PMID- 29947816 OWN - NLM STAT- MEDLINE DCOM- 20180822 LR - 20220409 IS - 1432-2242 (Electronic) IS - 0040-5752 (Linking) VI - 131 IP - 9 DP - 2018 Sep TI - Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high-resolution multiplex oligonucleotide FISH. PG - 1967-1986 LID - 10.1007/s00122-018-3126-2 [doi] AB - High-resolution multiplex oligonucleotide FISH revealed the frequent occurrence of structural chromosomal rearrangements and polymorphisms in widely grown wheat cultivars and their founders. Over 2000 wheat cultivars including 19 founders were released and grown in China from 1949 to 2000. To understand the impact of breeding selection on chromosome structural variations, high-resolution karyotypes of Chinese Spring (CS) and 373 Chinese cultivars were developed and compared by FISH (fluorescence in situ hybridization) using an oligonucleotide multiplex probe based on repeat sequences. Among them, 148 (39.7%) accessions carried 14 structural rearrangements including three single translocations (designated as T), eight reciprocal translocations (RT), one pericentric inversion (perInv), and two combined variations having both the deletion and single translocations. Five rearrangements were traced to eight founders, including perInv 6B detected in 57 cultivars originating from Funo, Abbondanza, and Fan 6, T 1RS∙1BL in 47 cultivars derived from the Lovrin series, RT 4AS∙4AL-1DS/1DL∙1DS-4AL in 31 varieties from Mazhamai and Bima 4, RT 1RS∙7DL/7DS∙1BL in three cultivars was from Aimengniu, and RT 5BS∙5BL-5DL/5DS∙5DL-5BL was only detected in Youzimai. In addition to structural rearrangements, 167 polymorphic chromosome blocks (defined as unique signal patterns of oligonucleotide repeat probes distributed within chromosomes) were identified, and 59 were present in one or more founders. Some specific types were present at high frequencies indicating selective blocks in Chinese wheat varieties. All cultivars and CS were clustered into four groups and 15 subgroups at chromosome level. Common block patterns occurred in the same subgroup. Origin, geographic distribution, probable adaptation to specific environments, and potential use of these chromosomal rearrangements and blocks are discussed. FAU - Huang, Xinyi AU - Huang X AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Zhu, Minqiu AU - Zhu M AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Zhuang, Lifang AU - Zhuang L AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Zhang, Siyu AU - Zhang S AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Wang, Junjuan AU - Wang J AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Chen, Xuejun AU - Chen X AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Wang, Danrui AU - Wang D AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Chen, Jianyong AU - Chen J AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Bao, Yinguang AU - Bao Y AD - State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian, 271018, China. FAU - Guo, Jie AU - Guo J AD - College of Agronomy, Shanxi Agricultural University, Taigu, 030801, Shanxi, China. FAU - Zhang, Jinlong AU - Zhang J AD - Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China. FAU - Feng, Yigao AU - Feng Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Chu, Chenggen AU - Chu C AD - Texas A&M Agrilife Research, Amarillo, TX, 79106, USA. FAU - Du, Pei AU - Du P AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002, China. FAU - Qi, Zengjun AU - Qi Z AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. zjqi@njau.edu.cn. FAU - Wang, Honggang AU - Wang H AD - State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian, 271018, China. hgwang@sdau.edu.cn. FAU - Chen, Peidu AU - Chen P AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. LA - eng GR - 31671681/National Natural Science Foundation of China/ PT - Journal Article DEP - 20180615 PL - Germany TA - Theor Appl Genet JT - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JID - 0145600 RN - 0 (Oligonucleotides) SB - IM MH - China MH - *Chromosome Inversion MH - Chromosomes, Plant/genetics MH - In Situ Hybridization, Fluorescence MH - Karyotype MH - Oligonucleotides MH - *Polymorphism, Genetic MH - *Translocation, Genetic MH - Triticum/*genetics EDAT- 2018/06/28 06:00 MHDA- 2018/08/23 06:00 CRDT- 2018/06/28 06:00 PHST- 2018/04/03 00:00 [received] PHST- 2018/06/01 00:00 [accepted] PHST- 2018/06/28 06:00 [pubmed] PHST- 2018/08/23 06:00 [medline] PHST- 2018/06/28 06:00 [entrez] AID - 10.1007/s00122-018-3126-2 [pii] AID - 10.1007/s00122-018-3126-2 [doi] PST - ppublish SO - Theor Appl Genet. 2018 Sep;131(9):1967-1986. doi: 10.1007/s00122-018-3126-2. Epub 2018 Jun 15.