PMID- 29980311
OWN - NLM
STAT- MEDLINE
DCOM- 20200601
LR  - 20200601
IS  - 1532-1983 (Electronic)
IS  - 0261-5614 (Linking)
VI  - 38
IP  - 3
DP  - 2019 Jun
TI  - Genetic variants of tumor necrosis factor-alpha and its levels: A correlation with 
      dyslipidemia and type 2 diabetes susceptibility.
PG  - 1414-1422
LID - S0261-5614(18)31181-6 [pii]
LID - 10.1016/j.clnu.2018.06.962 [doi]
AB  - BACKGROUND & AIM: Tumor necrosis factor-alpha (TNF-alpha) and its genetic variants are 
      implicated in the development of type 2 diabetes (T2D) as a result of systemic 
      inflammation, dyslipidemia, and insulin resistance. The aim of the present study 
      was to investigate i) single nucleotide polymorphisms (SNPs) of TNF-alpha and its 
      association with altered TNF-alpha transcript levels and plasma concentrations ii) 
      free fatty acid (FFA) concentrations as a marker for dyslipidemia and its 
      association with TNF-alpha and iii) genotype-phenotype correlation analysis in T2D 
      patients. METHODS: Plasma and PBMCs were separated from venous blood of 478 
      diabetic patients and 502 age-matched non-diabetic individuals. Genomic DNA was 
      isolated from PBMCs and RNA was isolated from PBMCs and adipose tissue samples. 
      PCR-RFLP was used for genotyping and qPCR to estimate TNF-alpha levels. TNF-alpha and FFA 
      concentrations were estimated from plasma samples by ELISA. RESULTS: Our study 
      suggests: i) involvement of TNF-alpha -857 C/T in T2D patients (p < 0.0001), ii) 
      2.072 and 6.7 fold elevation in TNF-alpha transcript levels in patients' PBMCs and 
      adipose tissues respectively, increased plasma TNF-alpha (p = 0.0122) particularly in 
      obese patients (p = 0.0405), increased plasma FFA (p = 0.0215) and, iii) 
      association of TNF-alpha -238 G/A with body mass index (BMI) (p = 0.0270) 
      and, -857 C/T with fasting blood glucose (FBG) (p = 0.0122) and triglycerides 
      (TG) (p = 0.0015). Correlation analysis suggests that TNF-alpha concentrations are 
      positively correlated with BMI (r = 0.3, p = 0.04) and negatively correlated with 
      HDL (r = -0.39, p = 0.001) while the FFA concentrations are positively correlated 
      with BMI (r = 0.35, p = 0.0004). CONCLUSION: It can be concluded that the genetic 
      variant of TNF-alpha along with elevated TNF-alpha and FFA concentrations play a role in 
      the development of dyslipidemia which could be a potent risk factor towards T2D 
      in Gujarat population.
CI  - Copyright (c) 2018 Elsevier Ltd and European Society for Clinical Nutrition and 
      Metabolism. All rights reserved.
FAU - Patel, Roma
AU  - Patel R
AD  - Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University 
      of Baroda, Vadodara, 390002, Gujarat, India.
FAU - Palit, Sayantani Pramanik
AU  - Palit SP
AD  - Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University 
      of Baroda, Vadodara, 390002, Gujarat, India.
FAU - Rathwa, Nirali
AU  - Rathwa N
AD  - Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University 
      of Baroda, Vadodara, 390002, Gujarat, India.
FAU - Ramachandran, A V
AU  - Ramachandran AV
AD  - Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of 
      Baroda, Vadodara, 390002, Gujarat, India.
FAU - Begum, Rasheedunnisa
AU  - Begum R
AD  - Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University 
      of Baroda, Vadodara, 390002, Gujarat, India. Electronic address: 
      rasheedunnisab@yahoo.co.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180623
PL  - England
TA  - Clin Nutr
JT  - Clinical nutrition (Edinburgh, Scotland)
JID - 8309603
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Diabetes Mellitus, Type 2/*blood/genetics
MH  - Dyslipidemias/*blood/genetics
MH  - Female
MH  - Genetic Association Studies/*methods
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Tumor Necrosis Factor-alpha/*blood/*genetics
OTO - NOTNLM
OT  - Free fatty acid
OT  - Genotype-phenotype correlation
OT  - Single nucleotide polymorphism
OT  - Tumor necrosis factor-alpha
OT  - Type 2 diabetes
OT  - Visceral adipose tissue
EDAT- 2018/07/08 06:00
MHDA- 2020/06/02 06:00
CRDT- 2018/07/08 06:00
PHST- 2018/01/08 00:00 [received]
PHST- 2018/06/04 00:00 [revised]
PHST- 2018/06/13 00:00 [accepted]
PHST- 2018/07/08 06:00 [pubmed]
PHST- 2020/06/02 06:00 [medline]
PHST- 2018/07/08 06:00 [entrez]
AID - S0261-5614(18)31181-6 [pii]
AID - 10.1016/j.clnu.2018.06.962 [doi]
PST - ppublish
SO  - Clin Nutr. 2019 Jun;38(3):1414-1422. doi: 10.1016/j.clnu.2018.06.962. Epub 2018 
      Jun 23.