PMID- 29981934
OWN - NLM
STAT- MEDLINE
DCOM- 20190411
LR  - 20190411
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 21
IP  - 1
DP  - 2019 Jan-Feb
TI  - Mycobacterium tuberculosis acyl carrier protein inhibits macrophage apoptotic 
      death by modulating the reactive oxygen species/c-Jun N-terminal kinase pathway.
PG  - 40-49
LID - S1286-4579(18)30144-8 [pii]
LID - 10.1016/j.micinf.2018.06.005 [doi]
AB  - Mycobacterial acyl carrier protein (AcpM; Rv2244) is a meromycolate extension 
      acyl carrier protein of Mycobacterium tuberculosis (Mtb), which participates in 
      multistep mycolic acid biosynthesis. However, the function of AcpM in 
      host-mycobacterium interactions during infection remains largely uncharacterized. 
      Here we show that AcpM inhibits host cell apoptosis during mycobacterial 
      infection. To examine the function of AcpM during infection, we generated a 
      recombinant Mycobacterium smegmatis (M. smegmatis) strain overexpressing AcpM 
      (Ms_AcpM) and a strain transformed with an empty vector (Ms_Vec). Ms_AcpM 
      promoted intracellular survival of M. smegmatis and led to a significant decrease 
      in the death rate of primary bone marrow-derived macrophages (BMDMs). 
      Importantly, Ms_AcpM showed significantly decreased reactive oxygen species (ROS) 
      generation and activation of c-Jun N-terminal kinase (JNK) signaling compared 
      with Ms_Vec. In addition, treatment of BMDMs with recombinant AcpM significantly 
      inhibited the apoptosis and ROS/JNK signaling induced by M. smegmatis. Moreover, 
      recombinant AcpM enhanced intracellular survival of Mtb H37Rv. Taken together, 
      these results indicate that AcpM plays a role as a virulence factor by modulating 
      host cell apoptosis during mycobacterial infection.
CI  - Copyright (c) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Paik, Seungwha
AU  - Paik S
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea; Department of Medical Science, Chungnam National 
      University School of Medicine, Daejeon 35015, South Korea; Infection Control 
      Convergence Research Center, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea.
FAU - Choi, Seunga
AU  - Choi S
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea; Department of Medical Science, Chungnam National 
      University School of Medicine, Daejeon 35015, South Korea.
FAU - Lee, Kang-In
AU  - Lee KI
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea; Department of Medical Science, Chungnam National 
      University School of Medicine, Daejeon 35015, South Korea.
FAU - Back, Yong Woo
AU  - Back YW
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea; Department of Medical Science, Chungnam National 
      University School of Medicine, Daejeon 35015, South Korea; Infection Control 
      Convergence Research Center, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea.
FAU - Son, Yeo-Jin
AU  - Son YJ
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea.
FAU - Jo, Eun-Kyeong
AU  - Jo EK
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea; Department of Medical Science, Chungnam National 
      University School of Medicine, Daejeon 35015, South Korea; Infection Control 
      Convergence Research Center, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea. Electronic address: hayoungj@cnu.ac.kr.
FAU - Kim, Hwa-Jung
AU  - Kim HJ
AD  - Department of Microbiology, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea; Department of Medical Science, Chungnam National 
      University School of Medicine, Daejeon 35015, South Korea; Infection Control 
      Convergence Research Center, Chungnam National University School of Medicine, 
      Daejeon 35015, South Korea. Electronic address: hjukim@cnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180705
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (AcpM protein, Mycobacterium tuberculosis)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*genetics
MH  - Bacterial Proteins/genetics/*metabolism/pharmacology
MH  - Carrier Proteins/genetics/*metabolism/pharmacology
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression
MH  - JNK Mitogen-Activated Protein Kinases/genetics/*metabolism
MH  - Macrophages/metabolism/microbiology/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Microbial Viability/drug effects
MH  - Mycobacterium Infections/immunology/metabolism/microbiology
MH  - Mycobacterium smegmatis/genetics/physiology
MH  - Mycobacterium tuberculosis/*chemistry/genetics/physiology
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Acyl carrier protein
OT  - Apoptosis
OT  - Mycobacterium smegmatis
OT  - Mycobacterium tuberculosis
OT  - Reactive oxygen species
OT  - c-Jun N-terminal kinase
EDAT- 2018/07/10 06:00
MHDA- 2019/04/12 06:00
CRDT- 2018/07/09 06:00
PHST- 2017/12/04 00:00 [received]
PHST- 2018/03/25 00:00 [revised]
PHST- 2018/06/25 00:00 [accepted]
PHST- 2018/07/10 06:00 [pubmed]
PHST- 2019/04/12 06:00 [medline]
PHST- 2018/07/09 06:00 [entrez]
AID - S1286-4579(18)30144-8 [pii]
AID - 10.1016/j.micinf.2018.06.005 [doi]
PST - ppublish
SO  - Microbes Infect. 2019 Jan-Feb;21(1):40-49. doi: 10.1016/j.micinf.2018.06.005. 
      Epub 2018 Jul 5.