PMID- 29975807
OWN - NLM
STAT- MEDLINE
DCOM- 20191111
LR  - 20191111
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Linking)
VI  - 48
IP  - 9
DP  - 2018 Sep
TI  - Towards complete identification of allergens in Jack Jumper (Myrmecia pilosula) 
      ant venom and their clinical relevance: An immunoproteomic approach.
PG  - 1222-1234
LID - 10.1111/cea.13224 [doi]
AB  - BACKGROUND: The venomous stings of Jack Jumper ant (JJA; species of the Myrmecia 
      pilosula taxonomic group) are a significant public health issue in parts of 
      south-eastern and south-western Australia, causing anaphylaxis in approximately 
      3% of the population. Three allergenic peptides, Myr p 1, Myr p 2 and Myr p 3, 
      and one histamine-releasing peptide, pilosulin 5, have been fully described, but 
      there are at least 5 additional high molecular weight IgE-binding components that 
      have not been identified. OBJECTIVE: To identify IgE-binding components in JJA 
      venom (JJAV) and to relate the IgE recognition of these components to relevant 
      clinical parameters. METHODS: Identification of IgE-binding components and 
      determination of their sensitizing prevalence was performed using SDS-PAGE 
      immunoblot assay and sera from 90 patients with confirmed allergy to JJAV. Tandem 
      mass spectrometry was used for identification of novel JJAV components 
      fractionated by size exclusion chromatography (SEC) and SDS-PAGE. RESULTS: Using 
      SDS-PAGE immunoblot, 10 IgE-binding bands were identified in JJAV, two of which 
      were recognized by 81% and 47% of the population studied. Mass spectrometry 
      identified 17 novel JJAV proteins, including 2 glycoproteins, and confirmed the 
      presence of 4 known Myr p and pilosulin peptides in JJAV. Most of the newly 
      identified IgE-binding proteins were enzymes, including phospholipase A(2) , 
      hyaluronidase, arginine kinase and dipeptidyl peptidase IV. Correlations were 
      found between recognition of certain IgE-binding bands with JJAV-specific IgE 
      titre by ImmunoCAP, intradermal test threshold and treatment-related issues. 
      CONCLUSIONS AND CLINICAL RELEVANCE: This study has for the first time revealed 
      the identity of various proteins with IgE-binding capacity in the venom of JJA 
      and demonstrated their clinical relevance in the diagnosis and treatment of JJAV 
      allergy.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Wanandy, Troy
AU  - Wanandy T
AUID- ORCID: 0000-0003-4703-0488
AD  - Jack Jumper Allergy Program, Royal Hobart Hospital, Hobart, TAS, Australia.
AD  - Division of Pharmacy, School of Medicine, University of Tasmania, Hobart, TAS, 
      Australia.
AD  - School of Medicine, University of Tasmania, Hobart, TAS, Australia.
AD  - Department of Pharmacy, Royal Hobart Hospital, Hobart, TAS, Australia.
FAU - Wilson, Richard
AU  - Wilson R
AUID- ORCID: 0000-0003-0152-4394
AD  - Central Science Laboratory, University of Tasmania, Hobart, TAS, Australia.
FAU - Gell, David
AU  - Gell D
AD  - School of Medicine, University of Tasmania, Hobart, TAS, Australia.
FAU - Rose, Hayley E
AU  - Rose HE
AD  - Jack Jumper Allergy Program, Royal Hobart Hospital, Hobart, TAS, Australia.
FAU - Gueven, Nuri
AU  - Gueven N
AD  - Division of Pharmacy, School of Medicine, University of Tasmania, Hobart, TAS, 
      Australia.
FAU - Davies, Noel W
AU  - Davies NW
AD  - Central Science Laboratory, University of Tasmania, Hobart, TAS, Australia.
FAU - Brown, Simon G A
AU  - Brown SGA
AD  - Jack Jumper Allergy Program, Royal Hobart Hospital, Hobart, TAS, Australia.
AD  - School of Medicine, University of Tasmania, Hobart, TAS, Australia.
AD  - Ambulance Tasmania, Hobart, TAS, Australia.
AD  - Department of Emergency Medicine, Royal Hobart Hospital, Hobart, TAS, Australia.
FAU - Wiese, Michael D
AU  - Wiese MD
AD  - Jack Jumper Allergy Program, Royal Hobart Hospital, Hobart, TAS, Australia.
AD  - School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, 
      SA, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180803
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Ant Venoms)
RN  - 0 (Insect Proteins)
RN  - 0 (Peptides)
RN  - 0 (Proteome)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Allergens/chemistry/*immunology
MH  - Ant Venoms/*immunology
MH  - Antibody Specificity/immunology
MH  - *Epitope Mapping/methods
MH  - Female
MH  - Glycosylation
MH  - Humans
MH  - Hypersensitivity/diagnosis/immunology
MH  - Immunoglobulin E/immunology
MH  - Insect Proteins/chemistry/*immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Weight
MH  - Peptides/chemistry/immunology
MH  - Protein Binding/immunology
MH  - *Proteome/immunology
MH  - *Proteomics/methods
MH  - Young Adult
OTO - NOTNLM
OT  - Myrmecia pilosula
OT  - Hymenoptera venom
OT  - allergen
OT  - immunoproteomics
EDAT- 2018/07/06 06:00
MHDA- 2019/11/12 06:00
CRDT- 2018/07/06 06:00
PHST- 2017/11/08 00:00 [received]
PHST- 2018/05/25 00:00 [revised]
PHST- 2018/06/20 00:00 [accepted]
PHST- 2018/07/06 06:00 [pubmed]
PHST- 2019/11/12 06:00 [medline]
PHST- 2018/07/06 06:00 [entrez]
AID - 10.1111/cea.13224 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2018 Sep;48(9):1222-1234. doi: 10.1111/cea.13224. Epub 2018 Aug 
      3.