PMID- 29950857
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220318
IS  - 1178-6930 (Print)
IS  - 1178-6930 (Electronic)
IS  - 1178-6930 (Linking)
VI  - 11
DP  - 2018
TI  - Curcumin suppresses the progression of laryngeal squamous cell carcinoma through 
      the upregulation of miR-145 and inhibition of the PI3K/Akt/mTOR pathway.
PG  - 3521-3531
LID - 10.2147/OTT.S159236 [doi]
AB  - BACKGROUND: Curcumin is a polyphenol extracted from the rhizomes of Curcuma longa 
      with extensive biological and pharmacological effects. The present study aimed to 
      investigate the mechanisms of curcumin in laryngeal squamous cell carcinoma 
      (LSCC). METHODS: Quantitative real-time reverse transcriptase-polymerase chain 
      reaction was performed to detect the expressions of miR-145 in LSCC tissues and 
      cells. The effects of miR-145 and curcumin on cell proliferation, apoptosis, cell 
      cycle, migration and invasion were explored by MTT assay, flow cytometry 
      analysis, Transwell migration and invasion assay, respectively. The effects of 
      miR-145 combined with curcumin on the phosphoinositol 1,3 kinase (PI3K)/protein 
      kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway were detected by 
      Western blot analysis. RESULTS: miR-145 was significantly downregulated in LSCC 
      tissues and cells. Curcumin administration upregulated miR-145 expression in LSCC 
      cells in a dose-dependent manner. miR-145 overexpression and curcumin treatment 
      both markedly suppressed cell proliferation, migration and invasion and induced 
      cell cycle arrest and apoptosis in LSCC cells. Moreover, curcumin treatment 
      reversed the enhanced effects on cell viability, migration and invasion and the 
      inhibitory effects on apoptosis conferred by anti-miR-145 in LSCC cells. Curcumin 
      treatment dramatically aggravated miR-145-induced inhibition of the PI3K/Akt/mTOR 
      pathway and reversed anti-miR-145-mediated activation of the PI3K/Akt/mTOR 
      pathway in LSCC cells. CONCLUSION: Curcumin suppressed LSCC progression through 
      the upregulation of miR-145 and inhibition of the PI3K/Akt/mTOR pathway.
FAU - Zhu, Xiaofeng
AU  - Zhu X
AD  - Department of Otorhinolaryngology, Zhengzhou Central Hospital Affiliated to 
      Zhengzhou University, Zhengzhou 450000, People's Republic of China.
FAU - Zhu, Ronghua
AU  - Zhu R
AD  - Department of Otorhinolaryngology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan 250013, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20180619
PL  - New Zealand
TA  - Onco Targets Ther
JT  - OncoTargets and therapy
JID - 101514322
PMC - PMC6016259
OTO - NOTNLM
OT  - LSCC
OT  - curcumin
OT  - miR-145
OT  - the PI3K/Akt/mTOR pathway
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/06/29 06:00
MHDA- 2018/06/29 06:01
PMCR- 2018/06/19
CRDT- 2018/06/29 06:00
PHST- 2018/06/29 06:00 [entrez]
PHST- 2018/06/29 06:00 [pubmed]
PHST- 2018/06/29 06:01 [medline]
PHST- 2018/06/19 00:00 [pmc-release]
AID - ott-11-3521 [pii]
AID - 10.2147/OTT.S159236 [doi]
PST - epublish
SO  - Onco Targets Ther. 2018 Jun 19;11:3521-3531. doi: 10.2147/OTT.S159236. 
      eCollection 2018.