PMID- 29955707
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240421
IS  - 2475-2991 (Electronic)
IS  - 2475-2991 (Linking)
VI  - 1
IP  - 6
DP  - 2017 Jun
TI  - Ingesting a Combined Carbohydrate and Essential Amino Acid Supplement Compared to 
      a Non-Nutritive Placebo Blunts Mitochondrial Biogenesis-Related Gene Expression 
      after Aerobic Exercise.
PG  - e000893
LID - 10.3945/cdn.117.000893 [doi]
LID - e000893
AB  - Background: Whether load carriage (LC), an endurance exercise mode composed of 
      the aerobic component of traditional endurance exercise [e.g., cycle ergometry 
      (CE)] and contractile forces characteristic of resistive-type exercise, modulates 
      acute mitochondrial adaptive responses to endurance exercise and supplemental 
      nutrition [carbohydrate + essential amino acids (CHO+EAA)] is not known. 
      Objective: The aim of this study was to examine the effects of LC and CE, with or 
      without CHO+EAA supplementation, on acute markers of mitochondrial biogenesis. 
      Methods: Twenty-five adults performed 90 min of metabolically matched LC 
      (treadmill walking, wearing a vest equal to 30% of body mass) or CE exercise 
      during which CHO+EAA (46 g carbohydrate and 10 g essential amino acids) or 
      non-nutritive control (CON) drinks were consumed. Muscle biopsy samples were 
      collected at rest (pre-exercise), post-exercise, and after 3 h of recovery to 
      assess citrate synthase activity and the expression of mRNA (reverse 
      transcriptase-quantitative polymerase chain reaction) and protein (Western blot). 
      Results: Citrate synthase and phosphorylated p38 mitogen-activated protein kinase 
      (p38 MAPK)(Thr180/Tyr182) were elevated postexercise compared with pre-exercise 
      (time main effect, P < 0.05). Peroxisome proliferator-activated gamma-receptor 
      coactivator 1alpha (PGC-1alpha) expression was highest after recovery for CE compared 
      with LC (exercise-by-time effect, P < 0.05). Sirtuin 1 (SIRT1) expression 
      postexercise was higher for CON than for CHO+EAA treatments (drink-by-time, P < 
      0.05). Tumor suppressor p53 (p53), mitochondrial transcription factor A (TFAM), 
      and cytochrome c oxidase subunit IV (COXIV) expression was greater for CON than 
      for CHO+EAA treatments (drink main effect, P < 0.05). PGC-1alpha and p53 expressions 
      were positively associated (P < 0.05) with TFAM (r = 0.629 and 0.736, 
      respectively) and COXIV (r = 0.465 and 0.461, respectively) expressions. 
      Conclusions: Acute mitochondrial adaptive responses to endurance exercise appear 
      to be largely driven by exogenous nutrition availability. Although CE upregulated 
      PGC-1alpha expression to a greater extent than LC, downstream signaling was the same 
      between modes, suggesting that LC, in large part, elicits the same acute 
      mitochondrial response as traditional, non-weight-bearing endurance exercise. 
      This trial was registered at clinicaltrials.gov as NCT01714479.
FAU - Margolis, Lee M
AU  - Margolis LM
AD  - Military Nutrition Division, US Army Research Institute of Environmental 
      Medicine, Natick, MA.
AD  - Oak Ridge Institute for Science and Education, Oak Ridge, TN.
FAU - Murphy, Nancy E
AU  - Murphy NE
AD  - Military Nutrition Division, US Army Research Institute of Environmental 
      Medicine, Natick, MA.
FAU - Carrigan, Christopher T
AU  - Carrigan CT
AD  - Military Nutrition Division, US Army Research Institute of Environmental 
      Medicine, Natick, MA.
AD  - Oak Ridge Institute for Science and Education, Oak Ridge, TN.
FAU - McClung, Holly L
AU  - McClung HL
AD  - Military Nutrition Division, US Army Research Institute of Environmental 
      Medicine, Natick, MA.
FAU - Pasiakos, Stefan M
AU  - Pasiakos SM
AD  - Military Nutrition Division, US Army Research Institute of Environmental 
      Medicine, Natick, MA.
LA  - eng
SI  - ClinicalTrials.gov/NCT01714479
PT  - Journal Article
DEP - 20170523
PL  - United States
TA  - Curr Dev Nutr
JT  - Current developments in nutrition
JID - 101717957
PMC - PMC5998348
OTO - NOTNLM
OT  - COXIV
OT  - PGC-1alpha
OT  - SIRT1
OT  - TFAM
OT  - concurrent exercise
OT  - p53
EDAT- 2018/06/30 06:00
MHDA- 2018/06/30 06:01
PMCR- 2017/05/23
CRDT- 2018/06/30 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/05/06 00:00 [revised]
PHST- 2017/05/22 00:00 [accepted]
PHST- 2018/06/30 06:00 [entrez]
PHST- 2018/06/30 06:00 [pubmed]
PHST- 2018/06/30 06:01 [medline]
PHST- 2017/05/23 00:00 [pmc-release]
AID - S2475-2991(22)14466-5 [pii]
AID - 000893 [pii]
AID - 10.3945/cdn.117.000893 [doi]
PST - epublish
SO  - Curr Dev Nutr. 2017 May 23;1(6):e000893. doi: 10.3945/cdn.117.000893. eCollection 
      2017 Jun.