PMID- 29953168
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230207
IS  - 1934-8258 (Electronic)
IS  - 1934-8258 (Linking)
VI  - 98
IP  - 1
DP  - 2018 Jul
TI  - Preparation, Culture, and Analysis of Amniotic Fluid Samples.
PG  - e62
LID - 10.1002/cphg.62 [doi]
AB  - Amniotic fluid obtained via amniocentesis provides a source of fetal material 
      used in prenatal diagnosis. The fluid may be used directly for biochemical 
      analyses, fluorescence in situ hybridization (FISH), and isolation of DNA for 
      molecular studies, including chromosomal microarray analysis (CMA). The fluid is 
      typically cultured as a source of metaphase cells for chromosome analysis and to 
      provide additional material for biochemical and DNA-based testing. This unit 
      describes an in situ method for the preparation, culture, and harvest of amniotic 
      fluid samples for metaphase chromosome analysis. Cells are grown, harvested for 
      metaphase spreads, and analyzed on glass coverslips. The unit also describes 
      methods to obtain cells for additional studies (such as molecular genetic 
      analyses) by growing cells in flasks either following passaging of cells from a 
      glass coverslip culture or by directly establishing a flask culture from the 
      amniotic fluid specimen. When cells are grown in flasks, they must be removed 
      from the flask with trypsin before they can be used in studies. Lastly, this unit 
      describes a method for isolating DNA for CMA from uncultured amniotic fluid and 
      cultured cells. (c) 2018 by John Wiley & Sons, Inc.
CI  - (c) 2018 John Wiley & Sons, Inc.
FAU - Miron, Patricia Minehart
AU  - Miron PM
AD  - UMass Memorial Medical Center, Worcester, Massachusetts.
LA  - eng
PT  - Journal Article
DEP - 20180628
PL  - United States
TA  - Curr Protoc Hum Genet
JT  - Current protocols in human genetics
JID - 101287858
OTO - NOTNLM
OT  - amniotic fluid
OT  - clinical
OT  - cytogenetics
OT  - metaphase chromosomes
OT  - prenatal diagnosis
EDAT- 2018/06/29 06:00
MHDA- 2018/06/29 06:01
CRDT- 2018/06/29 06:00
PHST- 2018/06/29 06:00 [pubmed]
PHST- 2018/06/29 06:01 [medline]
PHST- 2018/06/29 06:00 [entrez]
AID - 10.1002/cphg.62 [doi]
PST - ppublish
SO  - Curr Protoc Hum Genet. 2018 Jul;98(1):e62. doi: 10.1002/cphg.62. Epub 2018 Jun 
      28.