PMID- 2999187
OWN - NLM
STAT- MEDLINE
DCOM- 19860117
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 22
IP  - 6
DP  - 1985 Dec
TI  - Effect of heat and fresh human serum on the infectivity of human T-cell 
      lymphotropic virus type III evaluated with new bioassay systems.
PG  - 908-11
AB  - MT-4 cells, which are a human T-cell lymphotropic virus type I (HTLV-I)-positive 
      cell line highly permissive to HTLV-III infection, were used to detect the 
      biologically active virus. For quantitation of the virus, induction of 
      HTLV-III-specific antigen(s) and inhibition of DNA synthesis in infected MT-4 
      cells were assessed by indirect immunofluorescence and by a proliferation assay 
      measuring [3H]thymidine uptake, respectively. HTLV-III was fully inactivated by 
      treatment at 56 degrees C for 30 min. It was not inactivated by treatment with 
      fresh anti-HTLV-III-negative serum. Thus, these assay systems with MT-4 cells 
      would be useful in further studies on acquired immune deficiency syndrome.
FAU - Harada, S
AU  - Harada S
FAU - Yoshiyama, H
AU  - Yoshiyama H
FAU - Yamamoto, N
AU  - Yamamoto N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Biological Assay/*methods
MH  - Blood/microbiology
MH  - Cell Line
MH  - Complement System Proteins
MH  - Cytopathogenic Effect, Viral
MH  - Deltaretrovirus/*isolation & purification
MH  - Evaluation Studies as Topic
MH  - Hot Temperature
MH  - Humans
MH  - Lymphocyte Activation
MH  - T-Lymphocytes
PMC - PMC271849
EDAT- 1985/12/01 00:00
MHDA- 1985/12/01 00:01
PMCR- 1985/12/01
CRDT- 1985/12/01 00:00
PHST- 1985/12/01 00:00 [pubmed]
PHST- 1985/12/01 00:01 [medline]
PHST- 1985/12/01 00:00 [entrez]
PHST- 1985/12/01 00:00 [pmc-release]
AID - 10.1128/jcm.22.6.908-911.1985 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1985 Dec;22(6):908-11. doi: 10.1128/jcm.22.6.908-911.1985.