PMID- 2997998
OWN - NLM
STAT- MEDLINE
DCOM- 19851212
LR  - 20200409
IS  - 0042-6822 (Print)
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 143
IP  - 1
DP  - 1985 May
TI  - Identification and characterization of a mouse mammary tumor virus protein 
      uniquely expressed on the surface of BALB/cV mammary tumor cells.
PG  - 127-42
AB  - A unique subline of BALB/c mice, designated BALB/cV, exhibits an intermediate 
      mammary tumor incidence (47%) and harbors a distinct milk-transmitted mouse 
      mammary tumor virus (MMTV). The BALB/cV subline was used to study the molecular 
      basis of potential virus-host interactions involving cell surface-expressed MMTV 
      proteins. Cell surface iodination identified virus-specific proteins expressed on 
      BALB/cV primary mammary tumor cells grown in culture. In contrast to 
      (C3H)MMTV-producing cell lines which expressed MMTV gp52, BALB/cV tumor cells 
      lacked gp52 and expressed instead a 68K, env-related protein. The 68Kenv protein 
      was also detected on the surface of metabolically labeled BALB/cV tumor cells by 
      an external immunoprecipitation technique. The expression of 68Kenv was 
      restricted to mammary tissues of BALB/cV mice that also expressed other MMTV 
      proteins. Biochemical analysis established that 68Kenv was not modified by 
      N-linked glycosylation. 125I-labeled 68Kenv was rapidly released into the media 
      of tumor cell cultures and was recovered both in the form of a soluble protein 
      and in a 100,000 g pellet. The biologic function of this cell surface-expressed 
      viral protein remains unknown.
FAU - Slagle, B L
AU  - Slagle BL
FAU - Butel, J S
AU  - Butel JS
LA  - eng
GR  - CA 09197/CA/NCI NIH HHS/United States
GR  - CA 25215/CA/NCI NIH HHS/United States
GR  - CA 33369/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Immune Sera)
RN  - 0 (Membrane Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 3.2.1.52 (Acetylglucosaminidase)
RN  - EC 3.2.1.96 (Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase)
SB  - IM
MH  - Acetylglucosaminidase
MH  - Animals
MH  - Cell Membrane/analysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Immune Sera
MH  - Immunoenzyme Techniques
MH  - Mammary Neoplasms, Experimental/*microbiology
MH  - Mammary Tumor Virus, Mouse/*metabolism
MH  - Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
MH  - Membrane Proteins/*analysis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Weight
MH  - Viral Proteins/*analysis
PMC - PMC7130887
EDAT- 1985/05/01 00:00
MHDA- 1985/05/01 00:01
PMCR- 2004/02/06
CRDT- 1985/05/01 00:00
PHST- 1985/05/01 00:00 [pubmed]
PHST- 1985/05/01 00:01 [medline]
PHST- 1985/05/01 00:00 [entrez]
PHST- 2004/02/06 00:00 [pmc-release]
AID - 0042-6822(85)90102-3 [pii]
AID - 10.1016/0042-6822(85)90102-3 [doi]
PST - ppublish
SO  - Virology. 1985 May;143(1):127-42. doi: 10.1016/0042-6822(85)90102-3.