PMID- 29943843
OWN - NLM
STAT- MEDLINE
DCOM- 20191008
LR  - 20221207
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 9
DP  - 2018 Sep
TI  - Generation of tolerogenic dendritic cells using Lactobacillus rhamnosus and 
      Lactobacillus delbrueckii as tolerogenic probiotics.
PG  - 7865-7872
LID - 10.1002/jcb.27203 [doi]
AB  - Systemic lupus erythematosus (SLE) concurs with excessive uncontrolled 
      inflammatory immune responses that lead to the loss of immune tolerance. 
      Dendritic cells (DCs) are important and determinant immune cells that regulate 
      immune responses. Tolerogenic DCs with regulatory markers and cytokines could 
      induce regulatory immune cells and responses. Tolerogenic probiotics are capable 
      of producing regulatory DCs from monocytes in in vitro conditions. The purpose of 
      this study was to evaluate the effect of Lactobacillus delbrueckii and 
      Lactobacillus rhamnosus on the production of DCs in an in vitro condition. 
      Peripheral blood mononuclear cells were isolated from the healthy and SLE donors. 
      Monocytes were cultured with optimized concentrations of granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) to produce immature 
      DCs (IDCs). An IDC uptake assay was performed, and IDCs of healthy and SLE donors 
      were divided into three subgroups following 48 hours of treatment with GM-CSF and 
      IL-4, along with L. delbrueckii, L. rhamnosus, and mixed probiotics for the 
      production of tolerogenic DCs. The surface expression of Human Leukocyte 
      Antigen-antigen D Related (HLA-DR), CD86, CD80, CD83, CD1a, and CD14 was analyzed 
      using flow cytometry, and the gene expression levels of indoleamine 
      2,3-dioxygenase (IDO), IL-10, and IL-12 were measured using real-time polymerase 
      chain reaction. We observed significantly reduced expression of costimulatory 
      molecules and other surface markers in the probiotic-induced mature DCs (MDCs) in 
      both healthy and SLE donor groups in comparison with lipopolysaccharide 
      (LPS)-induced MDCs. In addition, the expression of IDO and IL-10 increased, 
      whereas IL-12 decreased significantly in probiotic-induced MDCs compared with 
      LPS-induced MDCs. IDCs and especially mature tolerogenic DC of SLE patients 
      highly expressed IDO. The results of the current study suggested that live 
      probiotics could modify properties of DCs to modulatory cells, which might 
      contribute to the induction of tolerance and renovation of immune hemostasis.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Esmaeili, Seyed-Alireza
AU  - Esmaeili SA
AD  - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
AD  - Immunology Department, Faculty of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
AD  - Student Research Committee, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
FAU - Mahmoudi, Mahmoud
AU  - Mahmoudi M
AD  - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
AD  - Immunology Department, Faculty of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Rezaieyazdi, Zahra
AU  - Rezaieyazdi Z
AD  - Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
FAU - Sahebari, Maryam
AU  - Sahebari M
AD  - Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
FAU - Tabasi, Nafiseh
AU  - Tabasi N
AD  - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
FAU - Sahebkar, Amirhossein
AU  - Sahebkar A
AUID- ORCID: 0000-0002-8656-1444
AD  - Neurogenic inflammation Research Center, Mashhad University of Medical Sciences, 
      Mashhad, Iran.
AD  - Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
AD  - School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
FAU - Rastin, Maryam
AU  - Rastin M
AD  - Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
AD  - Immunology Department, Faculty of Medicine, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180626
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Cytokines)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Cytokines/genetics/metabolism
MH  - Dendritic Cells/*cytology/immunology/microbiology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics/metabolism
MH  - Interleukin-4/pharmacology
MH  - Lactobacillus delbrueckii/immunology/*physiology
MH  - Lacticaseibacillus rhamnosus/immunology/*physiology
MH  - Lupus Erythematosus, Systemic/immunology/*microbiology
MH  - Male
MH  - Monocytes/*cytology/drug effects/immunology/microbiology
MH  - Probiotics
OTO - NOTNLM
OT  - Lactobacillus
OT  - dendritic cells
OT  - probiotics
OT  - systemic lupus erythematosus
EDAT- 2018/06/27 06:00
MHDA- 2019/10/09 06:00
CRDT- 2018/06/27 06:00
PHST- 2018/02/27 00:00 [received]
PHST- 2018/05/24 00:00 [accepted]
PHST- 2018/06/27 06:00 [pubmed]
PHST- 2019/10/09 06:00 [medline]
PHST- 2018/06/27 06:00 [entrez]
AID - 10.1002/jcb.27203 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Sep;119(9):7865-7872. doi: 10.1002/jcb.27203. Epub 2018 Jun 
      26.