- (Acyltransferases) RN - EC 2.3.1.- (antigen 85A, Mycobacterium tuberculosis) SB - IM MH - Acyltransferases/*immunology/metabolism MH - Adjuvants, Immunologic/administration & dosage MH - Animals MH - Antigen Presentation/*immunology MH - Antigens, Bacterial/*immunology/metabolism MH - BCG Vaccine/administration & dosage/immunology MH - Cell Survival/immunology MH - Dendritic Cells/*immunology/metabolism/microbiology MH - Histocompatibility Antigens Class II/immunology/metabolism MH - Interferon-gamma/immunology/metabolism MH - Lymph Nodes/*immunology/metabolism/microbiology MH - Mice, Inbred C57BL MH - Mycobacterium bovis/*immunology/physiology MH - Time Factors MH - Tuberculosis/*immunology/prevention & control/veterinary PMC - PMC6019797 OTO - NOTNLM OT - Ag-presenting activity OT - Dendritic cell OT - In vivo OT - M. bovis BCG OT - Major histocompatibility complex class II COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The mice were housed, handled, and immunized at our animal biosafety facilities, and all procedures were approved by the Institutional Animal Experimental Committee of Yangzhou University. All experiments were performed according to the national guidelines for animal welfare. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/06/27 06:00 MHDA- 2019/07/10 06:00 PMCR- 2018/06/25 CRDT- 2018/06/27 06:00 PHST- 2018/03/27 00:00 [received] PHST- 2018/06/18 00:00 [accepted] PHST- 2018/06/27 06:00 [entrez] PHST- 2018/06/27 06:00 [pubmed] PHST- 2019/07/10 06:00 [medline] PHST- 2018/06/25 00:00 [pmc-release] AID - 10.1186/s12865-018-0258-8 [pii] AID - 258 [pii] AID - 10.1186/s12865-018-0258-8 [doi] PST - epublish SO - BMC Immunol. 2018 Jun 25;19(1):19. doi: 10.1186/s12865-018-0258-8.