PMID- 29947892
OWN - NLM
STAT- MEDLINE
DCOM- 20191028
LR  - 20231213
IS  - 1432-0738 (Electronic)
IS  - 0340-5761 (Linking)
VI  - 92
IP  - 8
DP  - 2018 Aug
TI  - Perinatal exposure to a glyphosate-based herbicide impairs female reproductive 
      outcomes and induces second-generation adverse effects in Wistar rats.
PG  - 2629-2643
LID - 10.1007/s00204-018-2236-6 [doi]
AB  - Glyphosate-based herbicides (GBHs) are the most globally used herbicides raising 
      the risk of environmental exposition. Here, we investigated whether perinatal 
      exposure to low doses of a GBH alters the female reproductive performance, and/or 
      induced second-generation effects related to congenital anomalies or growth 
      alterations. Pregnant rats (F0) received a GBH through food, in a dose of 2 mg 
      (GBH-LD: GBH-low dose group) or 200 mg (GBH-HD: GBH-high dose group) of 
      glyphosate/kg bw/day from gestational day (GD) 9 until weaning. Body weight gain 
      and vaginal canal-opening of F1 females were recorded. Sexually mature F1 females 
      were mated to evaluate their reproductive performance by assessing the pregnancy 
      rate, and on GD19, the number of corpora lutea, the implantation sites (IS) and 
      resorption sites. To analyze second-generation effects on F2 offspring, we 
      analyzed the fetal morphology on GD19, and assessed the fetal length and weight, 
      and the placental weight. GBH exposure neither altered the body weight gain of F1 
      females, nor vaginal opening onset. Although all GBH-exposed F1 rats became 
      pregnant, a lower number of IS was detected. F2 offspring from both GBH groups 
      showed delayed growth, evidenced by lower fetal weight and length, associated 
      with a higher incidence of small for gestational age fetuses. In addition, higher 
      placental weight and placental index were found in F2 offspring from GBH-HD dams. 
      Surprisingly, structural congenital anomalies (conjoined fetuses and abnormally 
      developed limbs) were detected in the F2 offspring from GBH-HD group. In 
      conclusion, perinatal exposure to low doses of a GBH impaired female reproductive 
      performance and induced fetal growth retardation and structural congenital 
      anomalies in F2 offspring.
FAU - Milesi, Maria M
AU  - Milesi MM
AD  - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y 
      Ciencias Biologicas, Universidad Nacional del Litoral (UNL)-Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Casilla de Correo 242, 3000, 
      Santa Fe, Argentina. mmilesi@fbcb.unl.edu.ar.
FAU - Lorenz, Virginia
AU  - Lorenz V
AD  - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y 
      Ciencias Biologicas, Universidad Nacional del Litoral (UNL)-Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Casilla de Correo 242, 3000, 
      Santa Fe, Argentina.
FAU - Pacini, Guillermina
AU  - Pacini G
AD  - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y 
      Ciencias Biologicas, Universidad Nacional del Litoral (UNL)-Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Casilla de Correo 242, 3000, 
      Santa Fe, Argentina.
FAU - Repetti, Maria R
AU  - Repetti MR
AD  - Programa de Investigacion y Analisis de Residuos y Contaminantes Quimicos 
      (PRINARC), Facultad de Ingenieria Quimica, UNL, Santa Fe, Argentina.
FAU - Demonte, Luisina D
AU  - Demonte LD
AD  - Programa de Investigacion y Analisis de Residuos y Contaminantes Quimicos 
      (PRINARC), Facultad de Ingenieria Quimica, UNL, Santa Fe, Argentina.
FAU - Varayoud, Jorgelina
AU  - Varayoud J
AD  - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y 
      Ciencias Biologicas, Universidad Nacional del Litoral (UNL)-Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Casilla de Correo 242, 3000, 
      Santa Fe, Argentina.
FAU - Luque, Enrique H
AU  - Luque EH
AD  - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y 
      Ciencias Biologicas, Universidad Nacional del Litoral (UNL)-Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas (CONICET), Casilla de Correo 242, 3000, 
      Santa Fe, Argentina.
LA  - eng
PT  - Journal Article
DEP - 20180609
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Herbicides)
RN  - 0 (Isoxazoles)
RN  - 0 (Tetrazoles)
RN  - J090588E6X 
      (2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid)
RN  - TE7660XO1C (Glycine)
SB  - IM
CIN - Arch Toxicol. 2019 Jan;93(1):207. PMID: 30456485
CIN - Arch Toxicol. 2019 Mar;93(3):831-832. PMID: 30725131
CIN - Arch Toxicol. 2019 Dec;93(12):3635-3638. PMID: 31720698
CIN - Arch Toxicol. 2020 Jan;94(1):351-352. PMID: 31853558
MH  - Animals
MH  - Body Weight/drug effects
MH  - Female
MH  - Fetal Growth Retardation/chemically induced
MH  - Glycine/administration & dosage/*analogs & derivatives/toxicity
MH  - Herbicides/toxicity
MH  - Isoxazoles/blood
MH  - Lactation
MH  - Male
MH  - Placenta/drug effects/pathology
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - Prenatal Exposure Delayed Effects/*chemically induced
MH  - Puberty/drug effects/physiology
MH  - Rats, Wistar
MH  - Reproduction/*drug effects/physiology
MH  - Tetrazoles/blood
MH  - Glyphosate
OTO - NOTNLM
OT  - Feto-placental parameters
OT  - Glyphosate-based herbicide
OT  - Reproductive performance
OT  - Uterus
EDAT- 2018/06/28 06:00
MHDA- 2019/10/29 06:00
CRDT- 2018/06/28 06:00
PHST- 2018/02/15 00:00 [received]
PHST- 2018/06/04 00:00 [accepted]
PHST- 2018/06/28 06:00 [pubmed]
PHST- 2019/10/29 06:00 [medline]
PHST- 2018/06/28 06:00 [entrez]
AID - 10.1007/s00204-018-2236-6 [pii]
AID - 10.1007/s00204-018-2236-6 [doi]
PST - ppublish
SO  - Arch Toxicol. 2018 Aug;92(8):2629-2643. doi: 10.1007/s00204-018-2236-6. Epub 2018 
      Jun 9.