PMID- 29961347
OWN - NLM
STAT- MEDLINE
DCOM- 20190111
LR  - 20190111
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 32
IP  - 5
DP  - 2018 Sep
TI  - TREM-1 Neutrophil Activation Pathway Is Suppressed in Eosinophilic Nasal Polyps.
PG  - 359-368
LID - 10.1177/1945892418782233 [doi]
AB  - Background Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by 
      type 2 helper T-cell-skewed immune profiles, but the state of the neutrophil 
      activation signaling pathway in CRSwNP is unknown. The purpose of this study was 
      to examine neutrophil activation pathways in the pathogenesis of CRSwNP. Methods 
      Institutional review board approved the study in which tissue proteomes were 
      compared between control (inferior turbinate) and CRSwNP (nasal polyps) 
      (n = 10/group) using an aptamer-based proteomic array and confirmed by whole 
      transcriptomic analysis. Protein expression was analyzed using Student's t test 
      and Benjamini-Hochberg procedures followed by the application of Ingenuity 
      Pathway, MetaCore, and Genemania bioinformatics analyses. Results All the 
      patients with CRSwNP (n = 10) had eosinophilic nasal polyps. Compared with 
      controls, proteins associated with the triggering receptor expressed on myeloid 
      cells 1 (TREM-1) neutrophil activation signaling pathway such as Calcineurin B, 
      zeta chain-associated protein kinase of 70 kD (ZAP70), 14-3-3 protein theta, 
      14-3-3 protein zeta/delta, protein kinase C delta type (PKC-D), Interleukin 
      (IL)-17B, IL-17B receptor, IL-23, and IL-1B were significantly decreased in 
      CRSwNP (fold change [FC] = -1.60, P = .003; FC = -1.85, P = .040; FC = -1.26, 
      P < .001; FC = -1.05, P = .008; and FC = -1.31, P = .004; FC = -1.22, P < .001; 
      FC = -1.09, P = .022; FC = -1.25, P < .001; and FC = -1.31, P = .014; 
      respectively). In contrast, tissue eosinophil count ( P < .001) and 
      eosinophil-associated proteins such as C-C motif chemokine 17, periostin, and 
      galectin 10 were all significantly increased in CRSwNP (FC = 1.56, P = .009; 
      FC = 3.95, P < .001; and FC = 2.44, P < .001; respectively). Furthermore, the FC 
      of the studied proteins' expression significantly and positively correlated with 
      FC of their mRNA expression ( P = .001, r = .75). Conclusions TREM-1-associated 
      neutrophilic signaling pathway proteins are significantly suppressed in 
      eosinophilic CRSwNP.
FAU - Wu, Dawei
AU  - Wu D
AD  - 1 Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard 
      Medical School, Boston, Massachusetts.
AD  - 2 Department of Otolaryngology, Beijing Anzhen Hospital, Capital Medical 
      University, Beijing, PR China.
FAU - Mueller, Sarina K
AU  - Mueller SK
AD  - 1 Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard 
      Medical School, Boston, Massachusetts.
AD  - 3 Department of Otolaryngology, University of Erlangen-Nuremberg, Erlangen, 
      Germany.
FAU - Nocera, Angela L
AU  - Nocera AL
AD  - 1 Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard 
      Medical School, Boston, Massachusetts.
FAU - Finn, Kristen
AU  - Finn K
AD  - 1 Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard 
      Medical School, Boston, Massachusetts.
FAU - Libermann, Towia A
AU  - Libermann TA
AD  - 4 Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess 
      Medical Center, Harvard Medical School, Boston, Massachusetts.
FAU - Bleier, Benjamin S
AU  - Bleier BS
AD  - 1 Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard 
      Medical School, Boston, Massachusetts.
LA  - eng
PT  - Journal Article
DEP - 20180702
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (Proteome)
RN  - 0 (TREM1 protein, human)
RN  - 0 (Triggering Receptor Expressed on Myeloid Cells-1)
SB  - IM
MH  - Adult
MH  - Cell Count
MH  - Chronic Disease
MH  - Eosinophils/*immunology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/*immunology
MH  - Neutrophil Activation
MH  - Neutrophils/*immunology
MH  - Proteome
MH  - Rhinitis/*immunology
MH  - Signal Transduction
MH  - Sinusitis/*immunology
MH  - Th2 Cells/*immunology
MH  - Triggering Receptor Expressed on Myeloid Cells-1/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - chronic rhinosinusitis
OT  - eosinophil
OT  - nasal polyps
OT  - neutrophil
OT  - proteomic
EDAT- 2018/07/03 06:00
MHDA- 2019/01/12 06:00
CRDT- 2018/07/03 06:00
PHST- 2018/07/03 06:00 [pubmed]
PHST- 2019/01/12 06:00 [medline]
PHST- 2018/07/03 06:00 [entrez]
AID - 10.1177/1945892418782233 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2018 Sep;32(5):359-368. doi: 10.1177/1945892418782233. Epub 
      2018 Jul 2.