PMID- 29974427
OWN - NLM
STAT- MEDLINE
DCOM- 20190315
LR  - 20221207
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1810
DP  - 2018
TI  - LC-MS-MS Method Development and Analysis of Stimulants, Opiates, Synthetic 
      Opiates, PCP, and Benzodiazepines in Wastewater. Preponderance of these Drugs 
      During Football Games.
PG  - 149-182
LID - 10.1007/978-1-4939-8579-1_15 [doi]
AB  - A method was developed for the analysis of stimulant drugs, opiates, synthetic 
      opiates, PCP, and benzodiazepines in wastewater samples using liquid 
      chromatography coupled with tandem mass spectrometry (LC-MS-MS). A total of 33 
      compounds (stimulant-type drugs and metabolites of opiates, synthetic opiates, 
      PCP, and benzodiazepines) were analyzed. These drugs included amphetamine (Amp) 
      (1), methamphetamine (Meth) (2), methylenedioxyamphetamine (MDA) (3), 
      methylenedioxymethamphetamine (MDMA) (4), methylenedioxyethylamphetamine (MDEA) 
      (5), benzoylecgonine (BE, the major metabolite of Coc) (6), cocaine (Coc) (7), 
      6-monoacetylmorphine (6-MAM, the primary urinary metabolite of heroin) (8), 
      codeine (9), hydrocodone (10), hydromorphone (11), morphine (12), norhydrocodone 
      (the primary urinary metabolite of hydrocodone) (13), oxycodone (14), oxymorphone 
      (15), 2-ethylidine-1,5-dimethyl-3,3-diphenylpyrolidine (EDDP, the primary urinary 
      metabolite of methadone) (16), fentanyl (17), meperidine (18), methadone (19), 
      norfentanyl (the primary urinary metabolite of fentanyl) (20), normeperidine (the 
      primary urinary metabolite of meperidine) (21), phencyclidine (PCP) (22), 
      tramadol (23), alprazolam (24), temazepam (25), nordiazepam (26), 
      chlordiazepoxide (27), flurazepam (28), oxazepam (29), alpha-OH-alprazolam (the 
      primary urinary metabolite of alprazolam) (30), alpha-OH-triazolam (the primary 
      urinary metabolite of triazolam) (31), 2-OH-ethylflurazepam (the primary urinary 
      metabolite of flurazepam) (32), and 7-NH(2)-flunitrazepam (the primary urinary 
      metabolite of flunitrazepam) (33). These drugs were chosen because of their 
      widespread abuse. Wastewater samples were collected at both the Oxford Wastewater 
      Treatment Plant in Oxford, Mississippi (MS), and the University Wastewater 
      Treatment Plant in University, MS. Samples were collected on weekends on which 
      the Ole Miss Rebel football team held home games (Vaught-Hemingway Stadium, 
      University, MS 38677). The collected samples were analyzed using a validated 
      method and found to contain Amp, Meth, MDMA, MDA, Coc, BE, codeine, hydrocodone, 
      hydromorphone, morphine, norhydrocodone, oxycodone, oxymorphone, tramadol, EDDP, 
      meperidine, normeperidine, methadone, alprazolam, alpha-OH-alprazolam, nordiazepam, 
      oxazepam, and temazepam. None of the samples contained MDEA, 6-MAM, fentanyl, 
      norfentanyl, PCP, chlordiazepoxide, flurazepam, 2-OH-ethylflurazepam, 
      7-NH(2)-flunitrazepam, and alpha-OH-triazolam.
FAU - Gul, Waseem
AU  - Gul W
AD  - ElSohly Laboratories, Inc., Oxford, MS, USA.
AD  - National Center for Natural Products Research, University of Mississippi, 
      University, MS, USA.
FAU - Gul, Shahbaz W
AU  - Gul SW
AD  - ElSohly Laboratories, Inc., Oxford, MS, USA.
FAU - Stamper, Brandon
AU  - Stamper B
AD  - ElSohly Laboratories, Inc., Oxford, MS, USA.
AD  - Department of Chemistry and Biochemistry, University of Mississippi, University, 
      MS, USA.
FAU - Godfrey, Murrell
AU  - Godfrey M
AD  - Department of Chemistry and Biochemistry, University of Mississippi, University, 
      MS, USA.
FAU - ElSohly, Mahmoud A
AU  - ElSohly MA
AD  - ElSohly Laboratories, Inc., Oxford, MS, USA. elsohly@elsohly.com.
AD  - National Center for Natural Products Research, University of Mississippi, 
      University, MS, USA. elsohly@elsohly.com.
AD  - Department of Pharmaceutics and Drug Delivery, University of Mississippi, 
      University, MS, USA. elsohly@elsohly.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Opiate Alkaloids)
RN  - 0 (Waste Water)
RN  - 12794-10-4 (Benzodiazepines)
SB  - IM
MH  - Benzodiazepines/*analysis
MH  - Central Nervous System Stimulants/*analysis
MH  - *Chromatography, Liquid
MH  - Data Analysis
MH  - Humans
MH  - Opiate Alkaloids/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - *Substance Abuse Detection/methods
MH  - Substance-Related Disorders/*epidemiology
MH  - *Tandem Mass Spectrometry
MH  - Wastewater/*analysis
OTO - NOTNLM
OT  - Benzodiazepines
OT  - LC-MS/MS
OT  - Opiates
OT  - Stimulant drugs
OT  - Wastewater
EDAT- 2018/07/06 06:00
MHDA- 2019/03/16 06:00
CRDT- 2018/07/06 06:00
PHST- 2018/07/06 06:00 [entrez]
PHST- 2018/07/06 06:00 [pubmed]
PHST- 2019/03/16 06:00 [medline]
AID - 10.1007/978-1-4939-8579-1_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1810:149-182. doi: 10.1007/978-1-4939-8579-1_15.