PMID- 29965219 OWN - NLM STAT- MEDLINE DCOM- 20181211 LR - 20181211 IS - 0250-3301 (Print) IS - 0250-3301 (Linking) VI - 38 IP - 10 DP - 2017 Oct 8 TI - [Effect of Two-Stage Aeration on Nitrogen Removal Performance of Aerobic Granular Sludge]. PG - 4332-4339 LID - 10.13227/j.hjkx.201703207 [doi] AB - Four mini experiments were conducted at different conditions. The heterotrophic microorganisms on the aerobic granular sludge surface consumed organic compounds at the initial stage of aeration. The denitrification rate and the efficiency of NO(2)(-)-N and NO(3)(-)-N removal were relatively low. Therefore, under the normal temperature conditions (20-23℃), aerobic granular sludge sequencing batch reactor (SBR) was operated in the two-stage aeration mode(first in low aeration then in high aeration mode). The low aeration time were carried out at 1, 2 and 3 hours stages respectively, and the characteristics of the granular sludge and its effects on microorganisms were analyzed by scanning electron microscopy (SEM) and fluorescence in situ hybridization (FISH) technique. The results show that the increase in the aerobic granular sludge (AGS) particle size improved the denitrification capacity; the denitrification rate of NO(2)(-)-N was the highest at low aeration mode with 2 h and reached 9.66 mg.(g.h)(-1). The accumulation rate of nitrite increased to 77.84% and the total nitrogen removal rate to 70%. The bacterial count inside the granular sludge increased and they were mainly cocci, bacillus, and ellipsoidal bacteria. Moreover, the proportion of ammonia-oxidizing bacteria in total bacterial count increased from 13.70% to 15.40%. Therefore, the two-stage aeration process achieved shortened simultaneous nitrification and denitrification processes and showed a good denitrification performance. FAU - Wang, Wen-Xiao AU - Wang WX AD - College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China. FAU - Bian, Wei AU - Bian W AD - College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China. FAU - Wang, Meng AU - Wang M AD - Beijing Drainage Group Co., Ltd., Beijing 100044, China. FAU - Kan, Rui-Zhe AU - Kan RZ AD - College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China. FAU - Zhao, Qing AU - Zhao Q AD - College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China. FAU - Liang, Dong-Bo AU - Liang DB AD - College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China. FAU - Li, Jun AU - Li J AD - College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China. LA - chi PT - Journal Article PL - China TA - Huan Jing Ke Xue JT - Huan jing ke xue= Huanjing kexue JID - 8405344 RN - 0 (Sewage) RN - N762921K75 (Nitrogen) SB - IM MH - Bacteria/classification MH - *Bioreactors MH - *Denitrification MH - In Situ Hybridization, Fluorescence MH - Nitrogen/*isolation & purification MH - Sewage/*microbiology MH - *Waste Disposal, Fluid OTO - NOTNLM OT - aerobic granular sludge OT - denitrification rate OT - nitrite accumulation rate OT - shortcut simultaneous nitrification and denitrification OT - two-stage aeration EDAT- 2018/07/03 06:00 MHDA- 2018/12/12 06:00 CRDT- 2018/07/03 06:00 PHST- 2018/07/03 06:00 [entrez] PHST- 2018/07/03 06:00 [pubmed] PHST- 2018/12/12 06:00 [medline] AID - 10.13227/j.hjkx.201703207 [doi] PST - ppublish SO - Huan Jing Ke Xue. 2017 Oct 8;38(10):4332-4339. doi: 10.13227/j.hjkx.201703207.