PMID- 29977297
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-8787 (Print)
IS  - 1687-8795 (Electronic)
IS  - 1687-8787 (Linking)
VI  - 2018
DP  - 2018
TI  - In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of 
      Poly(dimethylsiloxane) Cured under Low Pressure.
PG  - 7546986
LID - 10.1155/2018/7546986 [doi]
LID - 7546986
AB  - Single embryo culture is useful for assessing the developmental competence of an 
      embryo in detail. Recently, a device made of poly(dimethylsiloxane) (PDMS), which 
      is biocompatible and nontoxic, has been widely used for culture various types of 
      cells. However, PDMS plates are porous, causing the serious osmolality increment 
      of the medium (over 600 mOsm/kg from Day 4 to Day 7). Here, we report that curing 
      the PDMS under low pressure (LP-PDMS) greatly reduced the porosity, resulting in 
      a constant osmolality of the medium. The blastocyst rate of single bovine embryos 
      cultured with LP-PDMS microwell (MW) plates was the same as that of 
      group-cultured embryos (25 embryos/50 mul droplet; control, P>0.05). These results 
      indicate that MWs on a plate made of PDMS cured under low pressure can be 
      successfully used for individual embryo culture.
FAU - Iwamoto, Daisaku
AU  - Iwamoto D
AD  - Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, 
      Wakayama 649-6493, Japan.
FAU - Kato, Nobuhiro
AU  - Kato N
AD  - Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, 
      Wakayama 649-6493, Japan.
FAU - Taniguchi, Shunji
AU  - Taniguchi S
AD  - Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, 
      Wakayama 649-6493, Japan.
AD  - Wakayama Prefecture Livestock Station, 1 Mirozu Susami, Wakayam, Japan.
FAU - Taguchi, Yoshitomo
AU  - Taguchi Y
AD  - Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, 
      Wakayama 649-6493, Japan.
FAU - Kishi, Masao
AU  - Kishi M
AD  - University Farm, Kindai University, Aridagawa, Wakayama 643-0531, Japan.
FAU - Saeki, Kazuhiro
AU  - Saeki K
AUID- ORCID: 0000-0002-1831-3844
AD  - Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, 
      Wakayama 649-6493, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180606
PL  - United States
TA  - Int J Biomater
JT  - International journal of biomaterials
JID - 101519099
PMC - PMC6011063
EDAT- 2018/07/07 06:00
MHDA- 2018/07/07 06:01
PMCR- 2018/06/06
CRDT- 2018/07/07 06:00
PHST- 2018/02/09 00:00 [received]
PHST- 2018/05/06 00:00 [accepted]
PHST- 2018/07/07 06:00 [entrez]
PHST- 2018/07/07 06:00 [pubmed]
PHST- 2018/07/07 06:01 [medline]
PHST- 2018/06/06 00:00 [pmc-release]
AID - 10.1155/2018/7546986 [doi]
PST - epublish
SO  - Int J Biomater. 2018 Jun 6;2018:7546986. doi: 10.1155/2018/7546986. eCollection 
      2018.