PMID- 29987690
OWN - NLM
STAT- MEDLINE
DCOM- 20200109
LR  - 20221223
IS  - 1556-0961 (Electronic)
IS  - 1541-6933 (Linking)
VI  - 30
IP  - 1
DP  - 2019 Feb
TI  - Hyperbaric Oxygen Protects Against Cerebral Damage in Permanent Middle Cerebral 
      Artery Occlusion Rats and Inhibits Autophagy Activity.
PG  - 98-105
LID - 10.1007/s12028-018-0577-x [doi]
AB  - BACKGROUND: To investigate the effects of hyperbaric oxygen (HBO) on brain damage 
      and autophagy levels in a rat model of middle cerebral artery occlusion. METHODS: 
      Neurologic injury and infarcted areas were evaluated according to the modified 
      neurological severity score and 2,3,5-triphenyltetrazolium chloride staining. 
      Western blots were used to determine beclin1, caspase-3 and fodrin1 protein 
      expression. Beclin1 protein expression (an autophagy marker), positive terminal 
      dUTP nick-end labeling (TUNEL) staining (an apoptosis marker) and positive 
      propidium iodide (PI) staining (a necrosis marker) were detected by 
      immunofluorescence. RESULTS: Our results indicated that HBO could decrease the 
      infarct volume and speed up the recovery of the neurological deficit scores in 
      ischemic rats. Beclin1 was down-regulated after HBO treatment. HBO treatment 
      inhibited fodrin1 protein expression and decreased the number of PI-positive 
      cells. HBO also down-regulated caspase-3 and decreased the number of 
      TUNEL-positive cells. CONCLUSION: Cerebral ischemia caused early neuronal death 
      due to necrosis, followed by delayed neuronal death due to apoptosis. 
      Consequently, autophagy might be involved in all processes of ischemia. HBO could 
      protect the brain against ischemic injury, and the possible mechanisms might be 
      correlated with decreased autophagy activity and decreased apoptosis and necrosis 
      levels.
FAU - Lu, KongMiao
AU  - Lu K
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
AD  - General ICU, Second Affiliated Hospital, Zhejiang University School of Medicine, 
      Hangzhou, 310052, China.
FAU - Wang, HaiRong
AU  - Wang H
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
FAU - Ge, XiaoLi
AU  - Ge X
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
FAU - Liu, QingHua
AU  - Liu Q
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
FAU - Chen, Miao
AU  - Chen M
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
FAU - Shen, Yong
AU  - Shen Y
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
FAU - Liu, Xuan
AU  - Liu X
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China.
FAU - Pan, ShuMing
AU  - Pan S
AUID- ORCID: 0000-0002-2658-7589
AD  - Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, 200092, China. panshuming@xinhuamed.com.cn.
LA  - eng
GR  - GWDTR201219/Public Health Talent Training Plan of Shanghai/International
GR  - BXJ201726/post the outstanding young medical talent plan of Xinhua hospital. the 
      Doctorial Innovation Fund of Shanghai Jiao Tong University School of 
      Medicine/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurocrit Care
JT  - Neurocritical care
JID - 101156086
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - *Autophagy/physiology
MH  - Brain Ischemia/pathology/physiopathology/*therapy
MH  - Cell Death/physiology
MH  - Disease Models, Animal
MH  - *Hyperbaric Oxygenation
MH  - Infarction, Middle Cerebral Artery/pathology/physiopathology/*therapy
MH  - Male
MH  - Necrosis/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Cerebral infarction
OT  - Hyperbaric oxygen
OT  - Necrosis
EDAT- 2018/07/11 06:00
MHDA- 2020/01/10 06:00
CRDT- 2018/07/11 06:00
PHST- 2018/07/11 06:00 [pubmed]
PHST- 2020/01/10 06:00 [medline]
PHST- 2018/07/11 06:00 [entrez]
AID - 10.1007/s12028-018-0577-x [pii]
AID - 10.1007/s12028-018-0577-x [doi]
PST - ppublish
SO  - Neurocrit Care. 2019 Feb;30(1):98-105. doi: 10.1007/s12028-018-0577-x.