PMID- 29982264
OWN - NLM
STAT- MEDLINE
DCOM- 20181024
LR  - 20181114
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 24
DP  - 2018 Jul 8
TI  - Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis.
PG  - 4704-4710
LID - 10.12659/MSM.908302 [doi]
AB  - BACKGROUND We aimed to investigate the role of T-Helper (TH) 9 cells in the 
      pathogenesis of allergic rhinitis (AR) in mice. MATERIAL AND METHODS An AR model 
      was produced in BALB/c mice, and the viral encoding interleukin (IL)-9 silencing 
      sequence was used to reduce IL-9 expression. The experiment was divided into a 
      control group, an AR group, an IL-9 shRNA+AR group, and a vector+AR group. 
      Hematoxylin and eosin (H&E) staining was used to detect pathological changes. The 
      cytokine expression was detected by ELISA method. Cellular typing was detected by 
      flow cytometry. RESULTS Cells in the control group were regularly arranged, with 
      clear layers and no congestion, edema, or necrosis observable. By contrast, in 
      the AR model group and the vector treatment group, nasal mucosa showed clear 
      hyperemia and edema in upper tissues and infiltration of inflammatory cells, 
      which were ameliorated by IL-9 silencing. Compared with the control group, 
      interferon-gamma (IFN-gamma) was significantly down-regulated, while IL-4, IL-17, and 
      IL-9 were significantly elevated in the AR model group. TH1 cells in nasal 
      mucosa, lymph, nasal lavage, spleen, and peripheral blood were significantly 
      reduced, while TH2, TH9, TH17, and Treg cells were significantly elevated in the 
      AR group compared with the control group. Importantly, all these changes in AR 
      model were ameliorated by IL-9 silencing. CONCLUSIONS AR is related to the 
      changes of cytokines in TH1, TH2, TH9, TH17, and Treg, which are improved by IL-9 
      silencing. Activation of TH9 cells is involved in the pathogenesis of AR.
FAU - Jiang, Xunshuo
AU  - Jiang X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
FAU - Zhang, Xiaona
AU  - Zhang X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Third Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
FAU - Liu, Jianguo
AU  - Liu J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
FAU - Liu, Jiali
AU  - Liu J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
FAU - Zhu, Xinhua
AU  - Zhu X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
FAU - Yang, Chunping
AU  - Yang C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Third Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20180708
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-9)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cytokines/immunology
MH  - Disease Models, Animal
MH  - Interferon-gamma
MH  - Interleukin-17/immunology/metabolism
MH  - Interleukin-4/immunology/metabolism
MH  - Interleukin-9/antagonists & inhibitors/*immunology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nasal Mucosa/immunology
MH  - Rhinitis, Allergic/*immunology/pathology
MH  - T-Lymphocytes, Helper-Inducer/*immunology/metabolism
MH  - T-Lymphocytes, Regulatory/immunology/metabolism
MH  - Th1 Cells/immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
PMC - PMC6069443
EDAT- 2018/07/10 06:00
MHDA- 2018/10/26 06:00
PMCR- 2018/07/08
CRDT- 2018/07/09 06:00
PHST- 2018/07/09 06:00 [entrez]
PHST- 2018/07/10 06:00 [pubmed]
PHST- 2018/10/26 06:00 [medline]
PHST- 2018/07/08 00:00 [pmc-release]
AID - 908302 [pii]
AID - 10.12659/MSM.908302 [doi]
PST - epublish
SO  - Med Sci Monit. 2018 Jul 8;24:4704-4710. doi: 10.12659/MSM.908302.