PMID- 29963009
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1664-2295 (Print)
IS  - 1664-2295 (Electronic)
IS  - 1664-2295 (Linking)
VI  - 9
DP  - 2018
TI  - Umbilical Cord-Derived Mesenchymal Stromal Cells Contribute to Neuroprotection in 
      Neonatal Cortical Neurons Damaged by Oxygen-Glucose Deprivation.
PG  - 466
LID - 10.3389/fneur.2018.00466 [doi]
LID - 466
AB  - Several studies have reported that human umbilical cord-derived mesenchymal 
      stromal cells (UC-MSCs) restore neurological damage in vivo through their 
      secretion of paracrine factors. We previously found that UC-MSCs attenuate brain 
      injury by secreting neurotrophic factors, such as brain-derived neurotrophic 
      factor (BDNF) and hepatocyte growth factor (HGF). However, how these factors 
      contribute to neuroprotection remains unknown. In this study, we aimed to 
      investigate to what extent UC-MSC-derived HGF and BDNF contribute to 
      neuroprotection using a Transwell co-culture system of neonatal cortical neurons 
      damaged by oxygen-glucose deprivation. The influence of HGF and BDNF were 
      determined by investigating neurons in both the presence and absence of UC-MSCs 
      as these cells consistently secrete both factors and can be blocked by 
      neutralizing antibodies. In the co-culture, UC-MSCs significantly improved 
      neuronal injury, as indicated by an increase in immature neuron number, neurite 
      outgrowth, and cell proliferation. Co-culture of damaged neurons with UC-MSCs 
      also exhibited a reduction in the number of neurons displaying signs of 
      apoptosis/necrosis. The neuroprotective actions of UC-MSCs were partially 
      reverted by neutralizing antibodies. Together, our findings reveal that 
      UC-MSC-secreted HGF and BDNF have neuroprotective effects on damaged neurons. 
      Further studies should address the existence of other potential neurotrophic 
      paracrine factors.
FAU - Mukai, Takeo
AU  - Mukai T
AD  - Division of Molecular of Therapy, Center for Advanced Medical Research, Institute 
      of Medical Science, University of Tokyo, Tokyo, Japan.
AD  - Department of Cell Processing and Transfusion, Institute of Medical Science, 
      University of Tokyo, Tokyo, Japan.
FAU - Tojo, Arinobu
AU  - Tojo A
AD  - Division of Molecular of Therapy, Center for Advanced Medical Research, Institute 
      of Medical Science, University of Tokyo, Tokyo, Japan.
AD  - Department of Cell Processing and Transfusion, Institute of Medical Science, 
      University of Tokyo, Tokyo, Japan.
FAU - Nagamura-Inoue, Tokiko
AU  - Nagamura-Inoue T
AD  - Department of Cell Processing and Transfusion, Institute of Medical Science, 
      University of Tokyo, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180615
PL  - Switzerland
TA  - Front Neurol
JT  - Frontiers in neurology
JID - 101546899
PMC - PMC6013549
OTO - NOTNLM
OT  - brain derived neurotrophic factor
OT  - cerebral palsy
OT  - hepatocyte growth factor
OT  - mesenchymal stromal cell
OT  - neonatal encephalopathy
OT  - umbilical cord
EDAT- 2018/07/03 06:00
MHDA- 2018/07/03 06:01
PMCR- 2018/06/15
CRDT- 2018/07/03 06:00
PHST- 2017/11/10 00:00 [received]
PHST- 2018/05/31 00:00 [accepted]
PHST- 2018/07/03 06:00 [entrez]
PHST- 2018/07/03 06:00 [pubmed]
PHST- 2018/07/03 06:01 [medline]
PHST- 2018/06/15 00:00 [pmc-release]
AID - 10.3389/fneur.2018.00466 [doi]
PST - epublish
SO  - Front Neurol. 2018 Jun 15;9:466. doi: 10.3389/fneur.2018.00466. eCollection 2018.