PMID- 29943387
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1463-1326 (Electronic)
IS  - 1462-8902 (Linking)
VI  - 20
IP  - 11
DP  - 2018 Nov
TI  - Plasma levels of free fatty acids correlate with type 2 diabetes mellitus.
PG  - 2661-2669
LID - 10.1111/dom.13449 [doi]
AB  - AIMS: Free fatty acids (FFA) mediate adverse metabolic effects such as 
      downregulated carbohydrate metabolisms, providing causal links between obesity 
      and the development of type 2 diabetes mellitus (T2DM). Here, we investigated the 
      plasma concentrations of FFA alone and in combination with protein glycation as 
      potential diagnostic and prognostic biomarkers of T2DM. MATERIALS AND METHODS: 
      EDTA-plasma obtained from 48 newly diagnosed male T2DM patients, 48 long-term 
      controlled (24 male and 24 female) T2DM patients, 20 prediabetic male T2DM 
      patients and two age-matched control cohorts (48 non-diabetic (ND) men; 24 male 
      and 24 female ND partipants) were analysed for a set of clinical parameters 
      including FFA. Glycation sites were quantified after tryptic digestion using 
      tandem mass spectrometry. RESULTS: Median plasma concentrations of FFA were 
      almost three-fold higher in samples obtained from newly diagnosed (long-term 
      controlled) T2DM patients than in those obtained from the control group, 
      providing diagnostic sensitivity (SN) of 92% (85%) and specificity (SP) of 90% 
      (88%). When combined with the glycation level of lysine-141 of haptoglobin, 
      diagnostic accuracy improved further for newly diagnosed (SN, 94%; SP 96%) and 
      long-term controlled (SN, 85%; SP, 94%) T2DM patients (HbA1c: SN, 88%; SP, 96%). 
      A prospective pilot study evaluating the prognostic value revealed initially low 
      FFA levels for pre-diabetic patients that increased in the following four years 
      in patients whose prediabetic state worsened or who developed T2DM. CONCLUSIONS: 
      FFA levels are elevated in newly diagnosed and long-term controlled T2DM 
      patients, providing high diagnostic accuracy of 87% and 91%, respectively, which 
      improved further when combined with the glycation degree of lysine-141 in 
      haptoglobin. Additionally, FFA showed higher mean fold-changes than HbA1c or FPG 
      in subjects developing T2DM, indicating higher sensitivity towards the 
      progression of the disease.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Spiller, Sandro
AU  - Spiller S
AD  - Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, 
      Universitat Leipzig, Leipzig, Germany.
AD  - Center for Biotechnology and Biomedicine (BBZ), Universitat Leipzig, Leipzig, 
      Germany.
FAU - Bluher, Matthias
AU  - Bluher M
AD  - University Hospital Leipzig, Department of Internal Medicine, Endocrinology and 
      Nephrology, Universitat Leipzig, Leipzig, Germany.
FAU - Hoffmann, Ralf
AU  - Hoffmann R
AUID- ORCID: 0000-0001-9932-5646
AD  - Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, 
      Universitat Leipzig, Leipzig, Germany.
AD  - Center for Biotechnology and Biomedicine (BBZ), Universitat Leipzig, Leipzig, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180730
PL  - England
TA  - Diabetes Obes Metab
JT  - Diabetes, obesity & metabolism
JID - 100883645
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (HP protein, human)
RN  - 0 (Haptoglobins)
SB  - IM
MH  - Adult
MH  - Biomarkers/*blood
MH  - Blood Glucose/metabolism
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*blood/*diagnosis
MH  - Fatty Acids, Nonesterified/*blood
MH  - Female
MH  - Haptoglobins/metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pilot Projects
MH  - Prediabetic State/blood/diagnosis
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - biomarker
OT  - free fatty acids (FFA)
OT  - glycation sites
OT  - non-esterified fatty acids (NEFA)
OT  - plasma proteins
OT  - protein glycation
OT  - type 2 diabetes mellitus
EDAT- 2018/06/27 06:00
MHDA- 2019/04/16 06:00
CRDT- 2018/06/27 06:00
PHST- 2018/01/31 00:00 [received]
PHST- 2018/06/21 00:00 [revised]
PHST- 2018/06/22 00:00 [accepted]
PHST- 2018/06/27 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2018/06/27 06:00 [entrez]
AID - 10.1111/dom.13449 [doi]
PST - ppublish
SO  - Diabetes Obes Metab. 2018 Nov;20(11):2661-2669. doi: 10.1111/dom.13449. Epub 2018 
      Jul 30.