PMID- 29964895
OWN - NLM
STAT- MEDLINE
DCOM- 20181012
LR  - 20221222
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 37
IP  - 6
DP  - 2016 Jun 8
TI  - [Optimization for Microthrix parvicella Quantitative Processing of Fluorescence 
      in situ Hybridization (FISH)].
PG  - 2266-2270
LID - 10.13227/j.hjkx.2016.06.033 [doi]
AB  - Precise quantification of Microthrix parvicella, which is identified as a 
      dominated filamentous bacterium of bulking sludge in the worldwide, is essential 
      for bulking investigation and related control strategies. However, quantitative 
      processing based on fluorescence in situ hybridization (FISH) is prone to 
      interference due to the specific characteristics of Microthrix parvicella 
      (hydrophobic surface with thick cell wall). Our study focused on pretreatment and 
      process optimization to show that the proportion of Microthrix parvicella was 
      increased from 1.12% to 96.70% benefited by lysozyme (36000 U.mL(-1)), high probe 
      concentration (4.5 ng.muL(-1)) and longer hybridization time (4 h) employed, 
      mapping with the results of q-PCR method and Eikelboom & Jenkins Observation.
FAU - Wang, Run-Fang
AU  - Wang RF
AD  - Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 
      Beijing 100085, China.
AD  - Water Quality Testing Center, Beijing Drainage Water Environment Development Co., 
      Ltd., Beijing 100022, China.
FAU - Zhang, Hong
AU  - Zhang H
AD  - Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 
      Beijing 100085, China.
FAU - Wang, Qin
AU  - Wang Q
AD  - College of Chemistry and Environmental Science, Hebei University, Baoding 071002, 
      China.
FAU - Wang, Juan
AU  - Wang J
AD  - Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 
      Beijing 100085, China.
FAU - Gu, Jian
AU  - Gu J
AD  - Water Quality Testing Center, Beijing Drainage Water Environment Development Co., 
      Ltd., Beijing 100022, China.
FAU - Qi, Rong
AU  - Qi R
AD  - Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 
      Beijing 100085, China.
FAU - Yang, Min
AU  - Yang M
AD  - Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 
      Beijing 100085, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Sewage)
SB  - IM
MH  - Actinobacteria/*isolation & purification
MH  - Hydrophobic and Hydrophilic Interactions
MH  - *In Situ Hybridization, Fluorescence
MH  - Sewage/*microbiology
OTO - NOTNLM
OT  - Microthrix parvicella
OT  - fluorescence in situ hybridization(FISH)
OT  - optimization
OT  - quantitative
OT  - sludge bulking
EDAT- 2016/06/08 00:00
MHDA- 2016/06/08 00:01
CRDT- 2018/07/03 06:00
PHST- 2018/07/03 06:00 [entrez]
PHST- 2016/06/08 00:00 [pubmed]
PHST- 2016/06/08 00:01 [medline]
AID - 10.13227/j.hjkx.2016.06.033 [doi]
PST - ppublish
SO  - Huan Jing Ke Xue. 2016 Jun 8;37(6):2266-2270. doi: 10.13227/j.hjkx.2016.06.033.