PMID- 29991449
OWN - NLM
STAT- MEDLINE
DCOM- 20190404
LR  - 20211204
IS  - 1873-2968 (Electronic)
IS  - 0006-2952 (Linking)
VI  - 155
DP  - 2018 Sep
TI  - Protostemonine attenuates alternatively activated macrophage and DRA-induced 
      asthmatic inflammation.
PG  - 198-206
LID - S0006-2952(18)30259-4 [pii]
LID - 10.1016/j.bcp.2018.07.003 [doi]
AB  - Asthma is one of the most common pulmonary diseases that threatens human life 
      because of lack of effective medicines. Protostemonine (PSN), an active alkaloid 
      extracted from the roots of Stemona sesslifolia, has anti-inflammatory effects on 
      acute lung injury and acute liver failure. However, it has not been defined 
      whether PSN alleviates asthmatic inflammation. Here, we reported that PSN 
      inhibits pulmonary eosinophil infiltration, goblet cell hyperplasia, mucus 
      secretion, IgE and Th2 cytokine (IL-4, IL-5, IL-13 and IL-33) production by using 
      DRA (dust mites, ragweed and aspergillus)-induced murine asthma model. Moreover, 
      PSN also attenuated the expression of Arginase-1 (Arg-1), Ym-1 and Fizz-1, 
      markers of AAM (alternatively activated macrophage) polarization, in lung 
      tissues. In addition, PSN attenuated IL-4-induced expression of Arg-1, Ym-1 and 
      Fizz-1 in bone marrow derived macrophages (BMDMs). Treatment with PSN decreased 
      IL-4-induced STAT6 phosphorylation, KLF4 and IRF4 expression in BMDMs. 
      Collectively, our results indicated that PSN ameliorates AAM polarization and 
      asthmatic inflammation and might be a potential agent for treating asthma.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Song, Yunduan
AU  - Song Y
AD  - Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, 
      Shanghai 201399, PR China.
FAU - Wu, Yaxian
AU  - Wu Y
AD  - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of 
      Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR 
      China. Electronic address: yaxianwu@sjtu.edu.cn.
FAU - Li, Xiaozong
AU  - Li X
AD  - Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, 
      Shanghai 201399, PR China.
FAU - Shen, Yao
AU  - Shen Y
AD  - Department of Respiratory Medicine, Shanghai Pudong Hospital, Fudan University, 
      Shanghai 201399, PR China.
FAU - Ding, Yunhe
AU  - Ding Y
AD  - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of 
      Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR 
      China. Electronic address: yhding1993@sjtu.edu.cn.
FAU - Zhu, Hongbo
AU  - Zhu H
AD  - Department of Pathology, Shanghai Pudong Hospital, Fudan University ,Shanghai 
      201399, PR China.
FAU - Liu, Fangfang
AU  - Liu F
AD  - Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, 
      Shanghai 201399, PR China.
FAU - Yu, Kaikai
AU  - Yu K
AD  - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of 
      Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR 
      China.
FAU - Sun, Lei
AU  - Sun L
AD  - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of 
      Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR 
      China. Electronic address: sunlei_vicky@sjtu.edu.cn.
FAU - Qian, Feng
AU  - Qian F
AD  - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of 
      Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR 
      China; Anhui Province Key Laboratory of Translational Cancer Research, Bengbu 
      Medical College, Bengbu, Anhui Province 233003, PR China. Electronic address: 
      fengqian@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180702
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (KLF4 protein, human)
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Ambrosia/*adverse effects
MH  - Animals
MH  - *Aspergillus fumigatus
MH  - Asthma/chemically induced/*drug therapy/metabolism
MH  - Cell Survival/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Inflammation/chemically induced/drug therapy/metabolism
MH  - Kruppel-Like Factor 4
MH  - Macrophages/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Plant Extracts/isolation & purification/pharmacology/*therapeutic use
MH  - *Pyroglyphidae
MH  - Random Allocation
MH  - Stemonaceae
OTO - NOTNLM
OT  - Alternatively activated macrophage
OT  - Asthmatic inflammation
OT  - IL-4
OT  - PSN
OT  - STAT6
EDAT- 2018/07/12 06:00
MHDA- 2019/04/05 06:00
CRDT- 2018/07/12 06:00
PHST- 2018/05/08 00:00 [received]
PHST- 2018/07/01 00:00 [accepted]
PHST- 2018/07/12 06:00 [pubmed]
PHST- 2019/04/05 06:00 [medline]
PHST- 2018/07/12 06:00 [entrez]
AID - S0006-2952(18)30259-4 [pii]
AID - 10.1016/j.bcp.2018.07.003 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 2018 Sep;155:198-206. doi: 10.1016/j.bcp.2018.07.003. Epub 
      2018 Jul 2.