PMID- 29941257 OWN - NLM STAT- MEDLINE DCOM- 20180727 LR - 20180727 IS - 1001-0742 (Print) IS - 1001-0742 (Linking) VI - 69 DP - 2018 Jul TI - Preliminary investigation on cytotoxicity of fluorinated polymer nanoparticles. PG - 217-226 LID - S1001-0742(17)30828-8 [pii] LID - 10.1016/j.jes.2017.10.014 [doi] AB - As well-known persistent organic pollutants (POPs), organofluorine pollutants such as perfluorooctane sulfonate (PFOS) have been proven to be bioaccumulated and harmful to health. However, toxicological assessment of organofluorinated nanoparticles, which have emerged as a novel tool for biomedical and industrial applications, is lacking, to the best of our knowledge. To assess the biological effects and health risk of fluorinated nanoparticles, trifluoroethyl aryl ether-based fluorinated poly(methyl methacrylate) nanoparticles (PTFE-PMMA NPs) were synthesized with various fluorine contents (PTFE-PMMA-1 NPs 12.0wt.%, PTFE-PMMA-2 NPs 6.1wt.% and PTFE-PMMA-3 NPs 5.0wt.%), and their cytotoxicity was investigated in this study. The in vitro experimental results indicated that the cytotoxicity of PTFE-PMMA NPs was mild, and was closely related to their fluorine (F) contents and F-containing side chains. Specifically, the cytotoxicity of PTFE-PMMA NPs decreased with increasing F content and F-containing side chains. After exposure to PTFE-PMMA NPs at a sublethal dose (50mug/mL) for 24hr, the phospholipid bilayer was damaged, accompanied by increasing permeability of the cell membrane. Meanwhile, the intracellular accumulation of reactive oxygen species (ROS) occurred, resulting in the increase of DNA damage, cell cycle arrest and cell death. Overall, the PTFE-PMMA NPs were found to be relatively safe compared with typical engineered nanomaterials (ENMs), such as silver nanoparticles and graphene oxide, for biomedical and industrial applications. CI - Copyright (c) 2017. Published by Elsevier B.V. FAU - Wang, Xue AU - Wang X AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, China; Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Anhui Province, Hefei 230031, China. FAU - Cheng, Wenge AU - Cheng W AD - School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China; Key Laboratory of Organofluorine Chemistry and Laboratory of Polymer Materials, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China. FAU - Yang, Qiuyuan AU - Yang Q AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. FAU - Niu, Hongyun AU - Niu H AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. FAU - Liu, Qian AU - Liu Q AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. FAU - Liu, Yun AU - Liu Y AD - Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Anhui Province, Hefei 230031, China. FAU - Gao, Ming AU - Gao M AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. FAU - Xu, Ming AU - Xu M AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: mingxu@rcees.ac.cn. FAU - Xu, An AU - Xu A AD - Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences and Anhui Province, Hefei 230031, China. FAU - Liu, Sijin AU - Liu S AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Huang, Xiaoyu AU - Huang X AD - Key Laboratory of Organofluorine Chemistry and Laboratory of Polymer Materials, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address: xyhuang@sioc.ac.cn. FAU - Du, Yuguo AU - Du Y AD - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. LA - eng PT - Journal Article DEP - 20171107 PL - Netherlands TA - J Environ Sci (China) JT - Journal of environmental sciences (China) JID - 100967627 RN - 0 (Alkanesulfonic Acids) RN - 0 (Fluorocarbon Polymers) RN - 0 (Fluorocarbons) RN - 0 (Reactive Oxygen Species) RN - 9011-14-7 (Polymethyl Methacrylate) RN - 9H2MAI21CL (perfluorooctane sulfonic acid) SB - IM MH - Alkanesulfonic Acids/toxicity MH - Cell Death MH - Cell Survival/drug effects MH - DNA Damage MH - Fluorocarbon Polymers/*toxicity MH - Fluorocarbons/toxicity MH - Metal Nanoparticles/chemistry/*toxicity MH - Nanoparticles/*toxicity MH - Polymethyl Methacrylate/toxicity MH - Reactive Oxygen Species MH - *Toxicity Tests OTO - NOTNLM OT - Cytotoxicity OT - Fluorinated nanoparticles OT - Health risk OT - Oxidative stress OT - Polymer EDAT- 2018/06/27 06:00 MHDA- 2018/07/28 06:00 CRDT- 2018/06/27 06:00 PHST- 2017/03/19 00:00 [received] PHST- 2017/10/18 00:00 [revised] PHST- 2017/10/24 00:00 [accepted] PHST- 2018/06/27 06:00 [entrez] PHST- 2018/06/27 06:00 [pubmed] PHST- 2018/07/28 06:00 [medline] AID - S1001-0742(17)30828-8 [pii] AID - 10.1016/j.jes.2017.10.014 [doi] PST - ppublish SO - J Environ Sci (China). 2018 Jul;69:217-226. doi: 10.1016/j.jes.2017.10.014. Epub 2017 Nov 7.