PMID- 29941365
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20190114
IS  - 1879-0070 (Electronic)
IS  - 0732-8893 (Linking)
VI  - 92
IP  - 3
DP  - 2018 Nov
TI  - Comparison of clinical performance of commercial urine growth stabilization 
      products.
PG  - 179-182
LID - S0732-8893(18)30180-9 [pii]
LID - 10.1016/j.diagmicrobio.2018.05.023 [doi]
AB  - INTRODUCTION: Urine specimens for quantitative culture for the diagnosis of 
      urinary tract infection may be unreliable due to bacterial overgrowth within 4 h 
      after collection, at room temperature. Because specimen transportation may take 
      longer than 4 h, urine preservatives may reduce overgrowth. Further evidence is 
      needed to support a recommendation for use of preservative and to compare 
      preservative products. METHODS: Consecutive midstream urine specimens submitted 
      for culture were quantitatively cultured on receipt and then inoculated into 3 
      storage conditions [BD Urine Vacutainer (BD), Copan UriSwab (US), and 
      refrigeration, with a room temperature control] for 72 h, with quantitative 
      culture performed every 24 h. Odds ratio for significant growth interpretation 
      was reported. RESULTS: Ninety-five of 501 (19.0%) urine specimens demonstrated 
      significant growth. Within 24 h of storage, unpreserved urine at room temperature 
      demonstrated a significantly increased odds ratio for significant growth as 
      compared to preserved urine, and urine in refrigeration demonstrated similar odds 
      ratio for significant growth as compared to preserved. There was no significant 
      difference between the performance of US and BD. Over 48 and 72 h of storage, 
      odds ratio for significant growth further increased. CONCLUSIONS: Preservation 
      performed similarly to refrigeration. Preserved urine demonstrated a doubling in 
      odds ratio for significant growth after 24 h. This increase may negatively impact 
      antibiotic treatment decisions.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Daley, Peter
AU  - Daley P
AD  - Memorial University, St. John's, Canada. Electronic address: pkd336@mun.ca.
FAU - Gill, Yuvraj
AU  - Gill Y
AD  - Royal College of Surgeons, Ireland.
FAU - Midodzi, William
AU  - Midodzi W
AD  - Memorial University, St. John's, Canada.
LA  - eng
PT  - Journal Article
DEP - 20180601
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Bacterial Load
MH  - Humans
MH  - Odds Ratio
MH  - Preservation, Biological/*methods/*standards
MH  - Reagent Kits, Diagnostic/*standards
MH  - Specimen Handling/*methods/*standards
MH  - Time Factors
MH  - Urinary Tract Infections/*diagnosis/*microbiology
MH  - Urine/*microbiology
MH  - Urine Specimen Collection
OTO - NOTNLM
OT  - Preservative
OT  - Urine culture
EDAT- 2018/06/27 06:00
MHDA- 2019/01/15 06:00
CRDT- 2018/06/27 06:00
PHST- 2018/01/26 00:00 [received]
PHST- 2018/05/14 00:00 [revised]
PHST- 2018/05/24 00:00 [accepted]
PHST- 2018/06/27 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2018/06/27 06:00 [entrez]
AID - S0732-8893(18)30180-9 [pii]
AID - 10.1016/j.diagmicrobio.2018.05.023 [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 2018 Nov;92(3):179-182. doi: 
      10.1016/j.diagmicrobio.2018.05.023. Epub 2018 Jun 1.