PMID- 29959625
OWN - NLM
STAT- MEDLINE
DCOM- 20190109
LR  - 20190109
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 41
IP  - 5
DP  - 2018 Oct
TI  - Spilanthol Inhibits COX-2 and ICAM-1 Expression via Suppression of NF-kappaB and MAPK 
      Signaling in Interleukin-1beta-Stimulated Human Lung Epithelial Cells.
PG  - 1934-1944
LID - 10.1007/s10753-018-0837-0 [doi]
AB  - Spilanthol a phytochemical derived from the Spilanthes acmella plant has 
      antimicrobial, antioxidant, and anti-inflammatory properties. This study 
      evaluated its effects on the expression of intercellular adhesion molecule 1 
      (ICAM-1) and inflammation-related mediators in IL-1beta-stimulated human lung 
      epithelial A549 cells. Human lung epithelial A549 cells were pretreated with 
      various concentrations of spilanthol (3-100 muM) followed by treatment with IL-1beta 
      to induce inflammation. The protein levels of pro-inflammatory cytokines, 
      chemokines, and prostaglandin E2 (PGE2) were measured using ELISA. 
      Cyclooxygenase-2 (COX-2), heme oxygenase (HO-1), nuclear transcription factor 
      kappa-B (NF-kappaB), and mitogen-activated protein kinase (MAPK) were measured by 
      immunoblotting. The mRNA expression levels of ICAM-1 and MUC5AC were determined 
      by real-time polymerase chain reaction. Spilanthol decreased the expression of 
      PGE(2), COX-2, TNF-alpha, and MCP-1. It also decreased ICAM-1 expression and 
      suppressed monocyte adhesion to IL-1beta-stimulated A549 cells. Spilanthol also 
      significantly inhibited the phosphorylation of MAPK and I-kappaB. These results 
      suggest that spilanthol exerts anti-inflammatory effects by inhibiting the 
      expression of the pro-inflammatory cytokines, COX-2, and ICAM-1 by inhibiting the 
      NF-kappaB and MAPK signaling pathways. Graphical Abstract ᅟ.
FAU - Huang, Wen-Chung
AU  - Huang WC
AD  - Graduate Institute of Health Industry Technology, Research Center for Industry of 
      Human Ecology, Research Center for Chinese Herbal Medicine, College of Human 
      Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., 
      Guishan Dist., Taoyuan, 33303, Taiwan.
AD  - Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang 
      Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan, 33303, Taiwan.
FAU - Wu, Ling-Yu
AU  - Wu LY
AD  - Department of Nutrition and Health Sciences, Research Center for Food and 
      Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of 
      Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 
      1st Rd., Guishan Dist., Taoyuan, 33303, Taiwan.
FAU - Hu, Sindy
AU  - Hu S
AD  - Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial 
      Hospital, Guishan Dist., Taoyuan, 33303, Taiwan.
AD  - Department of Cosmetic Science, Research Center for Food and Cosmetic Safety, and 
      Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung 
      University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., 
      Taoyuan, 33303, Taiwan.
FAU - Wu, Shu-Ju
AU  - Wu SJ
AD  - Graduate Institute of Health Industry Technology, Research Center for Industry of 
      Human Ecology, Research Center for Chinese Herbal Medicine, College of Human 
      Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., 
      Guishan Dist., Taoyuan, 33303, Taiwan. sjwu@gw.cgust.edu.tw.
AD  - Department of Nutrition and Health Sciences, Research Center for Food and 
      Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of 
      Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 
      1st Rd., Guishan Dist., Taoyuan, 33303, Taiwan. sjwu@gw.cgust.edu.tw.
AD  - Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial 
      Hospital, Guishan Dist., Taoyuan, 33303, Taiwan. sjwu@gw.cgust.edu.tw.
LA  - eng
GR  - MOST 105-2320-B-255-004/Ministry of Science and Technology in Taiwan/
GR  - EZRPF3FG0071/Chang Gung University of Science and Technology/
GR  - CMRPF1G0201/Chang Gung Memorial Hospital, Linkou/
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1beta)
RN  - 0 (N-isobutyl-2E-decenamide)
RN  - 0 (NF-kappa B)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - A549 Cells
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cyclooxygenase 2/drug effects/*metabolism
MH  - Epithelial Cells/*metabolism
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Intercellular Adhesion Molecule-1/drug effects/*metabolism
MH  - Interleukin-1beta/pharmacology
MH  - Mitogen-Activated Protein Kinases/*antagonists & inhibitors
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Phosphorylation/drug effects
MH  - Polyunsaturated Alkamides/*pharmacology
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - ICAM-1
OT  - MAPK
OT  - NF-kappaB
OT  - chemokines
OT  - spilanthol
EDAT- 2018/07/01 06:00
MHDA- 2019/01/10 06:00
CRDT- 2018/07/01 06:00
PHST- 2018/07/01 06:00 [pubmed]
PHST- 2019/01/10 06:00 [medline]
PHST- 2018/07/01 06:00 [entrez]
AID - 10.1007/s10753-018-0837-0 [pii]
AID - 10.1007/s10753-018-0837-0 [doi]
PST - ppublish
SO  - Inflammation. 2018 Oct;41(5):1934-1944. doi: 10.1007/s10753-018-0837-0.