PMID- 29985005 OWN - NLM STAT- MEDLINE DCOM- 20180814 LR - 20231213 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 66 IP - 30 DP - 2018 Aug 1 TI - Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain. PG - 7935-7941 LID - 10.1021/acs.jafc.8b01581 [doi] AB - Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays an important role in cognitive abilities, including memory and learning. We demonstrated that soybean protein hydrolysate (SPH) diet suppresses age-related cognitive decline via the upregulation of BDNF in a mouse model of senescence. Our purpose was to identify novel bioactive peptides in SPH, which enhance BDNF expression. We treated mouse primary astrocytes with SPH as well as with its positively charged chromatographic fraction. Significant increases in the expression of BDNF were observed in the treatment with positively charged fraction of SPH. Among the synthesized peptides, the dipeptide glycine-arginine (GR) increased BDNF expression in vitro, and LC-TOF-MS analysis showed the presence of GR in the SPH. Furthermore, its administration in vivo increased the expression of BDNF in the cerebral cortex and the number of neurons in hippocampus and cerebral cortex. These data indicate that GR might promote neurogenesis by upregulating BDNF levels. FAU - Shimizu, Ayano AU - Shimizu A AD - Department of Agriculture, Graduate School of Science and Technology , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. FAU - Mitani, Takakazu AU - Mitani T AD - Interdisciplinary Cluster for Cutting Edge Research (ICCER) , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. FAU - Tanaka, Sachi AU - Tanaka S AD - Department of Agriculture, Graduate School of Science and Technology , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. FAU - Fujii, Hiroshi AU - Fujii H AD - Interdisciplinary Cluster for Cutting Edge Research (ICCER) , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. FAU - Maebuchi, Motohiro AU - Maebuchi M AD - Research Institute for Creating the Future , Fuji Oil Holdings Inc. , 4-3, Kinunodai , Tsukuba , Ibaraki 300-2497 , Japan. FAU - Amiya, Yusuke AU - Amiya Y AD - Division of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture , Graduate School, Kyushu University , 744, Motooka , Nishi-ku Fukuoka 819-0395 , Japan. FAU - Tanaka, Mitsuru AU - Tanaka M AD - Division of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture , Graduate School, Kyushu University , 744, Motooka , Nishi-ku Fukuoka 819-0395 , Japan. FAU - Matsui, Toshiro AU - Matsui T AUID- ORCID: 0000-0002-9137-8417 AD - Division of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture , Graduate School, Kyushu University , 744, Motooka , Nishi-ku Fukuoka 819-0395 , Japan. FAU - Nakamura, Soichiro AU - Nakamura S AD - Department of Agriculture, Graduate School of Science and Technology , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. FAU - Katayama, Shigeru AU - Katayama S AUID- ORCID: 0000-0002-7692-0167 AD - Department of Agriculture, Graduate School of Science and Technology , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. AD - Interdisciplinary Cluster for Cutting Edge Research (ICCER) , Shinshu University , 8304 Minamiminowa Kamiina , Nagano 399-4598 , Japan. LA - eng PT - Journal Article DEP - 20180720 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Dipeptides) RN - 0 (Plant Extracts) RN - 94ZLA3W45F (Arginine) RN - TE7660XO1C (Glycine) SB - IM MH - Aging/drug effects/metabolism MH - Animals MH - Arginine/chemistry MH - Brain/*drug effects/metabolism MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - Dipeptides/*administration & dosage/chemistry MH - Female MH - Glycine/chemistry MH - Hippocampus/drug effects/metabolism MH - Humans MH - Male MH - Memory MH - Mice MH - Mice, Inbred C57BL MH - Neurogenesis/drug effects MH - Plant Extracts/*administration & dosage/chemistry MH - Glycine max/*chemistry/metabolism OTO - NOTNLM OT - BDNF OT - astrocytes OT - glycine-arginine dipeptide OT - neurogenesis OT - soybean protein hydrolysate EDAT- 2018/07/10 06:00 MHDA- 2018/08/15 06:00 CRDT- 2018/07/10 06:00 PHST- 2018/07/10 06:00 [pubmed] PHST- 2018/08/15 06:00 [medline] PHST- 2018/07/10 06:00 [entrez] AID - 10.1021/acs.jafc.8b01581 [doi] PST - ppublish SO - J Agric Food Chem. 2018 Aug 1;66(30):7935-7941. doi: 10.1021/acs.jafc.8b01581. Epub 2018 Jul 20.