PMID- 29959084
OWN - NLM
STAT- MEDLINE
DCOM- 20191021
LR  - 20210218
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Linking)
VI  - 185
DP  - 2018 Aug 15
TI  - Serum responsive proteome reveals correlation between oxidative phosphorylation 
      and morphogenesis in Candida albicans ATCC10231.
PG  - 25-38
LID - S1874-3919(18)30262-8 [pii]
LID - 10.1016/j.jprot.2018.06.018 [doi]
AB  - To understand the impact of fetal bovine serum (FBS) on metabolism and cellular 
      architecture in addition to morphogenesis, we have identified FBS responsive 
      proteome of Candida albicans. FBS induced 34% hyphae and 60% pseudohyphae in C. 
      albicans at 30  degrees C while 98% hyphae at 37  degrees C. LC-MS/MS analysis revealed that 285 
      proteins modulated significantly in response to FBS at 30  degrees C and 37  degrees C. Out of 
      which 152 were upregulated and 62 were downregulated at 30  degrees C while 18 were up 
      and 53 were downregulated at 37  degrees C. Functional annotation suggests that FBS may 
      inhibit glycolysis and fermentative pathway and enhance oxidative phosphorylation 
      (OxPhos), TCA cycle, amino acid and fatty acid metabolism indicating a use of 
      alternative energy source by C. albicans. OxPhos inhibition assay using sodium 
      azide corroborated the correlation between inhibition of glycolysis and enhanced 
      OxPhos with pseudohyphae formation. C. albicans induced hyphae in response to FBS 
      irrespective of down regulation of Ras1,Asr1/Asr2, indicates the possible 
      involvement of MAPK and cAMP-PKA independent pathway. The Cell wall of cells 
      grown in presence of FBS at 30  degrees C was rich in mannan, Beta 1,3-glucan and chitin 
      while membranes were rich in ergosterol compared to those grown at 37  degrees C. 
      SIGNIFICANCE OF THE STUDY: This is the first study suggesting a correlation 
      between OxPhos and morphogenesis especially pseudohyphae formation in C. 
      albicans. Our data also indicate that fetal bovine serum (FBS) induced 
      morphogenesis is multifactorial and may involve MAPK and cAMP-PKA independent 
      pathway. In addition to morphogenesis, our study provides an insight in to the 
      modulation of metabolism and cellular architecture of C. albicans in response to 
      FBS.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Ahmed, Radfan
AU  - Ahmed R
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India.
FAU - Kodgire, Santosh
AU  - Kodgire S
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India.
FAU - Santhakumari, B
AU  - Santhakumari B
AD  - CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, MS, India. 
      Electronic address: b.santhakumari@ncl.res.in.
FAU - Patil, Rajendra
AU  - Patil R
AD  - Department of Biotechnology, Savitribai Phule Pune University, Ganeshkhind, Pune 
      411007, MS, India. Electronic address: rpatil@unipune.ac.in.
FAU - Kulkarni, Mahesh
AU  - Kulkarni M
AD  - CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, MS, India. 
      Electronic address: mj.kulkarni@ncl.res.in.
FAU - Zore, Gajanan
AU  - Zore G
AD  - School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 
      431606, MS, India. Electronic address: gbzsrtmun@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180626
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
RN  - 0 (Fungal Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Candida albicans/*growth & development/*metabolism
MH  - Cattle
MH  - Chromatography, Liquid
MH  - Fungal Proteins/analysis/metabolism
MH  - Hyphae/growth & development/metabolism
MH  - Morphogenesis/*physiology
MH  - *Oxidative Phosphorylation
MH  - Proteome/*metabolism
MH  - Serum/*physiology
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Candida albicans
OT  - Fetal bovin serum
OT  - Liquid chromatography-mass spectrometry analysis
OT  - Morphogenesis
OT  - Oxidative phosphorylation
OT  - Pathogenicity
EDAT- 2018/07/01 06:00
MHDA- 2019/10/23 06:00
CRDT- 2018/07/01 06:00
PHST- 2018/04/10 00:00 [received]
PHST- 2018/06/05 00:00 [revised]
PHST- 2018/06/18 00:00 [accepted]
PHST- 2018/07/01 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2018/07/01 06:00 [entrez]
AID - S1874-3919(18)30262-8 [pii]
AID - 10.1016/j.jprot.2018.06.018 [doi]
PST - ppublish
SO  - J Proteomics. 2018 Aug 15;185:25-38. doi: 10.1016/j.jprot.2018.06.018. Epub 2018 
      Jun 26.