PMID- 29965474
OWN - NLM
STAT- MEDLINE
DCOM- 20190115
LR  - 20190115
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 39
IP  - 3
DP  - 2018 Mar 8
TI  - [Characteristics of Biofilm During the Transition Process of Complete 
      Nitrification and Partial Nitrification].
PG  - 1278-1285
LID - 10.13227/j.hjkx.201708055 [doi]
AB  - The objective of the study was to investigate the change of biofilm 
      characteristics when implementing the procedure of partial nitrification. A ratio 
      control strategy (DO/NH(4)(+)-N) was taken to achieve partial nitrification, and 
      biofilm samples were obtained at 10.27%, 52.12%, and 93.54% of the nitrite 
      accumulation rate. The amount and spatial distribution of total bacteria, ammonia 
      oxidizing bacteria (AOB), and nitrite oxidative bacteria (NOB) were observed by 
      fluorescence in situ hybridization (FISH) and confocal laser scanning microscope 
      (CLSM) through a three-dimensional excitation emission matrix (EEM) to observe 
      the secretion and composition changes of extracellular polymer substances. Ratio 
      control successfully enriched AOB and achieved partial nitrification under 
      conditions when NOB was not completely washed. Heterotrophic bacteria and 
      nitrifying bacteria coexist in the biofilm. The heterotrophic bacteria were in 
      the outer layer, but nitrifying bacteria were distributed in the biofilm surface 
      at 6-25 mum. During the process of short-range nitrification, the AOB/NOB value 
      gradually increased, and the stable operation period was as high as 15.56. During 
      the operation of the reactor, EPS and microbial flora changes are closely 
      related. When microbial activity decreased, EPS secretion decreased. During the 
      stable operation period of partial nitrification, NOB and other bacteria that are 
      non-resistant to high nitrite nitrous acid declined, and the fluorescence 
      intensity of aromatic protein-like bacteria decreased. However, the 
      three-dimensional fluorescence spectra showed that the chemical composition of 
      EPS was not obvious during the process of partial nitrification.
FAU - Zhao, Qing
AU  - Zhao Q
AD  - College of Architecture and Civil Engineering, Beijing University of Technology, 
      Beijing 100124, China.
FAU - Bian, Wei
AU  - Bian W
AD  - College of Architecture and Civil Engineering, Beijing University of Technology, 
      Beijing 100124, China.
FAU - Li, Jun
AU  - Li J
AD  - College of Architecture and Civil Engineering, Beijing University of Technology, 
      Beijing 100124, China.
FAU - Wang, Wen-Xiao
AU  - Wang WX
AD  - College of Architecture and Civil Engineering, Beijing University of Technology, 
      Beijing 100124, China.
FAU - Sun, Yi-Qi
AU  - Sun YQ
AD  - College of Architecture and Civil Engineering, Beijing University of Technology, 
      Beijing 100124, China.
FAU - Liang, Dong-Bo
AU  - Liang DB
AD  - College of Architecture and Civil Engineering, Beijing University of Technology, 
      Beijing 100124, China.
FAU - Zhang, Shu-Yan
AU  - Zhang SY
AD  - China Energy Saving and Emission Reduction Limited Company of Shenhua Group, 
      Beijing 100011, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia
MH  - Bacteria/metabolism
MH  - *Biofilms
MH  - *Bioreactors
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - *Nitrification
MH  - Nitrites/*isolation & purification
OTO - NOTNLM
OT  - biofilm
OT  - extracellular polymeric substances
OT  - nitrifying bacteria
OT  - partial nitrification
EDAT- 2018/07/03 06:00
MHDA- 2019/01/16 06:00
CRDT- 2018/07/03 06:00
PHST- 2018/07/03 06:00 [entrez]
PHST- 2018/07/03 06:00 [pubmed]
PHST- 2019/01/16 06:00 [medline]
AID - 10.13227/j.hjkx.201708055 [doi]
PST - ppublish
SO  - Huan Jing Ke Xue. 2018 Mar 8;39(3):1278-1285. doi: 10.13227/j.hjkx.201708055.