PMID- 29947220
OWN - NLM
STAT- MEDLINE
DCOM- 20190826
LR  - 20190826
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 17
IP  - 8
DP  - 2018 Aug 3
TI  - Human Metabolome Changes after a Single Dose of 3,4-Methylenedioxymethamphetamine 
      (MDMA) with Special Focus on Steroid Metabolism and Inflammation Processes.
PG  - 2900-2907
LID - 10.1021/acs.jproteome.8b00438 [doi]
AB  - The intake of 3,4-methylenedioxymethamphetamine (MDMA) is known to increase 
      several endogenous substances involved in steroid and inflammation pathways. 
      Untargeted metabolomics screening approaches can determine biochemical changes 
      after drug exposure and can reveal new pathways, which might be involved in the 
      pharmacology and toxicology of a drug of abuse. We analyzed plasma samples from a 
      placebo-controlled crossover study of a single intake of MDMA. Plasma samples 
      from a time point before and three time points after the intake of a single dose 
      of 125 mg MDMA were screened for changes of endogenous metabolites. An untargeted 
      metabolomics approach on a high-resolution quadrupole time-of-flight mass 
      spectrometer coupled to liquid chromatography with two different chromatographic 
      systems (reversed-phase and hydrophobic interaction liquid chromatography) was 
      applied. Over 10 000 features of the human metabolome were detected. Hence, 28 
      metabolites were identified, which showed significant changes after 
      administration of MDMA compared with placebo. The analysis revealed an 
      upregulation of cortisol and pregnenolone sulfate 4 h after MDMA intake, 
      suggesting increased stress and serotonergic activity. Furthermore, calcitriol 
      levels were decreased after the intake of MDMA. Calcitriol is involved in the 
      upregulation of trophic factors, which have protective effects on brain dopamine 
      neurons. The inflammation mediators hydroxyeicosatetraenoic acid, 
      dihydroxyeicosatetraenoic acid, and octadecadienoic acid were found to be 
      upregulated after the intake of MDMA compared with placebo, which suggested a 
      stimulation of inflammation pathways.
FAU - Boxler, Martina I
AU  - Boxler MI
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine , University of Zurich , 8057 Zurich , Switzerland.
FAU - Streun, Gabriel L
AU  - Streun GL
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine , University of Zurich , 8057 Zurich , Switzerland.
FAU - Liechti, Matthias E
AU  - Liechti ME
AD  - Psychopharmacology Research, Division of Clinical Pharmacology and Toxicology, 
      Department of Biomedicine, Department of Clinical Research , University Hospital 
      Basel, University of Basel , 4031 Basel , Switzerland.
FAU - Schmid, Yasmin
AU  - Schmid Y
AD  - Psychopharmacology Research, Division of Clinical Pharmacology and Toxicology, 
      Department of Biomedicine, Department of Clinical Research , University Hospital 
      Basel, University of Basel , 4031 Basel , Switzerland.
FAU - Kraemer, Thomas
AU  - Kraemer T
AUID- ORCID: 0000-0002-3283-606X
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine , University of Zurich , 8057 Zurich , Switzerland.
FAU - Steuer, Andrea E
AU  - Steuer AE
AUID- ORCID: 0000-0001-8983-2353
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine , University of Zurich , 8057 Zurich , Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180713
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Inflammation Mediators)
RN  - 0 (Steroids)
RN  - 73R90F7MQ8 (Pregnenolone)
RN  - FXC9231JVH (Calcitriol)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - WI4X0X7BPJ (Hydrocortisone)
MH  - Blood Specimen Collection
MH  - Calcitriol/metabolism
MH  - Cross-Over Studies
MH  - Humans
MH  - Hydrocortisone/metabolism
MH  - Inflammation/*chemically induced
MH  - Inflammation Mediators/metabolism
MH  - Mass Spectrometry
MH  - Metabolome/*drug effects
MH  - Metabolomics/methods
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Pregnenolone/metabolism
MH  - Steroids/*metabolism
MH  - Time Factors
OTO - NOTNLM
OT  - 3,4-methylenedioxymethamphetamine (MDMA)
OT  - calcitriol
OT  - ecstasy
OT  - inflammation
OT  - placebo-controlled
OT  - pregnenolone sulfate
OT  - untargeted metabolomics
EDAT- 2018/06/28 06:00
MHDA- 2019/08/27 06:00
CRDT- 2018/06/28 06:00
PHST- 2018/06/28 06:00 [pubmed]
PHST- 2019/08/27 06:00 [medline]
PHST- 2018/06/28 06:00 [entrez]
AID - 10.1021/acs.jproteome.8b00438 [doi]
PST - ppublish
SO  - J Proteome Res. 2018 Aug 3;17(8):2900-2907. doi: 10.1021/acs.jproteome.8b00438. 
      Epub 2018 Jul 13.