PMID- 29956585
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20231103
IS  - 1744-8069 (Electronic)
IS  - 1744-8069 (Linking)
VI  - 14
DP  - 2018 Jan-Dec
TI  - Nuclear factor kappa B regulated monocyte chemoattractant protein-1/chemokine CC 
      motif receptor-2 expressing in spinal cord contributes to the maintenance of 
      cancer-induced bone pain in rats.
PG  - 1744806918788681
LID - 10.1177/1744806918788681 [doi]
LID - 1744806918788681
AB  - BACKGROUND: Chemokine, monocyte chemoattractant protein-1 (MCP-1), is a potential 
      factor to cause cancer-induced bone pain (CIBP). NF-kappaB signaling is very 
      important in mediating the expression of chemokines and may have a role in CIBP. 
      However, the mechanism is still unclear. This study investigates the role of 
      NF-kappaB in CIBP by regulating MCP-1/chemokine CC motif receptor-2 (CCR2) signaling 
      pathway. METHODS: A rat CIBP model was established by injecting Walker-256 cells 
      into the tibia medullary cavity. Nine days later, animals were intrathecally 
      administrated with MCP-1 neutralizing antibody, CCR2 antagonist (RS504393), or 
      NF-small ka, CyrillicB inhibitor (BAY11-7081). Mechanical paw withdrawal threshold was used to 
      assess pain behavior and sciatic functional index, and radiographic images were 
      adopted to evaluate the damage of nerve and bone. The spinal cords were harvested 
      for Western blot and quantitative reverse transcription polymerase chain 
      reaction. The distribution of MCP-1, CCR2, and NF-small ka, CyrillicB was detected by double 
      immunofluorescent staining. RESULTS: CIBP caused remarkable bone destruction, 
      injury of sciatic and femoral nerve, and persistent (>15 days) mechanical 
      allodynia in rats. Tumor cell inoculation upregulate MCP-1 and NF-small ka, CyrillicB in activated 
      neurons as well as CCR2 in neurons and microglia of the spinal cord. MCP-1 
      antibody, RS504393, and BAY11-7081 partially reversed CIBP-induced mechanical 
      allodynia, and CIBP regulated the expression levels of pro-inflammatory 
      cytokines, tumor necrosis factor-alpha and interferon-gamma, and anti-inflammatory 
      cytokine, interleukin 4, and BAY11-7081 lowered CIBP-induced MCP-1 and CCR2 
      expressions in a dose-dependent manner. CONCLUSION: In conclusion, NF-small ka, CyrillicB 
      signaling pathway regulates the expressions of MCP-1/CCR2-induced inflammatory 
      factors in the spinal cord of CIBP rats.
FAU - Wang, Yungong
AU  - Wang Y
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Ni, Huadong
AU  - Ni H
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
AD  - 2 The Second Clinical Medical College, Zhejiang Chinese Medicine University, 
      Hangzhou, China.
FAU - Li, Hongbo
AU  - Li H
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Deng, Houshen
AU  - Deng H
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Xu, Long S
AU  - Xu LS
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Xu, Shijie
AU  - Xu S
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Zhen, Ying
AU  - Zhen Y
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Shen, Hui
AU  - Shen H
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Pan, Huan
AU  - Pan H
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
FAU - Yao, Ming
AU  - Yao M
AUID- ORCID: 0000-0002-4226-8473
AD  - 1 Department of Anesthesiology and Pain Medicine, First Affiliated Hospital of 
      Jiaxing University, Jiaxing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180629
PL  - United States
TA  - Mol Pain
JT  - Molecular pain
JID - 101242662
RN  - 0 (3-(4-methylphenylsulfonyl)-2-propenenitrile)
RN  - 0 (Antibodies)
RN  - 0 (Benzoxazines)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Nitriles)
RN  - 0 (RS 504393)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Spiro Compounds)
RN  - 0 (Sulfones)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - Antibodies/therapeutic use
MH  - Benzoxazines/therapeutic use
MH  - Bone Neoplasms/complications/diagnostic imaging
MH  - Cancer Pain/diagnostic imaging/etiology/*pathology
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/immunology/*metabolism
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - NF-kappa B/*metabolism
MH  - Nitriles/therapeutic use
MH  - Pain Threshold/drug effects
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, CCR2/*metabolism
MH  - Spinal Cord/*metabolism
MH  - Spiro Compounds/therapeutic use
MH  - Sulfones/therapeutic use
MH  - Time Factors
MH  - Up-Regulation/drug effects
PMC - PMC6055241
OTO - NOTNLM
OT  - Cancer-induced bone pain
OT  - MCP-1/CCR2 axis
OT  - NF-kappaB signaling pathway
OT  - astrocytes
OT  - chemokines
OT  - neurons
EDAT- 2018/06/30 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/06/29
CRDT- 2018/06/30 06:00
PHST- 2018/06/30 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/06/30 06:00 [entrez]
PHST- 2018/06/29 00:00 [pmc-release]
AID - 10.1177_1744806918788681 [pii]
AID - 10.1177/1744806918788681 [doi]
PST - ppublish
SO  - Mol Pain. 2018 Jan-Dec;14:1744806918788681. doi: 10.1177/1744806918788681. Epub 
      2018 Jun 29.