PMID- 29987698
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 70
IP  - 5
DP  - 2018 Oct
TI  - Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and 
      mouse using end sequences of CHO BAC clones based on BAC-FISH results.
PG  - 1399-1407
LID - 10.1007/s10616-018-0233-5 [doi]
AB  - Chinese hamster ovary (CHO) cells have frequently been used in biotechnology as a 
      mammalian host cell platform for expressing genes of interest. Previously, we 
      constructed a detailed physical chromosomal map of the CHO DG44 cell line by 
      fluorescence in situ hybridization (FISH) imaging using 303 bacterial artificial 
      chromosome (BAC) clones as hybridization probes (BAC-FISH). BAC-FISH results 
      revealed that the two longest chromosomes were completely paired. However, other 
      chromosomes featured partial deletions or rearrangements. In this study, we 
      determined the end sequences of 303 BAC clones (BAC end sequences), which were 
      used for BAC-FISH probes. Among 606 BAC-end sequences (BESs) (forward and reverse 
      ends), 558 could be determined. We performed a comparison between all determined 
      BESs and mouse genome sequences using NCBI BLAST. Among these 558 BESs, 465 
      showed high homology to mouse chromosomal sequences. We analyzed the locations of 
      these BACs in chromosomes of the CHO DG44 cell line using a physical chromosomal 
      map. From the obtained results, we investigated the regional similarities among 
      CHO chromosomes (A-T) and mouse chromosomes (1-19 and sex) about 217 BESs (46.7% 
      of 465 high homologous BESs). Twenty-three specific narrow regions in 13 
      chromosomes of the CHO DG44 cell line showed high homology to mouse chromosomes, 
      but most of other regions did not show significant correlations with the mouse 
      genome. These results contribute to accurate alignments of chromosomes of Chinese 
      hamster and its genome sequence, analysis of chromosomal instability in CHO 
      cells, and the development of target locations for gene and/or genome editing 
      techniques.
FAU - Kimura, Shuichi
AU  - Kimura S
AD  - Graduate School of Advanced Technology and Science, Tokushima University, 
      Tokushima, 770-8506, Japan.
FAU - Omasa, Takeshi
AU  - Omasa T
AUID- ORCID: 0000-0003-0635-3862
AD  - Graduate School of Advanced Technology and Science, Tokushima University, 
      Tokushima, 770-8506, Japan. omasa@bio.eng.osaka-u.ac.jp.
AD  - Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, 770-8506, 
      Japan. omasa@bio.eng.osaka-u.ac.jp.
AD  - Graduate School of Engineering, Osaka University, Osaka, 565-0871, Japan. 
      omasa@bio.eng.osaka-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20180709
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC6214854
OTO - NOTNLM
OT  - Aneuploidy
OT  - BAC end sequence
OT  - Chinese hamster ovary (CHO) cell
OT  - Chromosome
OT  - Fluorescence in situ hybridization
OT  - Genome sequence comparison
EDAT- 2018/07/11 06:00
MHDA- 2018/07/11 06:01
PMCR- 2019/10/01
CRDT- 2018/07/11 06:00
PHST- 2018/03/20 00:00 [received]
PHST- 2018/06/16 00:00 [accepted]
PHST- 2018/07/11 06:00 [pubmed]
PHST- 2018/07/11 06:01 [medline]
PHST- 2018/07/11 06:00 [entrez]
PHST- 2019/10/01 00:00 [pmc-release]
AID - 10.1007/s10616-018-0233-5 [pii]
AID - 233 [pii]
AID - 10.1007/s10616-018-0233-5 [doi]
PST - ppublish
SO  - Cytotechnology. 2018 Oct;70(5):1399-1407. doi: 10.1007/s10616-018-0233-5. Epub 
      2018 Jul 9.