PMID- 29951932
OWN - NLM
STAT- MEDLINE
DCOM- 20181025
LR  - 20181114
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 38
IP  - 6
DP  - 2018 Aug
TI  - Minocycline Promotes BDNF Expression of N2a Cells via Inhibition of 
      miR-155-Mediated Repression After Oxygen-Glucose Deprivation and Reoxygenation.
PG  - 1305-1313
LID - 10.1007/s10571-018-0599-0 [doi]
AB  - Minocycline, an anti-infective agent of a tetracycline derivative, is reported to 
      improve behavioral functional recovery after cerebral ischemia via enhancing the 
      levels of brain-derived neurotrophic factor (BDNF). However, the precise 
      mechanisms that minocycline targets to enhance the expression of BDNF are not 
      fully defined. In the present study, we observed the neuroprotective effect and 
      its potential mechanisms of minocycline using oxygen-glucose 
      deprivation/reoxygenation (OGD/R)-treated N2a cells. We found that 50 microM 
      minocycline protected against neuronal apoptosis induced by OGD/R injury, with 
      increased expression ratio of Bcl-2/Bax and reduced expression of caspase-3. 
      Interestingly, minocycline resulted in the up-regulation of only BDNF protein, 
      not BDNF mRNA in N2a cells treated with OGD/R. Furthermore, we found that 
      minocycline inhibited OGD/R-induced up-regulation of miR-155 targeted BDNF 
      transcripts. Moreover, miR-155 mimic could partially abolish the neuroprotective 
      effects of minocycline via inhibiting the levels of BDNF protein. These findings 
      suggest that minocycline is neuroprotective against ischemic brain injury through 
      their modulation of miR-155-mediated BDNF repression.
FAU - Lu, Yunnan
AU  - Lu Y
AD  - Department of Neurology, The Affiliated Wuxi No.2 People's Hospital of Nanjing 
      Medical University, Wuxi, 214000, Jiangsu, China.
FAU - Huang, Zhichao
AU  - Huang Z
AD  - Department of Neurology, The Second Affiliated Hospital of Soochow University, 
      1055 Sanxiang Rd, Gusu District, Suzhou, 215004, Jiangsu, China.
FAU - Hua, Ye
AU  - Hua Y
AD  - Department of Neurology, The Affiliated Wuxi No.2 People's Hospital of Nanjing 
      Medical University, Wuxi, 214000, Jiangsu, China.
FAU - Xiao, Guodong
AU  - Xiao G
AD  - Department of Neurology, The Second Affiliated Hospital of Soochow University, 
      1055 Sanxiang Rd, Gusu District, Suzhou, 215004, Jiangsu, China. 
      Parsley_shaw@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20180627
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (Neuroprotective Agents)
RN  - FYY3R43WGO (Minocycline)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain/drug effects/metabolism
MH  - Brain Ischemia/*genetics/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Glucose/*metabolism
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Minocycline/*pharmacology
MH  - Neurons/metabolism
MH  - Neuroprotective Agents/metabolism
MH  - Oxygen/*metabolism
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - BDNF
OT  - Ischemia stroke
OT  - MiR-155
OT  - Minocycline
OT  - Oxygen-glucose deprivation and reoxygenation
EDAT- 2018/06/29 06:00
MHDA- 2018/10/26 06:00
CRDT- 2018/06/29 06:00
PHST- 2018/04/26 00:00 [received]
PHST- 2018/06/21 00:00 [accepted]
PHST- 2018/06/29 06:00 [pubmed]
PHST- 2018/10/26 06:00 [medline]
PHST- 2018/06/29 06:00 [entrez]
AID - 10.1007/s10571-018-0599-0 [pii]
AID - 10.1007/s10571-018-0599-0 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2018 Aug;38(6):1305-1313. doi: 10.1007/s10571-018-0599-0. 
      Epub 2018 Jun 27.