PMID- 29946744 OWN - NLM STAT- MEDLINE DCOM- 20181114 LR - 20181114 IS - 1615-7605 (Electronic) IS - 1615-7591 (Linking) VI - 41 IP - 10 DP - 2018 Oct TI - Bio-succinic acid production from coffee husk treated with thermochemical and fungal hydrolysis. PG - 1461-1470 LID - 10.1007/s00449-018-1974-4 [doi] AB - Coffee husk (CH), a waste obtained from processing of coffee cherries via dry method, causes serious environmental problems. In this study, strategies were designed to utilize CH for succinic acid (SA) production. Three different CH hydrolysis methods: thermal, thermochemical and crude enzymes obtained by solid state fermentation of Aspergillus niger and Trichoderma reesei, were evaluated to generate fermentable feedstock for SA production using Actinobacillus succinogenes. The feasibility of these pretreatment methods was investigated. Accordingly, thermochemical hydrolysis using H(2)SO(4) at 121 degrees C for 30 min, appeared the most effective method for CH hydrolysis, producing 24.4 g/L of reducing sugars (RS). Finally, 19.3 g/L of SA with yield and productivity of 0.95 g SA/g RS and 0.54 g/L/h, respectively, were obtained using CH hydrolysate. The current study revealed an alternative way of utilization coffee waste for value addition while mitigating environmental problems caused by its disposal. FAU - Dessie, Wubliker AU - Dessie W AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. AD - Department of Biology, College of Natural and Computational Science, Mizan-Tepi University, P.O. Box 121, Tepi, Ethiopia. FAU - Zhu, Junru AU - Zhu J AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. FAU - Xin, Fengxue AU - Xin F AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. FAU - Zhang, Wenming AU - Zhang W AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. AD - Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, People's Republic of China. FAU - Jiang, Youming AU - Jiang Y AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. FAU - Wu, Hao AU - Wu H AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. FAU - Ma, Jiangfeng AU - Ma J AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. FAU - Jiang, Min AU - Jiang M AD - State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816, People's Republic of China. bioengine@njtech.edu.cn. AD - Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, People's Republic of China. bioengine@njtech.edu.cn. LA - eng GR - KL16-08/The Project of State Key Laboratory of Materials-Oriented Chemical Engineering/ GR - PPZY2015B155/Top-notch Academic Programs Project of Jiangsu Higher Education Institutions/ GR - TAPP./Top-notch Academic Programs Project of Jiangsu Higher Education Institutions/ GR - BE2016389/The Key Science and Technology Project of Jiangsu Province/ GR - No. 21706125/The National Natural Science Foundation of China/ GR - No. 21727818/The National Natural Science Foundation of China/ GR - No. 21706124/The National Natural Science Foundation of China/ GR - No. 3170/The National Natural Science Foundation of China/ PT - Journal Article DEP - 20180626 PL - Germany TA - Bioprocess Biosyst Eng JT - Bioprocess and biosystems engineering JID - 101088505 RN - 0 (Coffee) RN - 0 (Sulfuric Acids) RN - AB6MNQ6J6L (Succinic Acid) RN - O40UQP6WCF (sulfuric acid) SB - IM MH - Actinobacillus/*growth & development MH - Aspergillus niger/*growth & development MH - Coffee/*chemistry MH - Hydrolysis MH - Succinic Acid/*metabolism MH - Sulfuric Acids/*chemistry MH - Trichoderma/*growth & development OTO - NOTNLM OT - Actinobacillus succinogenes OT - Coffee husk OT - Hydrolysis OT - Pretreatment OT - Succinic acid EDAT- 2018/06/28 06:00 MHDA- 2018/11/15 06:00 CRDT- 2018/06/28 06:00 PHST- 2018/05/02 00:00 [received] PHST- 2018/06/22 00:00 [accepted] PHST- 2018/06/28 06:00 [pubmed] PHST- 2018/11/15 06:00 [medline] PHST- 2018/06/28 06:00 [entrez] AID - 10.1007/s00449-018-1974-4 [pii] AID - 10.1007/s00449-018-1974-4 [doi] PST - ppublish SO - Bioprocess Biosyst Eng. 2018 Oct;41(10):1461-1470. doi: 10.1007/s00449-018-1974-4. Epub 2018 Jun 26.