PMID- 29967092
OWN - NLM
STAT- MEDLINE
DCOM- 20190305
LR  - 20211204
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 86
IP  - 9
DP  - 2018 Sep
TI  - The Protozoan Parasite Toxoplasma gondii Selectively Reprograms the Host Cell 
      Translatome.
LID - 10.1128/IAI.00244-18 [doi]
LID - e00244-18
AB  - The intracellular parasite Toxoplasma gondii promotes infection by targeting 
      multiple host cell processes; however, whether it modulates mRNA translation is 
      currently unknown. Here, we show that infection of primary murine macrophages 
      with type I or II T. gondii strains causes a profound perturbation of the host 
      cell translatome. Notably, translation of transcripts encoding proteins involved 
      in metabolic activity and components of the translation machinery was activated 
      upon infection. In contrast, the translational efficiency of mRNAs related to 
      immune cell activation and cytoskeleton/cytoplasm organization was largely 
      suppressed. Mechanistically, T. gondii bolstered mechanistic target of rapamycin 
      (mTOR) signaling to selectively activate the translation of mTOR-sensitive mRNAs, 
      including those with a 5'-terminal oligopyrimidine (5' TOP) motif and those 
      encoding mitochondrion-related proteins. Consistent with parasite modulation of 
      host mTOR-sensitive translation to promote infection, inhibition of mTOR activity 
      suppressed T. gondii replication. Thus, selective reprogramming of host mRNA 
      translation represents an important subversion strategy during T. gondii 
      infection.
CI  - Copyright (c) 2018 American Society for Microbiology.
FAU - Leroux, Louis-Philippe
AU  - Leroux LP
AUID- ORCID: 0000-0002-9736-1827
AD  - Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, 
      Quebec, Canada.
AD  - Centre for Host-Parasite Interactions, Institut National de la Recherche 
      Scientifique-Institut Armand Frappier, Laval, Quebec, Canada.
FAU - Lorent, Julie
AU  - Lorent J
AD  - Department of Oncology-Pathology, Science for Life Laboratory, Karolinska 
      Institutet, Stockholm, Sweden.
FAU - Graber, Tyson E
AU  - Graber TE
AD  - Children's Hospital of Eastern Ontario Research Institute, Department of 
      Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, 
      Canada.
FAU - Chaparro, Visnu
AU  - Chaparro V
AD  - Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, 
      Quebec, Canada.
AD  - Centre for Host-Parasite Interactions, Institut National de la Recherche 
      Scientifique-Institut Armand Frappier, Laval, Quebec, Canada.
FAU - Masvidal, Laia
AU  - Masvidal L
AD  - Department of Oncology-Pathology, Science for Life Laboratory, Karolinska 
      Institutet, Stockholm, Sweden.
FAU - Aguirre, Maria
AU  - Aguirre M
AD  - Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada.
FAU - Fonseca, Bruno D
AU  - Fonseca BD
AD  - Children's Hospital of Eastern Ontario Research Institute, Department of 
      Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, 
      Canada.
FAU - van Kempen, Leon C
AU  - van Kempen LC
AD  - Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada.
AD  - Department of Pathology, McGill University, Montreal, Quebec, Canada.
AD  - Department of Pathology and Medical Biology, University Medical Centre, 
      Groningen, The Netherlands.
FAU - Alain, Tommy
AU  - Alain T
AD  - Children's Hospital of Eastern Ontario Research Institute, Department of 
      Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, 
      Canada.
FAU - Larsson, Ola
AU  - Larsson O
AD  - Department of Oncology-Pathology, Science for Life Laboratory, Karolinska 
      Institutet, Stockholm, Sweden.
FAU - Jaramillo, Maritza
AU  - Jaramillo M
AUID- ORCID: 0000-0002-1910-5684
AD  - Institut National de la Recherche Scientifique-Institut Armand Frappier, Laval, 
      Quebec, Canada maritza.jaramillo@iaf.inrs.ca.
AD  - Centre for Host-Parasite Interactions, Institut National de la Recherche 
      Scientifique-Institut Armand Frappier, Laval, Quebec, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180822
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Protozoan Proteins)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Host-Parasite Interactions
MH  - Macrophages/*parasitology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitochondrial Proteins/genetics
MH  - Protein Biosynthesis/*genetics
MH  - Protozoan Proteins/immunology
MH  - RNA 5' Terminal Oligopyrimidine Sequence
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics
MH  - Toxoplasma/*pathogenicity
PMC - PMC6105892
OTO - NOTNLM
OT  - Toxoplasma gondii
OT  - host-pathogen interactions
OT  - mTOR
OT  - macrophages
OT  - translational control
EDAT- 2018/07/04 06:00
MHDA- 2019/03/06 06:00
PMCR- 2019/02/22
CRDT- 2018/07/04 06:00
PHST- 2018/03/29 00:00 [received]
PHST- 2018/06/22 00:00 [accepted]
PHST- 2018/07/04 06:00 [pubmed]
PHST- 2019/03/06 06:00 [medline]
PHST- 2018/07/04 06:00 [entrez]
PHST- 2019/02/22 00:00 [pmc-release]
AID - IAI.00244-18 [pii]
AID - 00244-18 [pii]
AID - 10.1128/IAI.00244-18 [doi]
PST - epublish
SO  - Infect Immun. 2018 Aug 22;86(9):e00244-18. doi: 10.1128/IAI.00244-18. Print 2018 
      Sep.