PMID- 29980209
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181114
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 14
IP  - 1
DP  - 2018 Jul 6
TI  - Effect of prolonged submaximal exercise on serum oxidative stress biomarkers 
      (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses.
PG  - 216
LID - 10.1186/s12917-018-1540-y [doi]
LID - 216
AB  - BACKGROUND: Oxidative stress (OS) associated with an intense exercise may have a 
      negative influence on equine health. The aim of this study was to determine the 
      effects of endurance races on oxidative and antioxidative status of horses by 
      evaluating changes in reactive oxygen metabolites (d-ROMs), malondialdehyde 
      (MDA), biological antioxidant potential (BAP) and oxidative stress index (OSI) 
      values. The study was carried out on 53 race starts (28 individual horses) 
      competing at different endurance races according to distance (40 and 80 km) and 
      difficulty (easy and demanding). Blood samples were taken before and after the 
      race. RESULTS: Compared to levels of OS serum biomarkers before the race, an 
      increase in values of d-ROMs (P <  0.01), MDA (P <  0.01), and BAP (P <  0.001), 
      and a decrease in OSI (P <  0.001) have been noted after the race. Contrary to 
      other measured biomarkers, BAP did not show significant individual effects of 
      horses. Horses competing at shorter races have shown a significant change in 
      d-ROMs (P = 0.002), BAP (P <  0.001) and OSI (P = 0.004), whereas those competing 
      at longer races in MDA (P = 0.002), BAP (P <  0.001) and OSI (P <  0.001) 
      post-race values. Endurance racing induced changes in values of d-ROMs, BAP and 
      OSI during both easy and demanding races. CONCLUSIONS: Changes in all measured OS 
      biomarkers indicate that prolonged aerobic exercise during endurance race could 
      contribute to the imbalance between oxidants and antioxidants in horses, mainly 
      characterised by a pronounced antioxidant response. Biological antioxidant 
      potential was found to be the most reliable biomarker of OS in endurance horses 
      in the present study.
FAU - Brkljaca Bottegaro, Nika
AU  - Brkljaca Bottegaro N
AUID- ORCID: 0000-0003-1622-0270
AD  - Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary 
      Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia. 
      nikabottegaro@gmail.com.
FAU - Gotic, Jelena
AU  - Gotic J
AD  - Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of 
      Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
FAU - Suran, Jelena
AU  - Suran J
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, 
      University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
FAU - Brozic, Diana
AU  - Brozic D
AD  - Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, 
      University of Zagreb, Heinzelova 55, 10 000, Zagreb, Croatia.
FAU - Klobucar, Karla
AU  - Klobucar K
AD  - Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, 
      Zagreb, Croatia.
FAU - Bojanic, Krunoslav
AU  - Bojanic K
AD  - School of Veterinary Science, Massey University, Palmerston North, New Zealand.
FAU - Vrbanac, Zoran
AU  - Vrbanac Z
AD  - Department of Radiology, Ultrasound Diagnostic and Physical Therapy, Faculty of 
      Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000, Zagreb, 
      Croatia.
LA  - eng
PT  - Journal Article
DEP - 20180706
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Reactive Oxygen Species)
RN  - 4Y8F71G49Q (Malondialdehyde)
SB  - IM
MH  - Animals
MH  - Antioxidants/analysis
MH  - Biomarkers/blood
MH  - Female
MH  - Horses/blood/metabolism/*physiology
MH  - Male
MH  - Malondialdehyde/*blood
MH  - Oxidative Stress/*physiology
MH  - Physical Conditioning, Animal/*physiology
MH  - Reactive Oxygen Species/*blood
PMC - PMC6035461
OTO - NOTNLM
OT  - Biological antioxidant potential
OT  - Endurance race
OT  - Horse
OT  - Malondialdehyde
OT  - Oxidative stress
OT  - Oxidative stress index
OT  - Reactive oxygen metabolites
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: A written consent statement was 
      obtained from the owners of animals included in the study. The Ethical Board of 
      the Faculty of Veterinary Medicine approved the study (File No. 640-01/16-17/75; 
      Record No. 251-61-01/139-16-2). CONSENT FOR PUBLICATION: Not applicable. 
      COMPETING INTERESTS: The authors declare that they have no competing interests. 
      PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional 
      claims in published maps and institutional affiliations.
EDAT- 2018/07/08 06:00
MHDA- 2018/10/16 06:00
PMCR- 2018/07/06
CRDT- 2018/07/08 06:00
PHST- 2018/02/08 00:00 [received]
PHST- 2018/06/24 00:00 [accepted]
PHST- 2018/07/08 06:00 [entrez]
PHST- 2018/07/08 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/07/06 00:00 [pmc-release]
AID - 10.1186/s12917-018-1540-y [pii]
AID - 1540 [pii]
AID - 10.1186/s12917-018-1540-y [doi]
PST - epublish
SO  - BMC Vet Res. 2018 Jul 6;14(1):216. doi: 10.1186/s12917-018-1540-y.