PMID- 29988579
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Metal-Adapted Bacteria Isolated From Wastewaters Produce Biofilms by Expressing 
      Proteinaceous Curli Fimbriae and Cellulose Nanofibers.
PG  - 1334
LID - 10.3389/fmicb.2018.01334 [doi]
LID - 1334
AB  - Bacterial biofilm plays a pivotal role in bioremediation of heavy metals from 
      wastewaters. In this study, we isolated and identified different biofilm 
      producing bacteria from wastewaters. We also characterized the biofilm matrix 
      [i.e., extracellular polymeric substances (EPS)] produced by different bacteria. 
      Out of 40 isolates from different wastewaters, only 11 (27.5%) isolates (static 
      condition at 28 degrees C) and 9 (22.5%) isolates (agitate and static conditions at 28 
      and 37 degrees C) produced air-liquid (AL) and solid-air-liquid (SAL) biofilms, 
      respectively, only on salt-optimized broth plus 2% glycerol (SOBG) but not in 
      other media tested. Biomass biofilms and bacteria coupled with AL biofilms were 
      significantly (P </= 0.001) varied in these isolates. Escherichia coli (isolate 
      ENSD101 and ENST501), Enterobacter asburiae (ENSD102), Enterobacter ludwigii 
      (ENSH201), Pseudomonas fluorescens (ENSH202 and ENSG304), uncultured Vitreoscilla 
      sp. (ENSG301 and ENSG305), Acinetobacter lwoffii (ENSG302), Klebsiella pneumoniae 
      (ENSG303), and Bacillus thuringiensis (ENSW401) were identified based on 16S rRNA 
      gene sequencing. Scanning electron microscope (SEM) images revealed that biofilm 
      matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. ENSG301, A. 
      lwoffii ENSG302, and K. pneumoniae ENSG303 are highly fibrous, compact, and 
      nicely interlinked as compared to the biofilm developed by E. ludwigii ENSH201 
      and B. thuringiensis ENSW401. X-ray diffraction (XRD) results indicated that 
      biofilm matrix produced by E. asburiae ENSD102, uncultured Vitreoscilla sp. 
      ENSG301, and A. lwoffii ENSG302 are non-crystalline amorphous nature. Fourier 
      transform infrared (FTIR) spectroscopy showed that proteins and polysaccharides 
      are the main components of the biofilms. Congo red binding results suggested that 
      all these bacteria produced proteinaceous curli fimbriae and cellulose-rich 
      polysaccharide. Production of cellulose was also confirmed by Calcofluor binding- 
      and spectrophotometric assays. E. asburiae ENSD102, Vitreoscilla sp. ENSG301, and 
      A. lwoffii ENSG302 were tested for their abilities to form the biofilms exposure 
      to 0 to 2000 mg/L of copper sulfate (for Cu), zinc sulfate (for Zn), lead nitrate 
      (for Pb), nickel chloride (for Ni), and potassium dichromate (for Cr), several 
      concentrations of these metals activated the biofilm formation. The 
      polysaccharides is known to sequester the heavy metals thus, these bacteria might 
      be applied to remove the heavy metals from wastewater.
FAU - Mosharaf, M K
AU  - Mosharaf MK
AD  - Department of Environmental Science, Faculty of Agriculture, Bangabandhu Sheikh 
      Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Tanvir, M Z H
AU  - Tanvir MZH
AD  - Department of Environmental Science, Faculty of Agriculture, Bangabandhu Sheikh 
      Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Haque, M M
AU  - Haque MM
AD  - Department of Environmental Science, Faculty of Agriculture, Bangabandhu Sheikh 
      Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Haque, M A
AU  - Haque MA
AD  - Department of Agro-Processing, Faculty of Agriculture, Bangabandhu Sheikh Mujibur 
      Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Khan, M A A
AU  - Khan MAA
AD  - Department of Plant Pathology, Faculty of Agriculture, Bangabandhu Sheikh Mujibur 
      Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Molla, A H
AU  - Molla AH
AD  - Department of Environmental Science, Faculty of Agriculture, Bangabandhu Sheikh 
      Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Alam, Mohammad Z
AU  - Alam MZ
AD  - Department of Environmental Science, Faculty of Agriculture, Bangabandhu Sheikh 
      Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
FAU - Islam, M S
AU  - Islam MS
AD  - Bangladesh Jute Research Institute, Dhaka, Bangladesh.
FAU - Talukder, M R
AU  - Talukder MR
AD  - Department of Environmental Science, Faculty of Agriculture, Bangabandhu Sheikh 
      Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
LA  - eng
PT  - Journal Article
DEP - 20180625
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6026672
OTO - NOTNLM
OT  - biofilm
OT  - cellulose
OT  - curli fimbriae
OT  - extracellular polymeric substance
OT  - heavy metal
OT  - wastewater
EDAT- 2018/07/11 06:00
MHDA- 2018/07/11 06:01
PMCR- 2018/06/25
CRDT- 2018/07/11 06:00
PHST- 2018/02/18 00:00 [received]
PHST- 2018/05/31 00:00 [accepted]
PHST- 2018/07/11 06:00 [entrez]
PHST- 2018/07/11 06:00 [pubmed]
PHST- 2018/07/11 06:01 [medline]
PHST- 2018/06/25 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.01334 [doi]
PST - epublish
SO  - Front Microbiol. 2018 Jun 25;9:1334. doi: 10.3389/fmicb.2018.01334. eCollection 
      2018.