PMID- 29953671 OWN - NLM STAT- MEDLINE DCOM- 20190515 LR - 20190515 IS - 1099-1166 (Electronic) IS - 0885-6230 (Linking) VI - 33 IP - 10 DP - 2018 Oct TI - The influence of aging on the methylation status of brain-derived neurotrophic factor gene in blood. PG - 1312-1318 LID - 10.1002/gps.4927 [doi] AB - OBJECTIVE: Brain-derived neurotrophic factor (BDNF) is involved in the pathophysiology of psychiatric disorders in adults and elderly individuals, and as a result, the DNA methylation (DNAm) of the BDNF gene in peripheral tissues including blood has been extensively examined to develop a useful biomarker for psychiatric disorders. However, studies to date have not previously investigated the effect of age on DNAm of the BDNF gene in blood. In this context, we measured DNAm of 39 CpG units in the CpG island at the promoter of exon I of the BDNF gene. METHODS: We analyzed genomic DNA from peripheral blood of 105 health Japanese women 20 to 80 years of age to identify aging-associated change in DNAm of the BDNF gene. In addition, we examined the relationship between total MMSE scores, numbers of stressful life events, and serum BDNF levels on DNAm of the BDNF gene. The DNAm rate at each CpG unit was measured using a MassArray((R)) system (Agena Bioscience), and serum BDNF levels were measured by ELISA. RESULTS: There was a significant correlation between DNAm and age in 13 CpGs. However, there was no significant correlation between DNAm and total MMSE scores, numbers of life events, or serum BDNF levels. CONCLUSION: Despite the small number of subjects and the inclusion of only female subjects, our results suggest that DNAm of 13 CpGs of the BDNF gene may be an appropriate biomarker for aging and useful for predicting increased susceptibility to age-related psychiatric disorders. CI - (c) 2018 John Wiley & Sons, Ltd. FAU - Ihara, Kazushige AU - Ihara K AD - Hirosaki University Graduate School of Medicine, Department of Social Medicine, Aomori, Japan. FAU - Fuchikami, Manabu AU - Fuchikami M FAU - Hashizume, Masahiro AU - Hashizume M AD - Toho University Faculty of Medicine, Department of Psychosomatic Medicine, Tokyo, Japan. FAU - Okada, Satoshi AU - Okada S AD - Hiroshima University, Department of Psychiatry and Neurosciences, Division of Frontier Graduate School of Biomedical Sciences, Hiroshima, Japan. FAU - Kawai, Hisashi AU - Kawai H AD - Tokyo Metropolitan Institute of Gerontology, Human Care Research Team, Tokyo, Japan. FAU - Obuchi, Shuichi AU - Obuchi S AD - Tokyo Metropolitan Institute of Gerontology, Human Care Research Team, Tokyo, Japan. FAU - Hirano, Hirohiko AU - Hirano H AD - Tokyo Metropolitan Geriatric Hospital, Department of Dentistry, Tokyo, Japan. FAU - Fujiwara, Yoshinori AU - Fujiwara Y AD - Tokyo Metropolitan Institute of Gerontology, Research Team for Social Participation and Community Health, Tokyo, Japan. FAU - Hachisu, Mitsugu AU - Hachisu M AD - Showa University, Department of Pharmaceutical therapeutics, Division of Clinical Pharmacy, Pharmacy School, Tokyo, Japan. FAU - Hongyong, Kim AU - Hongyong K AD - Tokyo Metropolitan Institute of Gerontology, Research Team for Promoting Independence of the Elderly, Tokyo, Japan. FAU - Morinobu, Shigeru AU - Morinobu S AUID- ORCID: 0000-0002-7496-7519 AD - Kochi University, Department of Neuropsychiatry, Kochi Medical School, Nankoku, Japan. AD - Kibi International University, Department of Occupational Therapy, School of Health Science and Social Welfare, Takahashi, Japan. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180628 PL - England TA - Int J Geriatr Psychiatry JT - International journal of geriatric psychiatry JID - 8710629 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Dinucleoside Phosphates) RN - 0 (Genetic Markers) RN - 2382-65-2 (cytidylyl-3'-5'-guanosine) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Aging/genetics/*physiology MH - Brain-Derived Neurotrophic Factor/blood/*physiology MH - CpG Islands MH - DNA Methylation/*physiology MH - Dinucleoside Phosphates MH - Female MH - Genetic Markers/physiology MH - Humans MH - Male MH - Mental Disorders/genetics/*metabolism MH - Middle Aged MH - Promoter Regions, Genetic MH - Young Adult OTO - NOTNLM OT - DNA methylation OT - aging OT - brain-derived neurotrophic factor (BDNF) EDAT- 2018/06/29 06:00 MHDA- 2019/05/16 06:00 CRDT- 2018/06/29 06:00 PHST- 2017/12/29 00:00 [received] PHST- 2018/05/08 00:00 [accepted] PHST- 2018/06/29 06:00 [pubmed] PHST- 2019/05/16 06:00 [medline] PHST- 2018/06/29 06:00 [entrez] AID - 10.1002/gps.4927 [doi] PST - ppublish SO - Int J Geriatr Psychiatry. 2018 Oct;33(10):1312-1318. doi: 10.1002/gps.4927. Epub 2018 Jun 28.