PMID- 29956151
OWN - NLM
STAT- MEDLINE
DCOM- 20190228
LR  - 20230925
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1799
DP  - 2018
TI  - The Identification of Allergen-Derived T Cell Epitopes.
PG  - 153-163
LID - 10.1007/978-1-4939-7896-0_13 [doi]
AB  - Type I allergy is a disease primarily mediated by immunoglobulin E (IgE) and T 
      helper type 2 (Th2) cells. The role of Th2 and other T cell subsets in the 
      pathology of allergic disease as well as induction of tolerance has become an 
      area of intense research over the last decades. Studying allergen-specific T 
      cells to gain a better understanding of their contribution to allergic pathology 
      and how they are modulated by allergen-specific immunotherapy requires knowledge 
      of the allergens targeted by these cells. Identification of T cell epitopes in 
      allergy can be achieved by a variety of methods. In this chapter, we will focus 
      on a technique named FluoroSpot, which relies on the detection of cytokines 
      secreted by T cells in response to stimulation with an antigen (allergen), such 
      as timothy grass (TG) extract or an allergen-derived peptide, for which the cell 
      is specific. We will describe how to overcome the challenge of detecting rare, 
      TG-specific, T cells that occur at low frequency in the blood by using an in 
      vitro expansion culture and subsequent mapping of the precise T cell epitope 
      using FluoroSpot.
FAU - Schulten, Veronique
AU  - Schulten V
AD  - Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La 
      Jolla, CA, USA. veronique@lji.org.
FAU - Sette, Alessandro
AU  - Sette A
AD  - Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La 
      Jolla, CA, USA.
LA  - eng
GR  - U19 AI100275/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, T-Lymphocyte)
SB  - IM
MH  - Allergens/chemistry/*immunology
MH  - Amino Acid Sequence
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Data Analysis
MH  - *Epitope Mapping/methods
MH  - Epitopes, T-Lymphocyte/chemistry/*immunology
MH  - Humans
MH  - Lymphocyte Activation
MH  - T-Lymphocyte Subsets/immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
OTO - NOTNLM
OT  - Allergy
OT  - Cytokine
OT  - FluoroSpot
OT  - T cell epitope
OT  - T helper 2 cells
OT  - Timothy grass
EDAT- 2018/06/30 06:00
MHDA- 2019/03/01 06:00
CRDT- 2018/06/30 06:00
PHST- 2018/06/30 06:00 [entrez]
PHST- 2018/06/30 06:00 [pubmed]
PHST- 2019/03/01 06:00 [medline]
AID - 10.1007/978-1-4939-7896-0_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1799:153-163. doi: 10.1007/978-1-4939-7896-0_13.