PMID- 29949786 OWN - NLM STAT- MEDLINE DCOM- 20180815 LR - 20220409 IS - 1421-9778 (Electronic) IS - 1015-8987 (Linking) VI - 47 IP - 4 DP - 2018 TI - Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells. PG - 1696-1710 LID - 10.1159/000490992 [doi] AB - BACKGROUND/AIMS: CD133+CD44+ cancer stem cells previously isolated from laryngeal squamous cell carcinoma (LSCC) cell lines showed strong malignancy and tumorigenicity. However, the molecular mechanism underlying the enhanced malignancy remained unclear. METHODS: Cell proliferation assay, spheroid-formation experiment, RNA sequencing (RNA-seq), miRNA-seq, bioinformatic analysis, quantitative real-time PCR, migration assay, invasion assay, and luciferase reporter assay were used to identify differentially expressed mRNAs, lncRNAs, circRNAs and miRNAs, construct transcription regulatory network, and investigate functional roles and mechanism of circRNA in CD133+CD44+ laryngeal cancer stem cells. RESULTS: Differentially expressed genes in TDP cells were mainly enriched in the biological processes of cell differentiation, regulation of autophagy, negative regulation of cell death, regulation of cell growth, response to hypoxia, telomere maintenance, cellular response to gamma radiation, and regulation of apoptotic signaling, which are closely related to the malignant features of tumor cells. We constructed the regulatory network of differentially expressed circRNAs, miRNAs and mRNAs. qPCR findings for the expression of key genes in the network were consistent with the sequencing data. Moreover, our data revealed that circRNA hg19_circ_0005033 promotes proliferation, migration, invasion, and chemotherapy resistance of laryngeal cancer stem cells. CONCLUSIONS: This study provides potential biomarkers and targets for LSCC diagnosis and therapy, and provide important evidences for the heterogeneity of LSCC cells at the transcription level. CI - (c) 2018 The Author(s). Published by S. Karger AG, Basel. FAU - Wu, Yongyan AU - Wu Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Zhang, Yuliang AU - Zhang Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Niu, Min AU - Niu M AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Shi, Yong AU - Shi Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Liu, Hongliang AU - Liu H AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Yang, Dongli AU - Yang D AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Li, Fei AU - Li F AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Lu, Yan AU - Lu Y AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Jinzhou Medical University, Jinzhou, China. FAU - Bo, Yunfeng AU - Bo Y AD - Department of Pathology, Shanxi Cancer Hospital, Shanxi Medical University, Taiyuan, China. FAU - Zhang, Ruiping AU - Zhang R AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. AD - Department of MRI & CT, Shanxi Cancer Hospital, Shanxi Medical University, Taiyuan, China. FAU - Li, Zhenyu AU - Li Z AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. AD - Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China. FAU - Luo, Hongjie AU - Luo H AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Cui, Jiajia AU - Cui J AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Sang, Jiangwei AU - Sang J AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Xiang, Caixia AU - Xiang C AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Gao, Wei AU - Gao W AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. FAU - Wen, Shuxin AU - Wen S AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Taiyuan, China. AD - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Taiyuan, China. LA - eng PT - Journal Article DEP - 20180627 PL - Germany TA - Cell Physiol Biochem JT - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JID - 9113221 RN - 0 (AC133 Antigen) RN - 0 (Antigens, CD) RN - 0 (Biomarkers, Tumor) RN - 0 (Cadherins) RN - 0 (Neoplasm Proteins) RN - 0 (PROM1 protein, human) RN - 0 (cadherin 5) SB - IM EIN - Cell Physiol Biochem. 2020;54(4):792. PMID: 32853501 MH - AC133 Antigen/*metabolism MH - Antigens, CD/*metabolism MH - Biomarkers, Tumor/*metabolism MH - Cadherins/*metabolism MH - Carcinoma, Squamous Cell/diagnosis/*metabolism/pathology MH - Cell Line, Tumor MH - *Gene Expression Profiling MH - Humans MH - Laryngeal Neoplasms/*metabolism MH - Neoplasm Proteins/*metabolism MH - Neoplastic Stem Cells/*metabolism/pathology OTO - NOTNLM OT - CD133+CD44+ OT - Cancer stem cells OT - Circular RNA OT - Laryngeal squamous cell carcinoma OT - Whole transcriptome analysis EDAT- 2018/06/28 06:00 MHDA- 2018/08/16 06:00 CRDT- 2018/06/28 06:00 PHST- 2018/01/15 00:00 [received] PHST- 2018/04/26 00:00 [accepted] PHST- 2018/06/28 06:00 [pubmed] PHST- 2018/08/16 06:00 [medline] PHST- 2018/06/28 06:00 [entrez] AID - 000490992 [pii] AID - 10.1159/000490992 [doi] PST - ppublish SO - Cell Physiol Biochem. 2018;47(4):1696-1710. doi: 10.1159/000490992. Epub 2018 Jun 27.