PMID- 29951770
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 410
IP  - 21
DP  - 2018 Aug
TI  - High-throughput and sensitive determination of urinary zearalenone and 
      metabolites by UPLC-MS/MS and its application to a human exposure study.
PG  - 5301-5312
LID - 10.1007/s00216-018-1186-4 [doi]
AB  - Biomarker-based strategies to assess human exposure to mycotoxins have gained 
      increased acceptance in recent years. In this study, an improved method based on 
      UPLC-MS/MS following 96-well muElution solid-phase extraction was developed and 
      validated for the sensitive and high-throughput determination of zearalenone 
      (ZEN) and its five metabolites alpha-zearalenol (alpha-ZEL), beta-zearalenol (beta-ZEL), 
      alpha-zearalanol (alpha-ZAL), beta-zearalanol (beta-ZAL), and zearalanone (ZAN) in human urine 
      samples, using (13)C-ZEN as an internal standard for accurate quantification. Two 
      plates of samples (n = 192) could be processed within 2 h, and baseline 
      separation of all the analytes was achieved in a total runtime of 6 min. The 
      proposed method allowed ZEN and its metabolites to be sensitively determined in a 
      high-throughput way for the first time, and with significantly improved 
      efficiency and accuracy with respect to existing methods. The limits of detection 
      (LODs) and limits of quantitation (LOQs) ranged from 0.02 to 0.06 ng mL(-1) and 
      from 0.05 to 0.2 ng mL(-1), respectively. The recoveries for the spiked samples 
      were from 87.9 to 100%, with relative standard deviations (RSDs) of less than 7%. 
      301 urine samples collected from healthy volunteers aged 0-84 years in China were 
      analyzed with and without enzyme hydrolysis to determine total and free ZEN 
      biomarkers, respectively. ZEN, ZAN, alpha-ZEL, and beta-ZEL were detected in 71.4% of 
      the samples at levels of 0.02-3.7 ng mL(-1) after enzyme hydrolysis. The 
      estimated mean probable daily intake (PDI) was much lower than the tolerable 
      daily intake (TDI). Adolescents had higher exposure than children, adults, and 
      the elderly. Graphical abstract ᅟ.
FAU - Li, Chenglong
AU  - Li C
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
FAU - Deng, Chunli
AU  - Deng C
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
FAU - Zhou, Shuang
AU  - Zhou S
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China. 
      szhoupku@gmail.com.
FAU - Zhao, Yunfeng
AU  - Zhao Y
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
FAU - Wang, Dan
AU  - Wang D
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
FAU - Wang, Xiaodan
AU  - Wang X
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
FAU - Gong, Yun Yun
AU  - Gong YY
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
AD  - School of Food Science and Nutrition, University of Leeds, Woodhouse Ln, Leeds, 
      LS2 9JT, UK.
FAU - Wu, Yongning
AU  - Wu Y
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing, 100021, China.
LA  - eng
GR  - 31501400/National Natural Science Foundation of China/
PT  - Journal Article
PT  - Validation Study
DEP - 20180627
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Estrogens, Non-Steroidal/metabolism/*urine
MH  - Female
MH  - High-Throughput Screening Assays/methods
MH  - Humans
MH  - Infant
MH  - Limit of Detection
MH  - Male
MH  - Middle Aged
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Young Adult
MH  - Zearalenone/metabolism/*urine
OTO - NOTNLM
OT  - 96-well muElution SPE
OT  - Biomonitoring
OT  - Metabolites
OT  - UPLC-MS/MS
OT  - Urine
OT  - Zearalenone
EDAT- 2018/06/29 06:00
MHDA- 2018/08/15 06:00
CRDT- 2018/06/29 06:00
PHST- 2017/12/27 00:00 [received]
PHST- 2018/06/05 00:00 [accepted]
PHST- 2018/05/28 00:00 [revised]
PHST- 2018/06/29 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2018/06/29 06:00 [entrez]
AID - 10.1007/s00216-018-1186-4 [pii]
AID - 10.1007/s00216-018-1186-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2018 Aug;410(21):5301-5312. doi: 10.1007/s00216-018-1186-4. 
      Epub 2018 Jun 27.