PMID- 29963124
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 6
DP  - 2018 Jun
TI  - SB431542 inhibited cigarette smoke extract induced invasiveness of A549 cells via 
      the TGF-beta1/Smad2/MMP3 pathway.
PG  - 9681-9686
LID - 10.3892/ol.2018.8556 [doi]
AB  - Lung cancer has high morbidity and mortality rates. Smoking is involved in the 
      pathogenesis of lung cancer, and tobacco smoke may increase tumor cell invasion 
      and metastasis. The effects of cigarette smoke extract (CSE) on the carcinoma 
      human alveolar basal epithelial A549 cell line were investigated. A549 cells were 
      exposed to increasing concentrations of CSE for 12, 24 and 48 h, and the 
      transforming growth factor-beta1 (TGF-beta1) signal pathway was inhibited by addition 
      of SB431542, a TGF-beta1 receptor antagonist. The proliferation of A549 cells was 
      assayed by a Cell Counting kit-8, invasiveness was assayed using Transwell 
      chambers, and TGF-beta1, phosphorylated mothers against decapentaplegic homolog 2 
      (p-Smad2), and matrix metalloproteinase 3 (MMP3) levels was assessed by western 
      blot analysis. The invasiveness of A549 cells and the expression of TGF-beta1, 
      pSmad2, and MMP-3 were significantly increased by CSE (P<0.05). The effects of 
      CSE were abrogated by SB431542 (P<0.05). In conclusion, CSE increased the 
      invasiveness of A549 cells and its effects were abrogated by SB431542 and the 
      TGF-beta1/Smad2/MMP-3 pathway may have been involved.
FAU - Liao, Ke
AU  - Liao K
AD  - Department of Respiration and Gastroenterology, The Seventh Peoples Hospital of 
      Chengdu, Chengdu, Sichuan 610000, P.R. China.
FAU - Yong, Chen-Wei
AU  - Yong CW
AD  - Department of Respiration and Gastroenterology, The Seventh Peoples Hospital of 
      Chengdu, Chengdu, Sichuan 610000, P.R. China.
FAU - Hua, Ke
AU  - Hua K
AD  - Department of Respiration and Gastroenterology, The Seventh Peoples Hospital of 
      Chengdu, Chengdu, Sichuan 610000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180423
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6020173
OTO - NOTNLM
OT  - A549 cells
OT  - SB431542
OT  - cigarette smoke extract
OT  - matrix metalloproteinase 3
OT  - mothers against decapentaplegic homolog 2
OT  - transforming growth factor-beta1
EDAT- 2018/07/03 06:00
MHDA- 2018/07/03 06:01
PMCR- 2018/04/23
CRDT- 2018/07/03 06:00
PHST- 2017/08/27 00:00 [received]
PHST- 2018/02/19 00:00 [accepted]
PHST- 2018/07/03 06:00 [entrez]
PHST- 2018/07/03 06:00 [pubmed]
PHST- 2018/07/03 06:01 [medline]
PHST- 2018/04/23 00:00 [pmc-release]
AID - OL-0-0-8556 [pii]
AID - 10.3892/ol.2018.8556 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Jun;15(6):9681-9686. doi: 10.3892/ol.2018.8556. Epub 2018 Apr 
      23.