PMID- 29992506
OWN - NLM
STAT- MEDLINE
DCOM- 20190102
LR  - 20190102
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 41
IP  - 5
DP  - 2018 Oct
TI  - Progranulin Is Positively Associated with Intervertebral Disc Degeneration by 
      Interaction with IL-10 and IL-17 Through TNF Pathways.
PG  - 1852-1863
LID - 10.1007/s10753-018-0828-1 [doi]
AB  - Progranulin (PGRN) is a widely expressed growth factor that effectively inhibits 
      tumor necrosis factor alpha (TNFalpha)-mediated inflammatory response. TNFalpha is involved 
      in intervertebral disc degeneration (IDD) and plays a key role. This study aims 
      to determine the role of PGRN in the intervertebral disc degeneration process. We 
      collected intervertebral discs (IVDs) from humans and mice with different genetic 
      backgrounds. We examined the expression of PGRN in IVD tissues by 
      immunohistochemistry staining and Western blotting assay. We examined the 
      peripheral serum level of PGRN by ELISA assay. Murine IVD tissue samples were 
      taken to undergo safranin O, HE, and immunohistochemistry staining. Primary human 
      nucleus pulposus cells were used for ELISA and RT-PCR assays. PGRN as well as 
      interlukin-10 (IL-10) and interlukin-17 (IL-17) expressions were elevated in 
      degenerative discs and peripheral blood sera. Loss of PGRN led to accelerated 
      disc degeneration in the animal model, along with decreased expression of IL-10 
      and increased expression of IL-17. Additionally, the PGRN level was positively 
      related to levels of IL-10 and IL-17. In vitro study suggested that PGRN 
      protected against disc degeneration by inducing IL-10 and reducing IL-17. PGRN is 
      associated with intervertebral disc degeneration through interfering with IL-10 
      and IL-17; thus, PGRN could be an interesting biomarker for diagnosis and a 
      potential treatment target.
FAU - Wang, Shaoyi
AU  - Wang S
AD  - Department of Orthopaedics, Qilu Hospital of Shandong University, No.107, Wen Hua 
      Xi Road, Jinan, Shandong, 250012, People's Republic of China.
AD  - School of Medicine, Shandong University, Jinan, China.
FAU - Wei, Jianlu
AU  - Wei J
AD  - Department of Orthopaedics, Qilu Hospital of Shandong University, No.107, Wen Hua 
      Xi Road, Jinan, Shandong, 250012, People's Republic of China.
FAU - Fan, Yuchen
AU  - Fan Y
AD  - School of Medicine, Shandong University, Jinan, China.
FAU - Ding, Hong
AU  - Ding H
AD  - Department of Orthopaedics, Qilu Hospital of Shandong University, No.107, Wen Hua 
      Xi Road, Jinan, Shandong, 250012, People's Republic of China.
AD  - School of Medicine, Shandong University, Jinan, China.
FAU - Tian, Huichao
AU  - Tian H
AD  - Department of Orthopaedics, Qilu Hospital of Shandong University, No.107, Wen Hua 
      Xi Road, Jinan, Shandong, 250012, People's Republic of China.
AD  - School of Medicine, Shandong University, Jinan, China.
FAU - Zhou, Xiaocong
AU  - Zhou X
AD  - School of Medicine, Shandong University, Jinan, China.
FAU - Cheng, Lei
AU  - Cheng L
AD  - Department of Orthopaedics, Qilu Hospital of Shandong University, No.107, Wen Hua 
      Xi Road, Jinan, Shandong, 250012, People's Republic of China. chengleiyx@126.com.
LA  - eng
GR  - 81572191/National Natural Science Foundation of China/
PT  - Journal Article
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Interleukin-17)
RN  - 0 (Progranulins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Interleukin-10/*metabolism
MH  - Interleukin-17/*metabolism
MH  - Intervertebral Disc/metabolism/pathology
MH  - Intervertebral Disc Degeneration/diagnosis/*metabolism
MH  - Mice
MH  - Progranulins/*analysis
MH  - Tumor Necrosis Factor-alpha/*metabolism
OTO - NOTNLM
OT  - IL-10
OT  - IL-17
OT  - TNFalpha
OT  - intervertebral disc degeneration
OT  - progranulin
EDAT- 2018/07/12 06:00
MHDA- 2019/01/03 06:00
CRDT- 2018/07/12 06:00
PHST- 2018/07/12 06:00 [pubmed]
PHST- 2019/01/03 06:00 [medline]
PHST- 2018/07/12 06:00 [entrez]
AID - 10.1007/s10753-018-0828-1 [pii]
AID - 10.1007/s10753-018-0828-1 [doi]
PST - ppublish
SO  - Inflammation. 2018 Oct;41(5):1852-1863. doi: 10.1007/s10753-018-0828-1.