PMID- 29967803
OWN - NLM
STAT- MEDLINE
DCOM- 20181018
LR  - 20181114
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2018
DP  - 2018
TI  - Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the 
      Functional Activity of Dendritic Cells via Formyl Peptide Receptors.
PG  - 7873257
LID - 10.1155/2018/7873257 [doi]
LID - 7873257
AB  - The Crotalus durissus terrificus rattlesnake venom, its main toxin, crotoxin 
      (CTX), and its crotapotin (CA) and phospholipase A(2) (CB) subunits modulate the 
      immune system. Formyl peptide receptors (FPRs) and lipoxin A(4) (LXA(4)) are 
      involved in CTX's effect on macrophages and neutrophils. Dendritic cells (DCs) 
      are plasticity cells involved in the induction of adaptive immunity and tolerance 
      maintenance. Therefore, we evaluated the effect of CTX, CA or CB on the 
      maturation of DCs derived from murine bone marrow (BM). According to data, CTX 
      and CB-but not CA-induced an increase of MHC-II, but not costimulatory molecules 
      on DCs. Furthermore, CTX and CB inhibited the expression of costimulatory and 
      MHC-II molecules, secretion of proinflammatory cytokines and NF-kappaBp65 and 
      p38/ERK1/2-MAPK signaling pathways by LPS-incubated DCs. Differently, CTX and CB 
      induced IL-10, PGE(2) and LXA(4) secretion in LPS-incubated DCs. Lower 
      proliferation and IL-2 secretion were verified in coculture of CD3(+) cells and 
      DCs incubated with LPS plus CTX or CB compared with LPS-incubated DCs. The effect 
      of CTX and CB on DCs was abolished in cultures incubated with a FPRs antagonist. 
      Hence, CTX and CB exert a modulation on functional activity of DCs; we also 
      checked the involvement the FPR family on cell activities.
FAU - Freitas, A P
AU  - Freitas AP
AD  - Immunopathology Laboratory, Butantan Institute, Sao Paulo, SP, Brazil.
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, SP, Brazil.
FAU - Favoretto, B C
AU  - Favoretto BC
AD  - Immunopathology Laboratory, Butantan Institute, Sao Paulo, SP, Brazil.
FAU - Clissa, P B
AU  - Clissa PB
AD  - Immunopathology Laboratory, Butantan Institute, Sao Paulo, SP, Brazil.
FAU - Sampaio, S C
AU  - Sampaio SC
AUID- ORCID: 0000-0001-6257-3531
AD  - Physiopathology Laboratory, Butantan Institute, Sao Paulo, SP, Brazil.
AD  - Department of Pharmacology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, SP, Brazil.
FAU - Faquim-Mauro, E L
AU  - Faquim-Mauro EL
AUID- ORCID: 0000-0001-8083-4272
AD  - Immunopathology Laboratory, Butantan Institute, Sao Paulo, SP, Brazil.
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20180603
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 9007-40-3 (Crotoxin)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Crotoxin/*pharmacology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*drug effects/immunology/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Histocompatibility Antigens Class II/genetics
MH  - Immunomodulation/*drug effects
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides/immunology
MH  - Male
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Phosphorylation
MH  - Receptors, Formyl Peptide/*metabolism
MH  - Signal Transduction/drug effects
MH  - T-Lymphocyte Subsets/immunology/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
PMC - PMC6008858
EDAT- 2018/07/04 06:00
MHDA- 2018/10/20 06:00
PMCR- 2018/06/03
CRDT- 2018/07/04 06:00
PHST- 2017/12/13 00:00 [received]
PHST- 2018/04/08 00:00 [accepted]
PHST- 2018/07/04 06:00 [entrez]
PHST- 2018/07/04 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
PHST- 2018/06/03 00:00 [pmc-release]
AID - 10.1155/2018/7873257 [doi]
PST - epublish
SO  - J Immunol Res. 2018 Jun 3;2018:7873257. doi: 10.1155/2018/7873257. eCollection 
      2018.