PMID- 29949837 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200929 IS - 2092-7355 (Print) IS - 2092-7363 (Electronic) IS - 2092-7355 (Linking) VI - 10 IP - 4 DP - 2018 Jul TI - Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation. PG - 406-419 LID - 10.4168/aair.2018.10.4.406 [doi] AB - PURPOSE: The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. METHODS: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte-macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme-linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. RESULTS: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-gamma levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. CONCLUSIONS: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions. CI - Copyright (c) 2018 The Korean Academy of Asthma, Allergy and Clinical Immunology . The Korean Academy of Pediatric Allergy and Respiratory Disease. FAU - de Aragao-Franca, Luciana Souza AU - de Aragao-Franca LS FAU - Aragao-Franca, Luciana S AU - Aragao-Franca LS AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. AD - Centro de Biotecnologia e Terapia Celular, Hospital Sao Rafael, Salvador, Bahia, Brazil. FAU - Rocha, Viviane Costa Junqueira AU - Rocha VCJ FAU - Rocha, Viviane C J AU - Rocha VCJ AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. FAU - Cronemberger-Andrade, Andre AU - Cronemberger-Andrade A AD - Departamento de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - da Costa, Fabio Henrique Brasil AU - da Costa FHB FAU - Costa, F H B AU - Costa FHB AD - Department of Diagnostics and Biomedical Sciences at The University of Texas Health Science Center, Houston, USA. FAU - Vasconcelos, Juliana Fraga AU - Vasconcelos JF FAU - Vasconcelos, Jose Fernandes AU - Vasconcelos JF AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. AD - Centro de Biotecnologia e Terapia Celular, Hospital Sao Rafael, Salvador, Bahia, Brazil. FAU - Athanazio, Daniel Abensur AU - Athanazio DA AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. AD - Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Bahia, Brazil. FAU - Silva, Daniela Nascimento AU - Silva DN AD - Centro de Biotecnologia e Terapia Celular, Hospital Sao Rafael, Salvador, Bahia, Brazil. FAU - Santos, Emanuelle Souza AU - Santos ES FAU - Santos, E S AU - Santos ES AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. FAU - Meira, Cassio Santana AU - Meira CS AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. FAU - Araujo, Cintia Figueiredo AU - Araujo CF FAU - Araujo, C F AU - Araujo CF AD - Hospital Universitario Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, Brazil. FAU - Cerqueira, Jessica Vieira AU - Cerqueira JV AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. FAU - Cardillo, Fabiola AU - Cardillo F AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. FAU - Alcantara-Neves, Neuza Maria AU - Alcantara-Neves NM AD - Instituto de Ciencias da Saude, Universidade Federal da Bahia, Salvador, Bahia, Brazil. FAU - Soares, Milena Botelho Pereira AU - Soares MBP AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. AD - Centro de Biotecnologia e Terapia Celular, Hospital Sao Rafael, Salvador, Bahia, Brazil. milena@bahia.fiocruz.br. FAU - Pontes de Carvalho, Lain Carlos AU - Pontes de Carvalho LC FAU - Pontes-de-Carvalho, Lain C AU - Pontes-de-Carvalho LC AD - Instituto Goncalo Moniz, Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, Bahia, Brazil. LA - eng PT - Journal Article PL - Korea (South) TA - Allergy Asthma Immunol Res JT - Allergy, asthma & immunology research JID - 101518382 EIN - Allergy Asthma Immunol Res. 2018 Nov;10(6):724-725. PMID: 30306754 PMC - PMC6021587 OTO - NOTNLM OT - Dendritic cells OT - allergens, house dust mites OT - asthma OT - immunotherapy and tolerance induction COIS- There are no financial or other issues that might lead to conflict of interest. EDAT- 2018/06/28 06:00 MHDA- 2018/06/28 06:01 PMCR- 2018/07/01 CRDT- 2018/06/28 06:00 PHST- 2017/09/29 00:00 [received] PHST- 2018/02/02 00:00 [revised] PHST- 2018/02/14 00:00 [accepted] PHST- 2018/07/01 00:00 [pmc-release] PHST- 2018/06/28 06:00 [entrez] PHST- 2018/06/28 06:00 [pubmed] PHST- 2018/06/28 06:01 [medline] AID - 10.406 [pii] AID - 10.4168/aair.2018.10.4.406 [doi] PST - ppublish SO - Allergy Asthma Immunol Res. 2018 Jul;10(4):406-419. doi: 10.4168/aair.2018.10.4.406.