PMID- 29976081
OWN - NLM
STAT- MEDLINE
DCOM- 20181022
LR  - 20181022
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 46
IP  - 5
DP  - 2018
TI  - Curcuminoids Induce Reactive Oxygen Species and Autophagy to Enhance Apoptosis in 
      Human Oral Cancer Cells.
PG  - 1145-1168
LID - 10.1142/S0192415X1850060X [doi]
AB  - Numerous studies support the use of herbal medicine or natural products for 
      chemotherapy in human cancers. Reports have associated curcumin (CUR), dimethoxy 
      curcumin (DMC) and bisdemethoxycurcumin (BDMC) with numerous biological 
      activities including anticancer activities, but no available information have 
      shown that these induced apoptotic cell death and autophagy in human oral cancer 
      cells. In the present study, we investigated the effect of CUR, DMC and BDMC on 
      the cell viability, apoptotic cell death, reactive oxygen species (ROS), 
      Ca[Formula: see text], mitochondria membrane potential (MMP) and caspase 
      activities using flow cytometry assay and autophagy by monodansylcadaverine (MDC) 
      and acridine orange (AO) staining in human oral cancer SAS cells. Results 
      indicated that CUR, DMC and BDMC decreased total viable cell number through the 
      induction of cell autophagy and apoptosis in SAS cells. Cells were pretreated 
      with N-acetyl-cysteine (NAC), 3-methyladenine (3MA), rapamycin and 
      carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoro-methylketone (Z-VAD-fmk) and 
      then were treated with CUR, DMC and BDMC that led to increased total viable cell 
      number when compared to CUR, DMC and BDMC treatments only. Results indicated 
      induced apoptotic cell death through ROS, mitochondria-dependent pathway and 
      induction of cell autophagy. Based on those observations, we suggest that CUR, 
      DMC and BDMC could be used as a potential anticancer agent in human oral cancer.
FAU - Hsiao, Yung-Ting
AU  - Hsiao YT
AD  - * Department of Biological Science and Technology, China Medical University, 
      Taichung, Taiwan.
FAU - Kuo, Chao-Lin
AU  - Kuo CL
AD  - dagger Department of Chinese Medicine Resources, China Medical University, Taichung, 
      Taiwan.
FAU - Chueh, Fu-Shin
AU  - Chueh FS
AD  -  paragraph sign Department of Food Nutrition and Health Biotechnology, Asia University, Wufeng, 
      Taichung, Taiwan.
FAU - Liu, Kuo-Ching
AU  - Liu KC
AD  - double dagger Department of Medical Laboratory Science and Biotechnology, China Medical 
      University, Taichung, Taiwan.
FAU - Bau, Da-Tian
AU  - Bau DT
AD  -  section sign Graduate Institute of Biomedical and Sciences, China Medical University, 
      Taichung, Taiwan.
AD  - ** Terry Fox Cancer Research Laboratory, China Medical University Hospital, 
      Taichung, Taiwan.
FAU - Chung, Jing-Gung
AU  - Chung JG
AD  - * Department of Biological Science and Technology, China Medical University, 
      Taichung, Taiwan.
AD  -  parallel Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20180705
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Reactive Oxygen Species)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/chemistry/*pharmacology
MH  - Apoptosis/drug effects
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Curcumin/chemistry/*pharmacology
MH  - Humans
MH  - Mitochondria/drug effects/metabolism
MH  - Mouth Neoplasms/drug therapy/metabolism/*physiopathology
MH  - Reactive Oxygen Species/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Bisdemethoxycurcumin (BDMC)
OT  - Curcumin (CUR)
OT  - Demethoxycurcumin (DMC)
OT  - Oral Cancer
OT  - Reactive Oxygen Species
OT  - SAS Cells
EDAT- 2018/07/07 06:00
MHDA- 2018/10/23 06:00
CRDT- 2018/07/07 06:00
PHST- 2018/07/07 06:00 [pubmed]
PHST- 2018/10/23 06:00 [medline]
PHST- 2018/07/07 06:00 [entrez]
AID - 10.1142/S0192415X1850060X [doi]
PST - ppublish
SO  - Am J Chin Med. 2018;46(5):1145-1168. doi: 10.1142/S0192415X1850060X. Epub 2018 
      Jul 5.