PMID- 29944202
OWN - NLM
STAT- MEDLINE
DCOM- 20190411
LR  - 20210930
IS  - 1934-9300 (Electronic)
IS  - 1934-9297 (Print)
IS  - 1934-9297 (Linking)
VI  - 85
IP  - 1
DP  - 2018 Jul
TI  - Multiphoton Intravital Calcium Imaging.
PG  - e40
LID - 10.1002/cpcy.40 [doi]
AB  - Multiphoton intravital calcium imaging is a powerful technique that enables 
      high-resolution longitudinal monitoring of cellular and subcellular activity 
      hundreds of microns deep in the living organism. This unit addresses the 
      application of 2-photon microscopy to imaging of genetically encoded calcium 
      indicators (GECIs) in the mouse brain. The protocols in this unit enable 
      real-time intravital imaging of intracellular calcium concentration 
      simultaneously in hundreds of neurons, or at the resolution of single synapses, 
      as mice respond to sensory stimuli or perform behavioral tasks. Protocols are 
      presented for implantation of a cranial imaging window to provide optical access 
      to the brain and for 2-photon image acquisition. Protocols for implantation of 
      both open skull and thinned skull windows for single or multi-session imaging are 
      described. (c) 2018 by John Wiley & Sons, Inc.
CI  - (c) 2018 John Wiley & Sons, Inc.
FAU - Cheetham, Claire E J
AU  - Cheetham CEJ
AUID- ORCID: 0000-0002-4038-1501
AD  - Department of Neurobiology, University of Pittsburgh, Pittsburgh, Pennsylvania.
LA  - eng
GR  - R01 DC018516/DC/NIDCD NIH HHS/United States
GR  - R03 DC014788/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180626
PL  - United States
TA  - Curr Protoc Cytom
JT  - Current protocols in cytometry
JID - 100899351
SB  - IM
MH  - Animals
MH  - *Brain/cytology/physiology
MH  - Calcium Signaling/*physiology
MH  - Intravital Microscopy/instrumentation/*methods
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Fluorescence, Multiphoton/instrumentation/*methods
MH  - Synapses/*physiology
PMC - PMC6039231
MID - NIHMS954956
OTO - NOTNLM
OT  - 2-photon microscopy
OT  - brain
OT  - cranial window
OT  - genetically-encoded calcium indicator
OT  - in vivo imaging
OT  - intravital imaging
OT  - multiphoton imaging
EDAT- 2018/06/27 06:00
MHDA- 2019/04/12 06:00
PMCR- 2019/07/01
CRDT- 2018/06/27 06:00
PHST- 2018/06/27 06:00 [pubmed]
PHST- 2019/04/12 06:00 [medline]
PHST- 2018/06/27 06:00 [entrez]
PHST- 2019/07/01 00:00 [pmc-release]
AID - 10.1002/cpcy.40 [doi]
PST - ppublish
SO  - Curr Protoc Cytom. 2018 Jul;85(1):e40. doi: 10.1002/cpcy.40. Epub 2018 Jun 26.